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1.
Rev Sci Instrum ; 89(4): 043706, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29716370

RESUMO

For many scientific questions gaining three-dimensional insight into a specimen can provide valuable information. We here present an instrument called "tOMography Nano crYo (OMNY)," dedicated to high resolution 3D scanning x-ray microscopy at cryogenic conditions via hard X-ray ptychography. Ptychography is a lens-less imaging method requiring accurate sample positioning. In OMNY, this in achieved via dedicated laser interferometry and closed-loop position control reaching sub-10 nm positioning accuracy. Cryogenic sample conditions are maintained via conductive cooling. 90 K can be reached when using liquid nitrogen as coolant, and 10 K is possible with liquid helium. A cryogenic sample-change mechanism permits measurements of cryogenically fixed specimens. We compare images obtained with OMNY with older measurements performed using a nitrogen gas cryo-jet of stained, epoxy-embedded retina tissue and of frozen-hydrated Chlamydomonas cells.


Assuntos
Imageamento Tridimensional/instrumentação , Microscopia/instrumentação , Tomografia por Raios X/instrumentação , Animais , Chlamydomonas , Criopreservação/instrumentação , Desenho de Equipamento , Interferometria/instrumentação , Lasers , Imagem Óptica/instrumentação , Retina/citologia , Espalhamento a Baixo Ângulo , Temperatura , Difração de Raios X/instrumentação
2.
Opt Express ; 24(18): 20245-52, 2016 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-27607631

RESUMO

Ptychographic Coherent diffractive imaging (PCDI) is a significant advance in imaging allowing the measurement of the full electric field at a sample without use of any imaging optics. So far it has been confined solely to imaging of linear optical responses. In this paper we show that because of the coherence-preserving nature of nonlinear optical interactions, PCDI can be generalised to nonlinear optical imaging. We demonstrate second harmonic generation PCDI, directly revealing phase information about the nonlinear coefficients, and showing the general applicability of PCDI to nonlinear interactions.

3.
Opt Express ; 24(8): 8360-9, 2016 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-27137273

RESUMO

Ptychography is a scanning coherent diffractive imaging (CDI) technique that relies upon a high level of stability of the illumination during the course of an experiment. This is particularly an issue for coherent short wavelength sources, where the beam intensity is usually tightly focused on the sample in order to maximize the photon flux density on the illuminated region of the sample and thus a small change in the beam position results in a significant change in illumination of the sample. We present an improved ptychographic method that allows for limited stability of the illumination wavefront and thus significantly improve the reconstruction quality without additional prior knowledge. We have tested our reconstruction method in a proof of concept experiment, where the beam instability of a visible light source was emulated using a piezo driven mirror, and also in a short wavelength microscopy CDI setup using a high harmonic generation source in the extreme ultraviolet range. Our work shows a natural extension of the ptychography method that paves the way to use ptychographic imaging with any limited pointing stability coherent source such as free electron or soft X-ray lasers and improve reconstruction quality of long duration synchrotron experiments.

4.
Rev Sci Instrum ; 87(12): 123505, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28040918

RESUMO

The soft X-ray (SXR) emission provides valuable insight into processes happening inside of high-temperature plasmas. A standard method for deriving the local emissivity profiles of the plasma from the line-of-sight integrals measured by pinhole cameras is the tomographic inversion. Such an inversion is challenging due to its ill-conditioned nature and because the reconstructed profiles depend not only on the quality of the measurements but also on the inversion algorithm used. This paper provides a detailed description of several tomography algorithms, which solve the inversion problem of Tikhonov regularization with linear computational complexity in the number of basis functions. The feasibility of combining these methods with the minimum Fisher information regularization is demonstrated, and various statistical methods for the optimal choice of the regularization parameter are investigated with emphasis on their reliability and robustness. Finally, the accuracy and the capability of the methods are demonstrated by reconstructions of experimental SXR profiles, featuring poloidal asymmetric impurity distributions as measured at the ASDEX Upgrade tokamak.

