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1.
Insect Biochem Mol Biol ; 167: 104086, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38295885

RESUMO

Growth-blocking peptide (GBP), an insect cytokine, was first found in armyworm Mythimna separata. A functional analogue of GBP, stress-responsive peptide (SRP), was also identified in the same species. SRP gene expression has been demonstrated to be enhanced by GBP, indicating that both cytokines are organized within a hierarchical regulatory network. Although GBP1 (CG15917) and GBP2 (CG11395) have been identified in Drosophila melanogaster, immunological functions have only been characterized for GBP1. It is expected that the biological responses of two structurally similar peptides should be coordinated, but there is little information on this topic. Here, we demonstrate that GBP2 replicates the GBP1-mediated cellular immune response from Drosophila S2 cells. Moreover, the GBP2-induced response was silenced by pre-treatment with dsRNA targeting the GBP receptor gene, Mthl10. Furthermore, treatment of S2 cells with GBP2 enhanced GBP1 expression levels, but GBP1 did not affect GBP2 expression. GBP2 derived enhancement of GBP1 expression was not observed in the presence of GBP1, indicating that GBP2 is an upstream expressional regulator of a GBP1/GBP2 cytokine network. GBP2-induced enhancement of GBP1 expression was not observed in Mthl10 knockdown cells. Enhancement of GBP2 expression was observed in both Drosophila larvae and S2 cells under heat stress conditions; expressional enhancement of both GBP1 and GBP2 was eliminated in Mthl10 knockdown cells and larvae. Finally, Ca2+ mobilization assay in GCaMP3-expressing S2 cells demonstrated that GBP2 mobilizes Ca2+ upstream of Mthl10. Our finding revealed that Drosophila GBP1 and GBP2 control immune responses as well as their own expression levels through a hierarchical cytokine network, indicating that Drosophila GBP1/GBP2 system can be a simple model that is useful to investigate the detailed regulatory mechanism of related cytokine complexes.


Assuntos
Citocinas , Drosophila , Animais , Drosophila/metabolismo , Citocinas/metabolismo , Drosophila melanogaster/metabolismo , Proteínas de Transporte/metabolismo , Peptídeos/metabolismo , Imunidade
2.
Pest Manag Sci ; 80(2): 426-432, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-37714819

RESUMO

BACKGROUND: Booklice Liposcelis bostrychophila are frequently found almost everywhere, including private houses and cleanrooms of factories and institutes. They often cause serious hygienic as well as agricultural problems, but a useful trap has not been developed so far. Therefore, an effective way to monitor and capture booklice is required. RESULTS: We here identified a new attractant, 2,3,5,6-tetramethylpyrazine (TMP), which efficiently captured booklice in combination with UV light. When booklice placed at both right and left edges of an assay tray were exposed to light stimulus from the center, test insects gathered at the center. The attraction was stronger with shorter wavelengths than longer ones: 365-nm ultraviolet (UV) light showed the strongest attraction of four tested light wavelengths. We found that cocoa powder attracted booklice weakly but significantly under total darkness. Furthermore, the cocoa smell was confirmed to enhance the attraction to light at all tested wavelengths irrespective of the difference between two brands of cocoa powders. Gas chromatography-mass spectrometry indicated that both cocoa products contain TMP as a major odor compound. Exposure of booklice to TMP significantly enhanced the attraction to UV light: the combined use with TMP almost doubled the attraction compared to the light only. By contrast, TMP homologs, pyrazine and dimethylpyrazines, showed strong repellent activities under UV light exposure. CONCLUSION: TMP enhanced the UV light attraction for booklice while pyrazine and dimethylpyrazines diminished it. Use of these attractant and repellent pyrazine derivatives together with UV light would enable us to develop a practical new way to monitor and capture booklice. © 2023 Society of Chemical Industry.


