Assuntos
Lactamas/farmacologia , Infecções por Pseudomonas/tratamento farmacológico , Pseudomonas aeruginosa/enzimologia , Pseudomonas aeruginosa/genética , beta-Lactamases/genética , Contagem de Colônia Microbiana , Humanos , Japão , Testes de Sensibilidade Microbiana , Reação em Cadeia da Polimerase/métodos , Pseudomonas aeruginosa/efeitos dos fármacos , Escarro/microbiologia , Resistência beta-Lactâmica/genética , beta-Lactamases/isolamento & purificaçãoRESUMO
Here we report a rather simple and convenient chemiluminescence (CL) method for the determination of tiopronin. It was based that tiopronin could greatly enhance CL between H2O2 and luminol in a basic alkaline solution. Light emission is intense, and even with a simple setup a high sensitivity could be achieved. The linear range was 3 mM-500 nM with a detection limit of 200 nM. Singlet oxygen and hydroxyl radical were suggested to be produced in this reaction and was responsible for the CL of tiopronin. As a preliminary application, this simple method has been successfully applied into the determination of tiopronin in a pharmaceutical formulation.
Assuntos
Tiopronina/análise , Tiopronina/química , Química Farmacêutica , Medições Luminescentes , Preparações Farmacêuticas/análise , Preparações Farmacêuticas/químicaRESUMO
We present a sensitive method for the determination of cefaclor (CCL), a beta-lactam antibiotic. It was based on the chemical derivatization of the drug with 4-(2'-cyanoisoindolyl)phenylisothiocynate (CIPIC) under the reaction conditions with heating at 80 degrees C for 7 min in the presence of pyridine. The CIPIC reagent could react with the primary amino group of the drug to form the CIPIC-conjugated CCL. The derivatives emitted not only fluorescence (FL) at maximum emission wavelength of 410 nm with irradiation at 310 nm, but also chemiluminescence (CL) in the presence of H(2)O(2), borate buffer (pH 9.6) and acetonitrile. After separation of the CIPIC derivatives of CCL and cephradine as internal standard in human serum by reversed-phase liquid chromatography, the derivatives could be monitored with both FL and CL detections. The detection limit (S/N=3) in the chromatograph was 1 pmol by the CL detection and 10 pmol by the FL detection. The proposed CL method permitted the most sensitive determination of CCL in the human serum after its oral administration.
Assuntos
Antibacterianos/análise , Cefaclor/análise , Tecnologia Farmacêutica/métodos , Adulto , Antibacterianos/química , Cefaclor/química , Cromatografia Líquida/métodos , Feminino , Humanos , Medições LuminescentesRESUMO
Tuberculosis control in big cities should be focused on preventing defaulting from treatment, and the prevention of the emergence of multi-drug resistant tuberculosis, and the improvement of treatment success rate. Since it is needed to organize continued case management system starting from hospitalization, discharge, management of regular drug taking at outpatient clinics and final cure, close collaboration should be made between hospital nurses and public health nurses. For this purpose, there should be no difference about the understanding on tuberculosis control between clinical and public health nurses. This research was aimed to examine "How much interest and recognition do hospital nurses have about tuberculosis control." The Aichi Nursing Association cooperated with this research. The results showed that 60% of tuberculosis ward nurses were more than forty years old, and they have served more than ten years in TB word. The levels of understanding on tuberculosis among nurses working in tuberculosis hospitals were much higher than those in general hospitals. However, it is necessary to organize a collaboration system between hospitals and public health center to improve TB case management.
