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1.
Langmuir ; 39(32): 11379-11387, 2023 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-37531145

RESUMO

Block copolymer-mediated self-assembly of colloidal nanoparticles has attracted great attention for fabricating various nanoparticle arrays. We have previously shown that silica nanoparticles (SNPs) assemble into ring-like nanostructures in the presence of temperature-responsive block copolymers poly[(2-ethoxyethyl vinyl ether)-block-(2-methoxyethyl vinyl ether)] (PEOVE-PMOVE) in an aqueous phase. The ring-like nanostructures formed within an aggregate of PEOVE-PMOVE when the temperature was increased to 45 °C, at which the polymer is amphiphilic. Herein, we report that SNPs assemble into ring-like nanostructures even with a different temperature-responsive, amphiphilic block copolymer poly(propylene oxide)-block-poly(ethylene oxide) (PPO-PEO) at 45 °C. Field-emission scanning electron microscopy for SNP assemblies that were spin-coated on a substrate indicated that SNP first assembled into chain-like nanostructures and then bent into closed loops over several days. In contrast, in situ small-angle X-ray diffraction measurements revealed the formation of SNP nanorings within 75 s at 45 °C in the liquid phase. These results indicated that ring-like assembly of SNPs occurs quickly in the liquid phase, but the slow formation of Si-O-Si bonds between SNPs leads to their structure being destroyed by spin-coating. Intriguingly, SNPs with a diameter of 15 nm form a well-defined nanoring structure, with five SNPs located at the vertex points of a regular pentagon. Additionally, small-angle neutron scattering, where the contrast of the solvent (a mixture of H2O and D2O) matches that of SNPs, clarified that SNPs are contained within the spherical micelle formed from PPO-PEO. This work offers a facile and versatile approach to preparing ring-like arrays from inorganic colloidal nanoparticles, leading to applications including sensing, catalysis, and nanoelectronics.

2.
Regen Biomater ; 10: rbac111, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36683748

RESUMO

Strategic materials design is essential for the development of small-diameter, tissue-engineered vascular grafts. Self-assembled nanofibers of elastin-like polypeptides represent promising vascular graft components as they replicate the organized elastin structure of native blood vessels. Further, the bioactivity of nanofibers can be modified by the addition of functional peptide motifs. In the present study, we describe the development of a novel nanofiber-forming elastin-like polypeptide (ELP) with an arginine-glutamic acid-aspartic acid-valine (REDV) sequence. The biological characteristics of the REDV-modified ELP nanofibers relevant to applications in vascular grafting were compared to ELP without ligands for integrin, ELP with arginine-glycine-aspartic acid (RGD) sequence, collagen and cell culture glass. Among them, REDV-modified ELP nanofibers met the preferred biological properties for vascular graft materials, i.e. (i) inhibition of platelet adhesion and activation, (ii) endothelial cell adhesion and proliferation and (iii) maintenance of smooth muscle cells in a contractile phenotype to prevent cell overgrowth. The results indicate that REDV-modified ELP nanofibers represent promising candidates for the further development of small-diameter vascular grafts.

3.
Sci Technol Adv Mater ; 23(1): 341-351, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35693889

RESUMO

Ceramic biomaterials have been used for the treatment of bone defects and have stimulated intense research on such materials. We have previously reported that a salt composed of calcium ions and a phosphate ester (SCPE) transformed into hydroxyapatite (HAp) in a simulated body fluid (SBF) modified with alkaline phosphatase (ALP), and proposed SCPEs as a new category of ceramic biomaterials, namely bioresponsive ceramics. However, the factors that affect the transformation of SCPEs to HAp in the SBF remained unclear. Therefore, in this study, we investigated the behaviour of calcium salts of methyl phosphate (CaMeP), ethyl phosphate (CaEtP), butyl phosphate (CaBuP), and dodecyl phosphate (CaDoP) in SBF with and without ALP modification. For the standard SBF, an X-ray diffraction (XRD) analysis indicated that these SCPEs did not readily transform into calcium phosphate. However, CaMeP, CaEtP, and CaBuP were transformed into HAp and octacalcium phosphate in the SBF modified with ALP; therefore, these SCPEs can be categorised as bioresponsive ceramics. Although CaDoP did not exhibit a sufficient response to ALP to be detected by XRD, it is likely to be a bioresponsive ceramic based on the results of morphological observations. The transformation rate for the SCPEs decreased with increasing size of the linear alkyl group of the phosphate esters. The rate-determining steps for the transformation reaction of the SCPEs were changed from the dissolution of the SCPEs to the hydrolysis of the phosphate esters with increasing size of the phosphate ester alkyl groups. These findings contribute to designing novel bioresponsive ceramic biomaterials.

4.
Sci Technol Adv Mater ; 22(1): 1000-1012, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34924816

RESUMO

The present study aims to develop a layered zirconium phosphate/phosphonate (LZP) powder to control the release of therapeutic inorganic ions. Organically modified LZPs were successfully prepared with various contents of phenyl groups via a reflux method in an aqueous solution containing phosphoric and phenylphosphonic acids. Powder X-ray diffraction analysis and Fourier transform infrared spectroscopy revealed that the crystal structure of the synthesized LZP samples was identical to that of α-zirconium phosphate, even after modification. The amount of incorporated organic molecules increased with increasing molar fractions of phenylphosphonic acid in the starting composition, as determined from the thermal analysis. Cobalt ion (Co2+), a type of therapeutic inorganic ion, was incorporated into the organically modified LZP through treatment with an acetonitrile solution containing tetrabutylammonium ions, followed by treatment with an acetonitrile solution containing CoCl2. The amount of incorporated Co2+ depended on the concentration of the phenyl groups. Furthermore, the highest amount of Co2+ was incorporated in the sample (ZP-Ph-0.5) prepared with equimolar phosphoric/phenylphosphonic acid. The ZP-Ph-0.5 sample additionally showed the ability to incorporate copper or iron ions (Cu2+ or Fe3+). The incorporated ion, either Co2+ or Cu2+, was continuously released from the ZP-Ph-0.5 sample in a saline solution over a period of three weeks, whereas the release of Fe3+ was negligible. The quantity of Co2+ released was higher than that of Cu2+. The controlled release of Co2+ from the ZP-Ph-0.5 sample was also observed in a simulated body fluid that mimicked the ionic concentration of human blood plasma. These results confirm that a specific degree of phenyl modification makes LZP a candidate host material for releasing therapeutic inorganic ions.