Assuntos
Repelentes de Insetos , Raios Ultravioleta , Animais , Insetos , Pirazinas/farmacologia , Repelentes de Insetos/farmacologia
3.
J Biochem ; 174(5): 433-440, 2023 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-37500079

RESUMO

RNA aptamersare nucleic acids that are obtained using the systematic evolution of ligands by exponential enrichment (SELEX) method. When using conventional selection methods to immobilize target proteins on matrix beads using protein tags, sequences are obtained that bind not only to the target proteins but also to the protein tags and matrix beads. In this study, we performed SELEX using ß-1,3-glucan recognition protein (GRP)-tags and curdlan beads to immobilize the acute myeloid leukaemia 1 (AML1) Runt domain (RD) and analysed the enrichment of aptamers using high-throughput sequencing. Comparison of aptamer enrichment using the GRP-tag and His-tag suggested that aptamers were enriched using the GRP-tag as well as using the His-tag. Furthermore, surface plasmon resonance analysis revealed that the aptamer did not bind to the GRP-tag and that the conjugation of the GRP-tag to RD weakened the interaction between the aptamer and RD. The GRP-tag could have acted as a competitor to reduce weakly bound RNAs. Therefore, the affinity system of the GRP-tagged proteins and curdlan beads is suitable for obtaining specific aptamers using SELEX.


Assuntos
Aptâmeros de Nucleotídeos , beta-Glucanas , Glucanos , Aptâmeros de Nucleotídeos/genética , Aptâmeros de Nucleotídeos/metabolismo , RNA , Ligantes
4.
FEBS Lett ; 597(2): 288-297, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36527170

RESUMO

Mitohormesis defines the increase in fitness induced by adaptive responses to mild mitochondrial stress. Here, we show that N-acetyloxfenicine (NAO) exerted higher thermotolerance than an endogenous mitohormesis inducer, N-acetyltyrosine (NAT). This activity was not observed in armyworm larvae injected with oxfenicine, suggesting the importance of N-acetylation. NAO-induced hormetic effect was triggered by transient perturbation of mitochondria, which causes a small increase in ROS production and leads to retrograde responses including enhanced expression of antioxidant enzyme genes via activation of FoxO transcription factors. Furthermore, pretreatment with NAO significantly repressed stress-induced peroxidation of lipids in mice and growth of colorectal cancer HCT116 cells that had been transplanted into nude mice. Taken together, NAO is a potent mitohormesis inducer that is similar to NAT in terms of structure and functions.


Assuntos
Antioxidantes , Mitocôndrias , Animais , Camundongos , Camundongos Nus , Mitocôndrias/metabolismo , Antioxidantes/metabolismo , Transdução de Sinais , Insetos/metabolismo , Espécies Reativas de Oxigênio/metabolismo
5.
J Insect Physiol ; 131: 104225, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33736983

RESUMO

Insect cytokine growth blocking peptide (GBP) is synthesized as an inactive precursor, termed proGBP, that is normally present in a significant concentration in the hemolymph of non-stressed animals (Hayakawa, 1990, 1991). Under stress conditions, proGBP is instantly processed to active GBP by a serine protease and this is thought to be an important initial step for insects to cope with stress-induced adverse effects via GBP-induced physiological changes. However, the detailed mechanism underlying proteolytic processing of hemolymph proGBP in insects under stress conditions remains unknown. Here we demonstrated that proGBP processing requires ROS-induced release of a proteinaceous factor from hemocytes that activates the inactive proGBP processing enzyme. The release of the activator protein from hemocytes is initiated by an elevation of the cytoplasmic Ca2+ concentration induced by ROS. Therefore, we concluded that stress-induced activation of proGBP requires ROS-dependent stimulation of an intracellular calcium signaling pathway in hemocytes, followed by release of the hemocyte proteinaceous factor that specifically activates the proGBP processing enzyme.


Assuntos
Citocinas/metabolismo , Hemócitos/metabolismo , Proteínas de Insetos/metabolismo , Mariposas/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Estresse Fisiológico , Animais , Sinalização do Cálcio
6.
Artigo em Inglês | MEDLINE | ID: mdl-29218733

RESUMO

Recovery from weight loss after stress is important for all organisms, although the recovery mechanisms are not fully understood. We are working to clarify these mechanisms. Here, we recorded enhanced feeding activity of Drosophila melanogaster larvae from 2 to 4 h after heat stress at 35°C for 1 h. During the post-stress period, expression levels of sweet taste gustatory receptor genes (Grs), Gr5a, Gr43a, Gr64a, and Gr64f, were elevated, whereas bitter taste Grs, Gr66a, and Gr33a, were decreased in expression and expression of a non-typical taste receptor Gr, Gr68a, was unchanged. Similar upregulation of Gr5a and downregulation of Gr66a was recorded after cold stress at 4°C. Expression levels of tropomyosin and ATP synthase ß subunit were significantly increased in larval mouth parts around 3 to 5 h after the heat stress. We infer that up-regulation of post-stress larval feeding activity, and weight recovery, is mediated by increasing capacity for mouth part muscular movements and changes in taste sensing physiology. We propose that Drosophila larvae, and likely insects generally, express an efficient mechanism to recover from weight loss during post-stress periods.


