[A fluorometric method of determining DNA in cells]. / Fluorometricheskii metod opredeleniia DNK v kletkakh.

A convenient express method is proposed for cell lysis and DNA solubilization in the presence of sarcosyl and sodium citrate. The DNA content was determined in cell lysates by registration of fluorescence enhancement with Hoechst No 33258 ("Serva", FRG). In 0.5 M NaCl solution the Hoechst-RNA fluorescence is negligible. An optimal molar ratio of DNA and Hoechst is in the range from 0.2 to 2.0. Beyond the range the accuracy of DNA quantification become poor. An optimal range of DNA quantification in cells is 200-2000 ng/ml.

[Change in concentration of microelements in denervated muscles]. / Izmenenie soderzhaniia mikroélementov v myshtsakh pri denervatsii.

Concentration of Zn, Br, and Rb in intact and denervated rat muscles was estimated by X-ray fluorescent analysis. Differences in the content of these elements at different times after denervation were found. Concentration of microelements (microgram/g dry weight) in denervated muscles was shown to increase by the end of the third week, while that in the intact muscle decreased. The analysis of microelement content in the intact muscle revealed two steps of postdenervational changes: hypertrophic and atrophic ones. Differences between the above changes in microelement concentrations and their content in the intact muscle suggest a hypothesis that protein dissociation and utilization of microelements in muscle cells after denervation proceeds with different rates.

[Difference in the content of trace elements in muscles]. / Razlichiia myshts po mikroelementnomu sostavu.

By using X-ray fluorescence analysis we have performed a comparative study of the trace elements content of phasic and tonic muscles of scallops. The phasic (yellow) muscles were found to be richer in Fe and Cu and more deficient in Zn, Br, and Sr, as compared with the tonic (white) muscles. No appreciable difference in the Rb content was revealed. An analysis of the results obtained allows one to outline a classification of Fe, Cu, Zn, Br and Sr according to their properties which are connected with various homeostatic requirements of the muscles with regard to the trace elements.

[Localization of adenine components in chick myofibrils by the ferritin antibody technic]. / Izuchenie lokalizatsii adeninovykh komponentov v miofibrillakh tsyplenka metodom ferritinovykh antitel.

The immunochemical method has been proposed to indicate the adenin compounds in isolated myofibrils of striated muscle. Adenosine--containing antigen was prepared by coupling adenosine with bovine serum albumin (adenosine--BSA). Antibodies against adenosine--BSA were labelled by ferritin. The sites of the antigen antibody reaction were marked by localization of ferritin molecules. Ferritin antibodies were found to concentrate in the following sarcomere sites: M and Z bands, N-lines and terminal cisternae of sarcoplasmic reticulum near the Z-bands.

[Protein evolution rate and immunoglobulin induction]. / Skorost' évoliutsii belkov i induktsiia immunoglobulinov.

The capacity of proteins to induce the synthesis of specific immunoglobulins was shown to be correlated with their evolution rate. This correlation can be understood in terms of the clonal-selectional theory. The characteristic correlation parameter is the number of differences in the amino acid sequences between the immunogenic protein and the homological protein of the immunized animal. The above correlation was traced most clearly for the evolutionary conservative proteins.