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1.
Biotech Histochem ; 92(1): 15-28, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28098485

RESUMO

The use of stem or progenitor cells from bone marrow, or peripheral or umbilical cord blood is becoming more common for treatment of diabetic foot problems. These cells promote neovascularization by angiogenic factors and they promote epithelium formation by stimulating cell replication and migration under certain pathological conditions. We investigated the role of CD34 + stem cells from human umbilical cord blood in wound healing using a rat model. Rats were randomly divided into a control group and two groups with diabetes induced by a single dose of 55 mg/kg intraperitoneal streptozocin. Scarred areas 5 mm in diameter were created on the feet of all rats. The diabetic rats constituted the diabetes control group and a diabetes + stem cell group with local injection into the wound site of 0.5 × 106 CD34 + stem cells from human umbilical cord blood. The newly formed skin in the foot wounds following CD34 + stem cell treatment showed significantly improvement by immunohistochemistry and TUNEL staining, and were closer to the wound healing of the control group than the untreated diabetic animals. The increase in FGF expression that accompanied the local injection of CD34 + stem cells indicates that FGF stimulation helped prevent apoptosis. Our findings suggest a promising new treatment approach to diabetic wound healing.


Assuntos
Antígenos CD34/farmacologia , Pé Diabético/tratamento farmacológico , Sangue Fetal/citologia , Transplante de Células-Tronco , Células-Tronco/fisiologia , Cicatrização , Animais , Antígenos CD34/administração & dosagem , Biomarcadores , Diabetes Mellitus Experimental , Regulação da Expressão Gênica , Humanos , Neovascularização Fisiológica , Distribuição Aleatória , Ratos
2.
Biotech Histochem ; 90(2): 132-9, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25314162

RESUMO

Aluminium has toxic effects on many organ systems of the human body. Aluminium toxicity also is a factor in many neurodegenerative diseases. We investigated changes in numbers of hippocampal neurons in rats exposed to aluminium using an optical fractionator and we investigated aluminium-induced apoptosis using the transferase mediated dUTP nick end labeling (TUNEL) assay. Twenty-four female rats were divided equally into control, sham and aluminium-exposed groups. The control group received no treatment. The two treatment groups were injected intraperitoneally with 1 ml 0.9% saline without (sham) and with 3 mg/ml aluminium sulfate every day for two weeks. Following the treatments, the brains were removed, the left hemisphere was used for hippocampal neuron counting using an optical fractionator and the right hemisphere was investigated using hippocampal TUNEL assay to determine the apoptotic index. The number of neurons in the stratum pyramidale of the hippocampus was significantly less in the aluminium group than in the control and sham groups; there was no significant difference between the control and sham groups. The apoptotic index also was significantly higher in the aluminium group than in the other two groups. We quantified the toxic effects of aluminium on the rat hippocampus and determined that apoptosis was the mechanism of aluminium-induced neuron death in the hippocampus.


Assuntos
Compostos de Alúmen/toxicidade , Apoptose/efeitos dos fármacos , Hipocampo/efeitos dos fármacos , Animais , Feminino , Hipocampo/citologia , Marcação In Situ das Extremidades Cortadas/métodos , Neurônios/citologia , Neurônios/efeitos dos fármacos , Ratos Wistar , Cloreto de Sódio
3.
Biotech Histochem ; 89(1): 23-8, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23848547

RESUMO

Copper sulfate can cause different pathologies in different organ systems during development. We determined the effects of toxic levels of copper sulfate on brain development in term Hubbard broiler chicks using stereological and biochemical analyses. Hubbard broiler chicken eggs were divided into three groups: controls with no treatment, sham-treated animals and an experimental group. On day 1, 0.1 ml saline was injected into the air chambers of the sham and experimental groups. The experimental group received also 50 µg copper sulfate. At term (day 21), all chick brains were removed and their volumes were determined using the Cavalieri volume estimation. Parallel biochemical analyses were carried out for glutathione and malondialdehyde levels in the brain tissues as indicators of oxidative damage. With copper treatment, the mean brain volume (8079 µm(3)) was significantly decreased compared to both the control (10075 µm(3)) and sham (9547 µm(3)) groups. Copper treatment (143.8 nmol/g tissue) showed significantly decreased malondialdehyde levels compared to the control (293.6 nmol/g tissue) and sham groups (268.8 nmol/g tissue). Copper treatment (404.5 nmol/g tissue) showed significantly increased malondialdehyde levels compared to the control (158.6 nmol/g tissue) and sham (142.8 nmol/g tissue) groups. The morphological and biochemical parameters we measured demonstrated that in term Hubbard broiler chicks, toxic levels of copper sulfate cause developmental and oxidative brain damage.


Assuntos
Encéfalo/efeitos dos fármacos , Galinhas/fisiologia , Sulfato de Cobre/toxicidade , Animais , Apoptose/efeitos dos fármacos , Química Encefálica/efeitos dos fármacos , Glutationa/análise , Malondialdeído/análise , Tamanho do Órgão/efeitos dos fármacos , Oxirredução , Estresse Oxidativo/efeitos dos fármacos
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