Assuntos
Proteínas de Drosophila/metabolismo , Drosophila melanogaster/fisiologia , Ingestão de Alimentos , Receptores de Superfície Celular/metabolismo , Estresse Fisiológico , Animais , Proteínas de Drosophila/genética , Temperatura Alta , Larva/fisiologia , Receptores de Superfície Celular/genética , Redução de Peso
7.
Proc Natl Acad Sci U S A ; 114(52): 13786-13791, 2017 12 26.
Artigo em Inglês | MEDLINE | ID: mdl-29229844

RESUMO

A systems-level understanding of cytokine-mediated, intertissue signaling is one of the keys to developing fundamental insight into the links between aging and inflammation. Here, we employed Drosophila, a routine model for analysis of cytokine signaling pathways in higher animals, to identify a receptor for the growth-blocking peptide (GBP) cytokine. Having previously established that the phospholipase C/Ca2+ signaling pathway mediates innate immune responses to GBP, we conducted a dsRNA library screen for genes that modulate Ca2+ mobilization in Drosophila S3 cells. A hitherto orphan G protein coupled receptor, Methuselah-like receptor-10 (Mthl10), was a significant hit. Secondary screening confirmed specific binding of fluorophore-tagged GBP to both S3 cells and recombinant Mthl10-ectodomain. We discovered that the metabolic, immunological, and stress-protecting roles of GBP all interconnect through Mthl10. This we established by Mthl10 knockdown in three fly model systems: in hemocyte-like Drosophila S2 cells, Mthl10 knockdown decreases GBP-mediated innate immune responses; in larvae, Mthl10 knockdown decreases expression of antimicrobial peptides in response to low temperature; in adult flies, Mthl10 knockdown increases mortality rate following infection with Micrococcus luteus and reduces GBP-mediated secretion of insulin-like peptides. We further report that organismal fitness pays a price for the utilization of Mthl10 to integrate all of these various homeostatic attributes of GBP: We found that elevated GBP expression reduces lifespan. Conversely, Mthl10 knockdown extended lifespan. We describe how our data offer opportunities for further molecular interrogation of yin and yang between homeostasis and longevity.


Assuntos
Citocinas/metabolismo , Proteínas de Drosophila/metabolismo , Longevidade/fisiologia , Receptores Acoplados a Proteínas G/metabolismo , Estresse Fisiológico/fisiologia , Animais , Citocinas/genética , Proteínas de Drosophila/genética , Drosophila melanogaster , Receptores Acoplados a Proteínas G/genética
8.
Zoological Lett ; 3: 3, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28331632

RESUMO

BACKGROUND: Facultative parthenogenesis, seen in many animal phyla, is a reproductive strategy in which females are able to generate offspring when mating partners are unavailable. In some subsocial and eusocial insects, parthenogenesis is often more prevalent than sexual reproduction. However, little is known about how social cooperation is linked to the promotion of parthenogenesis. The domiciliary cockroach Periplaneta americana is well-suited to addressing this issue as this species belongs to the superfamily Blattoidea, which diverged into eusocial termites and shows facultative parthenogenesis. RESULTS: We studied environmental factors that influence asexual production of ootheca using behavioral assays in P. americana. When more than three virgin females immediately after the imaginal molt were kept together in a small sealed container, they tended to produce egg cases (oothecae) via parthenogenesis earlier than did isolated females, resulting in apparent synchronization of ootheca production, even among females housed in different containers. In contrast, virgin females housed with genitalia-ablated males or group-housed females with antennae ablated did not significantly promote ootheca production compared to isolated females. Daily addition of the primary sex pheromone component to the container did not promote ootheca production in isolated females. Another line of study showed that grouped females make parthenogenesis more sustainable than previously known; a founder colony of 15 virgin females was sufficient to produce female progeny for a period of more than three years. CONCLUSIONS: Group-housed females promote and stabilize asexual ootheca production compared to isolated females, and that this promotion is triggered by female-specific chemosensory signals (other than sex pheromone) primarily detected by antennae. Promotion of ootheca production between females is likely to be an early stage of social cooperation, reminiscent of the foundation and maintenance of a colony by female pairs in the eusocial termite Reticulitermes speratus.

