RESUMO
A simple reversed-phase liquid chromatographic method has been developed to determine protease inhibitors concentrations in plasma. Plasma samples (250 micro l) containing protease inhibitors were prepared by a simple deproteinization (recovery: 92, 91, 91 and 90.5% for ritonavir, saquinavir, nelfinavir and M8 nelfinavir metabolite, respectively). Chromatography was accomplished using a Hypersil octadecylsilyl column (100 x 4.6 mm I.D.) and a mobile phase composed of acetonitrile, tetrahydrofuran and dihydrogenophosphate buffer (pH 4) (32:10:58, v/v). Ultraviolet detection at 210 nm was used. The limit of detection was 200 ng/ml for ritonavir, saquinavir, nelfinavir and M8 nelfinavir metabolite. Calibration curves were linear up to 20000 ng/ml, with correlation coefficients better than 0.997 for all compounds. Intra- and inter-day coefficients of variation of the assay were Assuntos
Cromatografia Líquida de Alta Pressão/métodos
, Inibidores da Protease de HIV/sangue
, Nelfinavir/sangue
, Ritonavir/sangue
, Saquinavir/sangue
, Inibidores da Protease de HIV/farmacocinética
, Humanos
, Nelfinavir/farmacocinética
, Padrões de Referência
, Ritonavir/farmacocinética
, Saquinavir/farmacocinética
, Sensibilidade e Especificidade