An Experimental and Numerical Study on the Use of Chirped FBG Sensors for Monitoring Fatigue Damage in Hybrid Composite Patch Repairs.

In this paper, chirped fibre Bragg grating (CFBG) sensors used to monitor the structural health of a composite patch used to repair an aluminium panel is presented. To introduce damage, a notch was produced at the centre of an aluminium panel. The repair consisted of bonding a pre-cured composite patch to the host panel using an aerospace-grade film adhesive; the sensor was embedded in the bond-line during fabrication of the repair. The repaired panels were subjected to tension-tension loading in fatigue. Cracks initiated and grew from both ends of the notch in the aluminium panels and the fatigue loading was stopped periodically for short periods of time to record the reflected spectra from the sensor. It was found that perturbations in the reflected spectra began to occur when the crack was within about 2 to 3 mm of the sensor location; after the crack passed the sensor location, the perturbations essentially stabilised. Predicted reflected spectra have been found to be in good agreement with the experiment, confirming that CFBG sensors can detect crack growth in patch-repaired panels.

Rapid, automated measurement of dielectrophoretic forces using DEP-activated microwells.

Dielectrophoresis (DEP) is a physical effect that generates a force on polarisable particles experiencing a non-homogeneous electric field; studying the effect as a function of frequency allows the determination of the electrical properties of that particle, i.e., the electrical permittivity and conductivity. In the past, DEP-based techniques applied to the measurement of one or several cells at a time have been subject to many sources of noise, which result in an ambiguous or inaccurate result. However, improvements are possible by generating more information from the experiments. In this paper, we present a rapid automated system that measures the DEP spectrum from a large population of cells with a low level of noise using the microwell electrodes, based on a method of analysis that provides additional information about the electrical properties of the cells and a new theoretical approach was developed to obtain accurate, bias-free results in <5 min.

Dielectrophoresis-activated multiwell plate for label-free high-throughput drug assessment.

Dielectrophoresis (DEP) offers many advantages over conventional cell assays such as flow cytometry and patch clamp techniques for assessing cell electrophysiology as a marker for cancer studies and drug interaction assessment. However, despite the advantages offered by DEP analysis, uptake has been low, remaining largely in the academic arena, due to the process of analysis being time-consuming, laborious, and ultimately allowing only serial analysis on small numbers of cells. In this paper we describe a new method of performing DEP analysis based on laminate manufacturing methods. These use a three-dimensional "well" structure, similar in size and pitch to conventional microtiter well plates, but offer electrodes along the inner surface to allow easy measurement of cell properties through the whole population. The result can then be determined rapidly using a conventional well-plate reader. The nature of the device means that many electrodes, each containing a separate sample, can be tested in parallel, while the mode of observation means that analysis can be combined with simultaneous measurement of conventional fluorimetric well-based assays. Here we benchmark the device against standard DEP assays, then show how such a device can be used to (a) rapidly determine the effects both of ion channel blockers on cancer cells and antibiotics on bacteria and (b) determine the properties of multiple subpopulations of cells within a well simultaneously.

A high-throughput 3-D composite dielectrophoretic separator.

Dielectrophoresis has great potential to offer a range of diverse fields from bioprocessing to clinical medicine, but is hampered by low throughput rates to the micrometer scale of the electrodes required to generate highly nonuniform fields. Here we describe a novel approach to electrode construction, using a drilled laminated structure to form channels bearing electrodes 30 microm across and 150 microm apart. Since these electrodes appear along all sides of the drilled bore, the trapping efficiency is improved over conventional devices. We have developed and demonstrated a separator capable of sorting a 50:50 mixture of viable and nonviable yeast cells into an 86:14 mixture at 25 mLhr(-1).