9.
Sci Rep ; 5: 17195, 2015 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-26610608

RESUMO

Desiccate (Desi), initially discovered as a gene expressing in the epidermis of Drosophila larvae for protection from desiccation stress, was recently found to be robustly expressed in the adult labellum; however, the function, as well as precise expression sites, was unknown. Here, we found that Desi is expressed in two different types of non-neuronal cells of the labellum, the epidermis and thecogen accessory cells. Labellar Desi expression was significantly elevated under arid conditions, accompanied by an increase in water ingestion by adults. Desi overexpression also promoted water ingestion. In contrast, a knockdown of Desi expression reduced feeding as well as water ingestion due to a drastic decrease in the gustatory sensillar sensitivity for all tested tastants. These results indicate that Desi helps protect insects from desiccation damage by not only preventing dehydration through the integument but also accelerating water ingestion via elevated taste sensitivities of the sensilla.


Assuntos
Células Quimiorreceptoras/metabolismo , Desidratação/genética , Ingestão de Líquidos/genética , Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Proteínas de Membrana/genética , Sensilas/metabolismo , Animais , Células Quimiorreceptoras/ultraestrutura , Desidratação/metabolismo , Proteínas de Drosophila/antagonistas & inibidores , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Ingestão de Alimentos/genética , Regulação da Expressão Gênica , Larva/citologia , Larva/metabolismo , Masculino , Proteínas de Membrana/antagonistas & inibidores , Proteínas de Membrana/metabolismo , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Sensilas/ultraestrutura , Paladar/genética , Água/metabolismo
10.
J Invertebr Pathol ; 132: 190-200, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26519623

RESUMO

In this study, we characterized prophenoloxidase (proPO, (PPO)) genes of Tribolium castaneum and examined their involvement in antimicrobial host defense. Amino acid sequence comparison with well-characterized PPO proteins from other insect species suggested that T. castaneum PPO genes encoded functional proenzymes, with crucial sequence motifs being conserved. Developmental kinetics of the mRNA of two PPO genes, PPO1 and PPO2 in the pupal stage were different to each other. The PPO1 mRNA levels consistently decreased during pupal development while that of PPO2 peaked at mid-pupal stage. The two mRNAs also exhibited distinct responses upon immune challenges with heat-killed model microbes. The PPO1 mRNA stayed nearly unchanged by 6h post challenge, and was somewhat elevated at 24h. In contrast, the PPO2 mRNA significantly decreased at 3, 6 and 24h post challenge. These trends exhibited by respective PPO genes were consistent irrespective of the microbial species used as elicitors. Finally, we investigated the involvement of T. castaneum PPO genes in antimicrobial host defense by utilizing RNA interference-mediated gene silencing. Survival assays demonstrated that double knockdown of PPO genes, which was accompanied by weakened hemolymph PO activities, significantly impaired the host defense against Bacillus subtilis. By contrast, the knockdown did not influence the induction of any of the T. castaneum antimicrobial peptide genes that were studied here, except for one belonging to the gene group that shows very weak or negligible microbial induction. PPO knockdown as well weakened host defense against Beauveria bassiana moderately but significantly depending on the combination of infection methods and targeted genes. Our results indicated that the PPO genes represented constituents of both antibacterial and antifungal host defense of T. castaneum.


Assuntos
Catecol Oxidase/fisiologia , Besouros/imunologia , Precursores Enzimáticos/fisiologia , Interações Hospedeiro-Patógeno , Proteínas de Insetos/fisiologia , Motivos de Aminoácidos , Animais , Bacillus subtilis/imunologia , Catecol Oxidase/genética , Catecol Oxidase/metabolismo , Besouros/genética , Besouros/microbiologia , Precursores Enzimáticos/genética , Precursores Enzimáticos/metabolismo , Técnicas de Silenciamento de Genes , Hemolinfa/enzimologia , Imunidade Inata , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Larva/genética , Larva/imunologia , Larva/microbiologia , RNA Mensageiro/metabolismo , Análise de Sequência de Proteína
11.
Plant Physiol ; 167(3): 660-70, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25583926

