RESUMO
Fast, simple and accurate methods for the determination of SDZ ICM 567, the 7-methoxy derivative of tropisetron, in microdialysates have been developed. Sampling by microdialysis from freely moving rats in the portal and jugular vein offers a new technology for pharmacokinetic studies by direct and continuous measurement of unbound drug concentrations with time. SDZ ICM 567 can be identified in small sample volumes of dialysates on a microbore high-performance liquid chromatography column-switching system with ultraviolet detection. In addition, determination of SDZ ICM 567 by fluorimetric detection has been developed for muscle microdialysates from rats. [14C]SDZ ICM 567 was used as reference substance for the estimation of the amount of substance transferred through the dialysis membrane. The radioactive measurement (RA) gave the recovery information, whereas the liquid chromatographic method detected the sum of [14C]SDZ ICM 567 and dialyzed SDZ ICM 567.
Assuntos
Ansiolíticos/sangue , Compostos Bicíclicos Heterocíclicos com Pontes/sangue , Soluções para Diálise/análise , Indóis/sangue , Músculos Peitorais/química , Administração Oral , Animais , Ansiolíticos/administração & dosagem , Ansiolíticos/análise , Ansiolíticos/farmacocinética , Compostos Bicíclicos Heterocíclicos com Pontes/administração & dosagem , Compostos Bicíclicos Heterocíclicos com Pontes/análise , Compostos Bicíclicos Heterocíclicos com Pontes/farmacocinética , Cromatografia Líquida , Indóis/administração & dosagem , Indóis/análise , Indóis/farmacocinética , Injeções Intravenosas , Masculino , Microdiálise/métodos , Ratos , Ratos Wistar , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta , VeiasAssuntos
Compostos Bicíclicos Heterocíclicos com Pontes/farmacocinética , Indóis/farmacocinética , Músculos/metabolismo , Antagonistas da Serotonina/farmacocinética , Animais , Compostos Bicíclicos Heterocíclicos com Pontes/administração & dosagem , Compostos Bicíclicos Heterocíclicos com Pontes/sangue , Difusão , Indóis/administração & dosagem , Indóis/sangue , Infusões Intravenosas , Masculino , Microdiálise , Ratos , Ratos Wistar , Antagonistas da Serotonina/administração & dosagem , Antagonistas da Serotonina/sangueRESUMO
A liquid chromatographic method for the determination of pyrethrins and of the synergist piperonyl butoxide in human plasma after C18 solid-phase extraction is described. UV detection was found to be sensitive enough to determine concentrations far below the limit of toxicity. With respect to future investigations concerning studies in biological materials, a column-switching system for sample preparation was developed and compared with solid-phase extraction. Both methods show comparable limits of detection, but the column-switching technique has the advantage of fully automating the system.
