RESUMO
Trichinellosis, a zoonotic disease caused by nematodes of the genus Trichinella, is still a public health concern in the Arctic. The aims of this study were to investigate the seroprevalence of anti-Trichinella IgG in aboriginal peoples of two settlements in the Chukotka Autonomous Okrug (Russian Federation) on the Arctic coast of the Bering Sea, and to evaluate the survival of Trichinella nativa larvae in local fermented and frozen meat products. A seroprevalence of 24.3% was detected in 259 people tested by an enzyme-linked immunosorbent assay (ELISA). The highest prevalence was detected among people who consumed traditional local foods made from the meat of marine mammals. Trichinella nativa larvae were found to survive for up to 24 months in a fermented and frozen marine mammal meat product called kopalkhen. Since the T. nativa life cycle can be completed in the absence of humans, it can be expected to persist in the environment and therefore remain a cause of morbidity in the human populations living in Arctic regions.
Assuntos
Alimentos Congelados/parasitologia , Carne/parasitologia , Triquinelose/epidemiologia , Animais , Anticorpos Anti-Helmínticos/sangue , Organismos Aquáticos/parasitologia , Regiões Árticas/epidemiologia , Ensaio de Imunoadsorção Enzimática , Humanos , Imunoglobulina G/sangue , Larva/fisiologia , Prevalência , Federação Russa/epidemiologia , Estudos Soroepidemiológicos , Trichinella/imunologia , Triquinelose/etnologiaAssuntos
DNA de Protozoário/genética , Modelos Animais de Doenças , Giardia lamblia/crescimento & desenvolvimento , Giardíase/parasitologia , Estágios do Ciclo de Vida/fisiologia , Animais , Animais não Endogâmicos , Fezes/parasitologia , Giardia lamblia/classificação , Giardia lamblia/genética , Giardíase/patologia , Humanos , Intestinos/parasitologia , Intestinos/patologia , Reação em Cadeia da Polimerase , Ratos , Fatores de TempoRESUMO
Our first experience in genotyping Giardia from Moscow residents, has shown that 4 and 2 of seven samples belong to G. duodenalis genotype A and genotype B, respectively; one sample was negative during amplification with two types of primers. Genotyping was Carried out using the specific primers TPIA and TPIB to the gene encoding for the enzyme triosephosphate isomerase from the parasite. Thus, further such investigations using a larger number of samples will be able to complement the epidemiology of Lamblia infection in Moscow residents.