RESUMO

Chlorophyllase (CLH) is a common plant enzyme that catalyzes the hydrolysis of chlorophyll to form chlorophyllide, a more hydrophilic derivative. For more than a century, the biological role of CLH has been controversial, although this enzyme has been often considered to catalyze chlorophyll catabolism during stress-induced chlorophyll breakdown. In this study, we found that the absence of CLH does not affect chlorophyll breakdown in intact leaf tissue in the absence or the presence of methyl-jasmonate, which is known to enhance stress-induced chlorophyll breakdown. Fractionation of cellular membranes shows that Arabidopsis (Arabidopsis thaliana) CLH is located in the endoplasmic reticulum and the tonoplast of intact plant cells. These results indicate that CLH is not involved in endogenous chlorophyll catabolism. Instead, we found that CLH promotes chlorophyllide formation upon disruption of leaf cells, or when it is artificially mistargeted to the chloroplast. These results indicate that CLH is responsible for chlorophyllide formation after the collapse of cells, which led us to hypothesize that chlorophyllide formation might be a process of defense against chewing herbivores. We found that Arabidopsis leaves with genetically enhanced CLH activity exhibit toxicity when fed to Spodoptera litura larvae, an insect herbivore. In addition, purified chlorophyllide partially suppresses the growth of the larvae. Taken together, these results support the presence of a unique binary defense system against insect herbivores involving chlorophyll and CLH. Potential mechanisms of chlorophyllide action for defense are discussed.


Assuntos
Arabidopsis/enzimologia , Arabidopsis/imunologia , Hidrolases de Éster Carboxílico/metabolismo , Herbivoria , Mastigação , Acetatos/farmacologia , Animais , Arabidopsis/efeitos dos fármacos , Arabidopsis/parasitologia , Bombyx/fisiologia , Clorofila/química , Clorofila/metabolismo , Clorofilídeos/metabolismo , Ciclopentanos/farmacologia , Retículo Endoplasmático/efeitos dos fármacos , Retículo Endoplasmático/metabolismo , Trato Gastrointestinal/metabolismo , Herbivoria/efeitos dos fármacos , Larva/fisiologia , Mutação , Oxilipinas/farmacologia , Fotossíntese/efeitos dos fármacos , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/parasitologia , Transporte Proteico/efeitos dos fármacos , Spodoptera/fisiologia , Frações Subcelulares/efeitos dos fármacos , Frações Subcelulares/metabolismo , Vacúolos/efeitos dos fármacos , Vacúolos/metabolismo
12.
Arch Insect Biochem Physiol ; 86(4): 220-39, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24986040

RESUMO

Lepidopteran larvae show a cellular response to invading foreign substances that are larger than hemocytes, for example, parasitoid eggs or larvae. This response is called hemocyte encapsulation and is often accompanied by phenoloxidase (PO)-catalyzed melanization. In the present study, we artificially transplanted endoparasitoid larvae and small glass fragments into the hemocoel of the common armyworm, Mythimna separata. We observed that the host larva showed a cellular response and that, 2-4 h after transplantation, melanin formation was spatially confined to the surface of the encapsulated substances. We further noted that specific morphological hemocytes surrounded by melanin formation became attached to the surface of the foreign substances. We designated these hemocytes hyperspread cells (HSCs) on the basis of their specific characteristics and circumferential spread. We confirmed the occurrence of prophenoloxidase (PPO)/phenoloxidase (PO) on the periphery of the HSCs and in the substance secreted around the HSCs by using anti-PPO antibody. We were unable to detect PPO-mRNA in HSCs by using in situ hybridization, although we showed that oenocytoids contained PPO-mRNA and PPO protein. We used light microscopy and scanning electron microscopy to discriminate five main types of circulating M. separata hemocytes. We observed that HSCs differed from plasmatocytes, but spread out well. Further, during the encapsulation process, HSCs appeared to provide a localized melanization spot on the surface of foreign invaders.


