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Anal Bioanal Chem ; 408(22): 5963-71, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27173395

RESUMO

Recently, point of care testing (POCT) used for diagnosis of influenza infection has a problem showing false negative diagnosis because of the low sensitivity. We would like to report detection of influenza virus A (H1N1) by an immunosensor based on electrochemiluminescence (ECL) that uses an immunoliposome encapsulating tris(2,2'-bipyridyl)ruthenium(II) complex. By using the sensor, we could detect the virus that competed with hemagglutinin (HA) peptide immobilized on self-assembled monolayers (SAMs) in immunoreaction of the antibody bound on the surface of liposome. The HA peptide was 19 mer (TGLRNGITNKVNSVIEKAA). We demonstrated great improvement of sensitivity and accuracy by introducing binary SAMs instead of mono SAMs. The binary SAMs was prepared from 3,3'-dithiodipropionic acid and 1-hexanethiol. Use of the binary SAMs enabled to increase the SAMs coverage on Au electrode; the fact was confirmed by observation of the cathodic desorption currents. By using such an electrode, first the detection method of BSA was optimized to lower ECL background signal. Then we applied the method to the detection of influenza virus. We could successfully detect the virus with higher sensitivity compared with that by POCT and ELISA. The detection range was from a concentration of 2.7 × 10(2) to 2.7 × 10(3) PFU/mL.


Assuntos
Técnicas Biossensoriais/métodos , Vírus da Influenza A Subtipo H1N1/isolamento & purificação , Lipossomos/química , Medições Luminescentes/métodos , Compostos Organometálicos/química , Animais , Anticorpos Imobilizados/química , Bovinos , Hemaglutininas/química , Humanos , Proteínas Imobilizadas/química , Imunoensaio/métodos , Influenza Humana/diagnóstico , Influenza Humana/virologia , Peptídeos/química , Sensibilidade e Especificidade , Soroalbumina Bovina/análise
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