RESUMO
The possibility to recover the antioxidant system in rats with experimental liver cirrhosis (LC) after allo- and xenotransplantation of cryopreserved fetal liver cells (FLC) was investigated. It was shown that the content of lipid peroxidation products in the blood serum of animals with LC four weeks after FLC transplantation decreased significantly as compared to control group. Such changes were accompanied by a significant increase of catalase (CAT), glutathione reductase (GR), Se-dependent glutathione peroxidase (GP) activity in the liver and total anti-oxidative activity (AOA) of blood. Obtained results demonstrate that the main direction of FLC effects in animals with LC agree with that we observed previously in other experimental models (partial hepatectomy, chronic alcohol poisoning and hypercholesterolemia). In conclusion, cell therapy may be considered as the universal method for correction of disorders in regulation of free-radical processes in various experimental pathologies.
Assuntos
Antioxidantes/metabolismo , Transplante de Células , Criopreservação , Cirrose Hepática Experimental/cirurgia , Fígado/citologia , Animais , Feto , Humanos , Peróxidos Lipídicos/sangue , Peróxidos Lipídicos/metabolismo , Fígado/embriologia , Fígado/enzimologia , Fígado/metabolismo , Cirrose Hepática Experimental/sangue , Cirrose Hepática Experimental/enzimologia , Masculino , Ratos , Transplante Heterólogo , Transplante Homólogo , Resultado do TratamentoRESUMO
The model of partial hepatectomy was utilized to investigate the influence of human fetal liver cells (FLCs) and of human embryo tissue (PCF) postnuclear cytoplasm fraction injection on the rate of DNA and nRNA synthesis in regenerating rat liver cells. Single infusion of FLCs and PCF into the spleen pulp of rats has been shown to increase the DNA synthesis 24 hours after the operation in 2.5 +/- 0.4 and 3.2 +/- 0.5 times respectively. A group of rats has been identified with no influence of the infusion on the DNA synthesis 24 hours after partial hepatectomy, this process having been even retarding in 48 hours after the operation. Meanwhile the FLCs and PCF infusion enhanced the intensity of nRNA synthesis in 72 hours after the operation in all the animal groups. The effect demonstrated is probably caused by the biologically active substances contained in the fetal tissues.
Assuntos
DNA/biossíntese , Transplante de Tecido Fetal , Hepatócitos/citologia , Regeneração Hepática/fisiologia , RNA Nuclear/biossíntese , Animais , Núcleo Celular/metabolismo , Hepatectomia , Hepatócitos/fisiologia , Humanos , Cinética , Fígado/embriologia , Transplante de Fígado/métodos , Ratos , Ratos Wistar , Baço/citologia , Baço/metabolismoRESUMO
We studied the effect of transplantation of fetal liver cells and postnuclear cytoplasmic fraction from human fetal soft tissues on the prooxidant-antioxidant equilibrium in the liver and blood of rats after partial hepatectomy. The preparations increased antioxidant activity and decreased the intensity of lipid peroxidation, which probably contributes to their therapeutic effects.