Assuntos
Hemócitos/imunologia , Imunidade Inata/imunologia , Melaninas/imunologia , Mariposas/imunologia , Animais , Catecol Oxidase/metabolismo , Eletroforese em Gel de Poliacrilamida , Precursores Enzimáticos/metabolismo , Hemócitos/ultraestrutura , Immunoblotting , Imuno-Histoquímica , Hibridização In Situ , Larva/imunologia , Melaninas/biossíntese , Microscopia Eletrônica de Varredura , Oligonucleotídeos/genética , Imagem com Lapso de Tempo
13.
J Invertebr Pathol ; 115: 26-32, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24184953

RESUMO

Polydnaviruses (PDVs) are unique symbiotic viruses associated with parasitoid wasps: PDV particles are injected into lepidopteran hosts along with the wasp eggs and express genes that interfere with aspects of host physiology such as immune defenses and development. Recent comparative genomic studies of PDVs have significantly improved our understanding of their origin as well as the genome organization. However, the structural features of functional PDV particles remain ambiguous. To clear up the structure of Cotesia kariyai PDV (CkPDV) particles, we focused on immunoevasive protein (IEP), which is a mediator of immunoevasion by the wasp from the encapsulation reaction of the host insect's hemocytes, because it has been demonstrated to be present on the surface of the virus particle. We discovered that IEP tends to polymerize and constitutes a previously unidentified thin surface layer covering CkPDV particles. This outermost surface layer looked fragile and was easily removed from CkPVD particles by mechanical stressors such as shaking, which prevented CkPDV from expressing the encoded genes in the host target tissues such as fat body or hemocytes. Furthermore, we detected IEP homologue gene expression in the wasp's venom reservoirs, implying IEP has another unknown biological function in the wasp or parasitized hosts. Taken together, the present results demonstrated that female C. kariyai wasps produce the fragile thin layer partly composed of IEP to cover the outer surfaces of CkPDV particles; otherwise, they cannot function as infectious agents in the wasp's host. The fact that IEP family proteins are expressed in both venom reservoirs and oviducts suggests an intimate relationship between both tissues in the development of the parasitism strategy of the wasp.


Assuntos
Interações Hospedeiro-Parasita/fisiologia , Polydnaviridae/patogenicidade , Vespas/virologia , Animais , Western Blotting , Cromatografia Líquida de Alta Pressão , Proteínas de Insetos/imunologia , Proteínas de Insetos/metabolismo , Polydnaviridae/imunologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Vírion/imunologia , Vírion/metabolismo , Vespas/imunologia , Vespas/metabolismo
14.
Protein Eng Des Sel ; 25(8): 405-13, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22706764

RESUMO

Silkworm ß-1,3-glucan recognition protein (ßGRP) tightly and specifically associates with ß-1,3-glucan. We report here an affinity purification system named the 'GRP system', which uses the association between the ß-1,3-glucan recognition domain of ßGRP (GRP-tag), as an affinity tag, and curdlan beads. Curdlan is a water-insoluble ß-1,3-glucan reagent, the low cost of which (about 100 JPY/g) allows the economical preparation of beads. Curdlan beads can be readily prepared by solubilization in an alkaline solution, followed by neutralization, sonication and centrifugation. We applied the GRP system to preparation of several proteins and revealed that the expression levels of the GRP-tagged proteins in soluble fractions were two or three times higher than those of the glutathione S-transferase (GST)-tagged proteins. The purity of the GRP-tagged proteins on the curdlan beads was comparable to that of the GST-tagged proteins on glutathione beads. The chemical stability of the GRP system was more robust than conventional affinity systems under various conditions, including low pH (4-6). Biochemical and structural analyses revealed that proteins produced using the GRP system were structurally and functionally active. Thus, the GRP system is suitable for both the large- and small-scale preparation of recombinant proteins for functional and structural analyses.


Assuntos
Proteínas de Transporte/química , Cromatografia de Afinidade/métodos , Proteínas de Insetos/química , Proteínas Recombinantes de Fusão/isolamento & purificação , beta-Glucanas/química , Sequência de Aminoácidos , Animais , Sequência de Bases , Bombyx/genética , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Proteína DEAD-box 58 , RNA Helicases DEAD-box/química , RNA Helicases DEAD-box/genética , RNA Helicases DEAD-box/metabolismo , Escherichia coli/genética , Glutationa Transferase/química , Glutationa Transferase/genética , Glutationa Transferase/metabolismo , Humanos , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Dados de Sequência Molecular , Estabilidade Proteica , Receptores Imunológicos , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , beta-Glucanas/metabolismo
15.
Sci Rep ; 2: 210, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22355724