5.
Opt Lett ; 40(23): 5574-7, 2015 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-26625054

RESUMO

We report the demonstration of a scanning probe coherent diffractive imaging method (also known as ptychographic CDI) using a compact and partially coherent gas-discharge plasma source of extreme ultraviolet (EUV) radiation at a 17.3 nm wavelength. Until now, CDI has been mainly carried out with coherent, high-brightness light sources, such as third generation synchrotrons, x-ray free-electron lasers, and high harmonic generation. Here we performed ptychographic lensless imaging of an extended sample using a compact, lab-scale source. The CDI reconstructions were achieved by applying constraint relaxation to the CDI algorithm. Experimental results indicate that our method can handle the low spatial coherence and broadband nature of the EUV illumination, as well as the residual background due to visible light emitted by the gas-discharge source. The ability to conduct ptychographic imaging with lab-scale and partially coherent EUV sources is expected to significantly expand the applications of this powerful CDI method.

6.
Rev Sci Instrum ; 85(11): 11E433, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25430340

RESUMO

The soft x-ray diagnostic is suitable for monitoring plasma activity in the tokamak core, e.g., sawtooth instability. Moreover, spatially resolved measurements can provide information about plasma position and shape, which can supplement magnetic measurements. In this contribution, fast algorithms with the potential for a real-time use are tested on the data from the COMPASS tokamak. In addition, the soft x-ray data are compared with data from other diagnostics in order to discuss possible connection between sawtooth instability on one side and the transition to higher confinement mode, edge localized modes and productions of runaway electrons on the other side.

7.
Rev Sci Instrum ; 85(1): 013509, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24517767

RESUMO

Light reflections are one of the main and often underestimated issues of plasma emissivity reconstruction in visible light spectral range. Metallic and other specular components of tokamak generate systematic errors in the optical measurements that could lead to wrong interpretation of data. Our analysis is performed at data from the tokamak COMPASS. It is a D-shaped tokamak with specular metallic vessel and possibility of the H-mode plasma. Data from fast visible light camera were used for tomographic reconstruction with background reflections subtraction to study plasma boundary. In this article, we show that despite highly specular tokamak wall, it is possible to obtain a realistic reconstruction. The developed algorithm shows robust results despite of systematic errors in the optical measurements and calibration. The motivation is to obtain an independent estimate of the plasma boundary shape.

8.
Rev Sci Instrum ; 83(10): 10E505, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23127012

RESUMO

We present design, analysis, and performance evaluation of a new, low cost and high speed visible-light camera diagnostic system for tokamak experiments. The system is based on the camera Casio EX-F1, with the overall price of approximately a thousand USD. The achieved temporal resolution is up to 40 kHz. This new diagnostic was successfully implemented and tested at the university tokamak GOLEM (R = 0.4 m, a = 0.085 m, B(T) < 0.5 T, I(p) < 4 kA). One possible application of this new diagnostic at GOLEM is discussed in detail. This application is tomographic reconstruction for estimation of plasma position and emissivity.

9.
Rev Sci Instrum ; 83(10): 10E531, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23130804

RESUMO

The contribution focuses on plasma tomography via the minimum Fisher regularisation (MFR) algorithm applied on data from the recently commissioned tomographic diagnostics on the COMPASS tokamak. The MFR expertise is based on previous applications at Joint European Torus (JET), as exemplified in a new case study of the plasma position analyses based on JET soft x-ray (SXR) tomographic reconstruction. Subsequent application of the MFR algorithm on COMPASS data from cameras with absolute extreme ultraviolet (AXUV) photodiodes disclosed a peaked radiating region near the limiter. Moreover, its time evolution indicates transient plasma edge cooling following a radial plasma shift. In the SXR data, MFR demonstrated that a high resolution plasma positioning independent of the magnetic diagnostics would be possible provided that a proper calibration of the cameras on an x-ray source is undertaken.

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