RESUMO

Antimicrobial peptides (AMPs), major innate immune effectors, are induced to protect hosts against invading microorganisms. AMPs are also induced under non-infectious stress; however, the signaling pathways of non-infectious stress-induced AMP expression are yet unclear. We demonstrated that growth-blocking peptide (GBP) is a potent cytokine that regulates stressor-induced AMP expression in insects.GBP overexpression in Drosophila elevated expression of AMPs.GBP-induced AMP expression did not require Toll and immune deficiency (Imd) pathway-related genes, but imd and basket were essential,indicating that GBP signaling in Drosophila did not use the orthodox Toll or Imd pathway but used the JNK pathway after association with the adaptor protein Imd. The enhancement of AMP expression by non-infectious physical or environmental stressors was apparent in controls but not in GBP-knockdown larvae. These results indicate that the Drosophila GBP signaling pathway mediates acute innate immune reactions under various stresses, regardless of whether they are infectious or non-infectious.


Assuntos
Citocinas/farmacologia , Proteínas de Drosophila/farmacologia , Drosophila/metabolismo , Imunidade Inata/fisiologia , Estresse Fisiológico , Sequência de Aminoácidos , Animais , Animais Geneticamente Modificados , Sequência de Bases , Citocinas/química , Primers do DNA , Drosophila/imunologia , Proteínas de Drosophila/química , Dados de Sequência Molecular , Homologia de Sequência de Aminoácidos
16.
Insect Biochem Mol Biol ; 42(3): 183-92, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22198334

RESUMO

Growth rates of immature animals are governed by their feeding activities. A reduction in feeding sometimes causes serious growth retardation in insects; a typical case is often seen in host insects parasitized by a solitary endoparasitoid wasp. However, understanding of the mechanisms underlying the physiological repression of parasitized insects is fragmentary. Here we analyzed brain gene expression of the host common cutworm, Spodoptera litura, parasitized by a solitary endoparasitoid, Microplitis manilae, and identified a novel gene whose expression was significantly enhanced by parasitization. The gene encoded a pre-pro-peptide of a cytokine-like molecule and its expression was observed mainly in nervous tissues, hemocytes, and integuments. The 25 amino acid cytokine-like peptide encoded by the C-terminus of this gene was demonstrated to exist in the hemolymph of S. litura larvae and to change hemocytes from non-adhesive to adhesive in vitro. Further, injection of the active peptide reduced feeding activities of test larvae and consequently delayed their growth. The enhanced gene expression was also observed in larvae under severe stress conditions: abdominal ligature, proleg cutting, mechanical vibration, low temperature, and heat shock at 45°C. Elevated gene expression was maintained only in seriously growth-retarded larvae but not in recovered larvae at 24h or 48h after heat treatment. Thus, it is reasonable to conclude that stress-induced elevation of the peptide gene expression highly correlates with reduced feeding activities and growth retardation of the host larvae parasitized by M. manilae. Based on the conclusion, we named this peptide stress-responsive peptide (SRP).


Assuntos
Proteínas de Insetos/genética , Spodoptera/fisiologia , Estresse Fisiológico , Sequência de Aminoácidos , Animais , Sequência de Bases , Proteínas de Insetos/metabolismo , Larva/crescimento & desenvolvimento , Larva/metabolismo , Dados de Sequência Molecular , Spodoptera/parasitologia , Vespas/fisiologia
18.
Proc Natl Acad Sci U S A ; 107(36): 15862-7, 2010 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-20798052

RESUMO

Growth-blocking peptide (GBP) is an insect cytokine that stimulates a class of immune cells called plasmatocytes to adhere to one another and to foreign surfaces. Although extensive structure-activity studies have been performed on the GBP and its mutants in Lepidoptera Pseudaletia separata, the signaling pathway of GBP-dependent activation of plasmatocytes remains unknown. We identified an adaptor protein (P77) with a molecular mass of 77 kDa containing SH2/SH3 domain binding motifs and an immunoreceptor tyrosine-based activation motif (ITAM)-like domain in the cytoplasmic region of the C terminus. Although P77 showed no capacity for direct binding with GBP, its cytoplasmic tyrosine residues were specifically phosphorylated within seconds after GBP was added to a plasmatocyte suspension. Tyrosine phosphorylation of P77 also was observed when hemocytes were incubated with Enterobactor cloacae or Micrococcus luteus, but this phosphorylation was found to be induced by GBP released from hemocytes stimulated by the pathogens. Tyrosine phosphorylation of the integrin beta subunit also was detected in plasmatocytes stimulated by GBP. Double-stranded RNAs targeting P77 not only decreased GBP-dependent tyrosine phosphorylation of the integrin beta subunit, but also abolished GBP-induced spreading of plasmatocytes on foreign surfaces. P77 RNAi larvae also showed significantly higher mortality than control larvae after infection with Serratia marcescens, indicating that P77 is essential for GBP to mediate a normal innate cellular immunity in insects. These results demonstrate that GBP signaling in plasmatocytes requires the adaptor protein P77, and that active P77-assisted tyrosine phosphorylation of integrins is critical for the activation of plasmatocytes.


Assuntos
Citocinas/metabolismo , Hemócitos/metabolismo , Proteínas de Insetos/fisiologia , Insetos/metabolismo , Transdução de Sinais , Sequência de Aminoácidos , Animais , Proteínas de Insetos/química , Proteínas de Insetos/metabolismo , Dados de Sequência Molecular
19.
Proc Natl Acad Sci U S A ; 106(28): 11679-84, 2009 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-19561300

RESUMO

The beta-1,3-glucan recognition protein (betaGRP)/Gram-negative bacteria-binding protein 3 (GNBP3) is a crucial pattern-recognition receptor that specifically binds beta-1,3-glucan, a component of fungal cell walls. It evokes innate immunity against fungi through activation of the prophenoloxidase (proPO) cascade and Toll pathway in invertebrates. The betaGRP consists of an N-terminal beta-1,3-glucan-recognition domain and a C-terminal glucanase-like domain, with the former reported to be responsible for the proPO cascade activation. This report shows the solution structure of the N-terminal beta-1,3-glucan recognition domain of silkworm betaGRP. Although the N-terminal domain of betaGRP has a beta-sandwich fold, often seen in carbohydrate-binding modules, both NMR titration experiments and mutational analysis showed that betaGRP has a binding mechanism which is distinct from those observed in previously reported carbohydarate-binding domains. Our results suggest that betaGRP is a beta-1,3-glucan-recognition protein that specifically recognizes a triple-helical structure of beta-1,3-glucan.


Assuntos
Bombyx/genética , Proteínas de Transporte/genética , Imunidade Inata/genética , Proteínas de Insetos/genética , Modelos Moleculares , Ligação Proteica , Sequência de Aminoácidos , Animais , Sequência de Bases , Bombyx/imunologia , Proteínas de Transporte/metabolismo , Análise Mutacional de DNA , Proteínas de Insetos/metabolismo , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Conformação Proteica , Dobramento de Proteína , Alinhamento de Sequência , beta-Glucanas/metabolismo
20.
Dev Cell ; 10(1): 45-55, 2006 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16399077

RESUMO

The Toll receptor was originally identified as an indispensable molecule for Drosophila embryonic development and subsequently as an essential component of innate immunity from insects to humans. Although in Drosophila the Easter protease processes the pro-Spätzle protein to generate the Toll ligand during development, the identification of the protease responsible for pro-Spätzle processing during the immune response has remained elusive for a decade. Here, we report a protease, called Spätzle-processing enzyme (SPE), required for Toll-dependent antimicrobial response. Flies with reduced SPE expression show no noticeable pro-Spätzle processing and become highly susceptible to microbial infection. Furthermore, activated SPE can rescue ventral and lateral development in embryos lacking Easter, showing the functional homology between SPE and Easter. These results imply that a single ligand/receptor-mediated signaling event can be utilized for different biological processes, such as immunity and development, by recruiting similar ligand-processing proteases with distinct activation modes.


Assuntos
Proteínas de Drosophila/metabolismo , Imunidade/fisiologia , Serina Endopeptidases/fisiologia , Transdução de Sinais/fisiologia , Receptores Toll-Like/metabolismo , Animais , Animais Geneticamente Modificados , Linhagem Celular , Drosophila/imunologia , Proteínas de Drosophila/deficiência , Embrião não Mamífero/metabolismo , Indução Embrionária , Ativação Enzimática , Corpo Adiposo/imunologia , Regulação da Expressão Gênica no Desenvolvimento , Modelos Biológicos , Dados de Sequência Molecular , Ligação Proteica/fisiologia , Estrutura Secundária de Proteína , RNA Mensageiro/biossíntese , Proteínas Recombinantes/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Homologia de Sequência de Aminoácidos , Fatores de Tempo
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