5.
Int J Mol Sci ; 22(8)2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33921095

RESUMO

Physically crosslinked hydrogels with thixotropic properties attract considerable attention in the biomedical research field because their self-healing nature is useful in cell encapsulation, as injectable gels, and as bioinks for three-dimensional (3D) bioprinting. Here, we report the formation of thixotropic hydrogels containing nanofibers of double-hydrophobic elastin-like polypeptides (ELPs). The hydrogels are obtained with the double-hydrophobic ELPs at 0.5 wt%, the concentration of which is an order of magnitude lower than those for previously reported ELP hydrogels. Although the kinetics of hydrogel formation is slower for the double-hydrophobic ELP with a cell-binding sequence, the storage moduli G' of mature hydrogels are similar regardless of the presence of a cell-binding sequence. Reversible gel-sol transitions are demonstrated in step-strain rheological measurements. The degree of recovery of the storage modulus G' after the removal of high shear stress is improved by chemical crosslinking of nanofibers when intermolecular crosslinking is successful. This work would provide deeper insight into the structure-property relationships of the self-assembling polypeptides and a better design strategy for hydrogels with desired viscoelastic properties.


Assuntos
Elastina/química , Hidrogéis/química , Interações Hidrofóbicas e Hidrofílicas , Nanofibras/química , Peptídeos/química , Sequência de Aminoácidos , Reagentes de Ligações Cruzadas/química , Módulo de Elasticidade , Iridoides/química , Nanofibras/ultraestrutura , Reologia
6.
Commun Chem ; 4(1): 4, 2021 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36697512

RESUMO

Octacalcium phosphate (OCP; Ca8(HPO4)2(PO4)4 ∙ 5H2O) is a precursor of hydroxyapatite found in human bones and teeth, and is among the inorganic substances critical for hard tissue formation and regeneration in the human body. OCP has a layered structure and can incorporate carboxylate ions into its interlayers. However, studies involving the incorporation of tetracarboxylic and multivalent (pentavalent and above) carboxylic acids into OCP have not yet been reported. In this study, we investigate the incorporation of pyromellitic acid (1,2,4,5-benzenetetracarboxylic acid), a type of tetracarboxylic acid, into OCP. We established that pyromellitate ions could be incorporated into OCP by a wet chemical method using an acetate buffer solution containing pyromellitic acid. The derived OCP showed a brilliant blue emission under UV light owing to the incorporated pyromellitate ions. Incorporation of a carboxylic acid into OCP imparted new functions, which could enable the development of novel functional materials for biomedical applications.

7.
Materials (Basel) ; 13(16)2020 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-32824485

RESUMO

Tearable and fillable implants are used to facilitate surgery. The use of implants that can generate heat and release a drug in response to an exogenous trigger, such as an alternating magnetic field (AMF), can facilitate on-demand combined thermal treatment and chemotherapy via remote operation. In this study, we fabricated tearable sponges composed of collagen, magnetite nanoparticles, and anticancer drugs. Crosslinking of the sponges by heating for 6 h completely suppressed undesirable drug release in saline at 37 °C but allowed drug release at 45 °C. The sponges generated heat immediately after AMF application and raised the cell culture medium temperature from 37 to 45 °C within 15 min. Heat generation was controlled by switching the AMF on and off. Furthermore, in response to heat generation, drug release from the sponges could be induced and moderated. Thus, remote-controlled heat generation and drug release were achieved by switching the AMF on and off. The sponges destroyed tumor cells when AMF was applied for 15 min but not when AMF was absent. The tearing and filling properties of the sponges may be useful for the surgical repair of bone and tissue defects. Moreover, these sponges, along with AMF application, can facilitate combined thermal therapy and chemotherapy.

8.
RSC Adv ; 10(59): 36051-36057, 2020 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-35517070

RESUMO

This study aims to evaluate the in vitro cytocompatibility of layered zirconium phosphate (ZP) and its derivative material that was organically modified using glycerophosphate (ZGP). The ZP and ZGP particles were prepared via a reflux method in an aqueous solution containing phosphoric acid. The field emission scanning electron microscopy showed the prepared samples were fine particles with 70-100 nm diameter. X-ray diffraction and Raman spectrometry indicated the presence of a layered crystal structure. The interlayer distance of ZP was estimated to be 0.76 nm from the 002 diffraction. Modification of ZP with ß-glycerophosphate, lead to expansion of the interlayer distance of 0.85 nm. Grazing incidence X-ray diffraction and Raman spectrometry showed that the crystal structures of ZP and ZGP were maintained even after the samples were coated onto polyethylene (PE) substrates via hot pressing. The water droplet contact angles on the PE substrates coated with the ZP and ZGP particles (ZP/PE and ZGP/PE) were 2 ∼ 6° lesser than that on the uncoated PE substrate. After human adipose-derived stem cells (hASCs) were cultured on the substrates, 2.5-3.5 times higher numbers of adhered cells were observed on the substrates coated with ZP and ZGP than on the uncoated PE substrates and 1.1-1.6 times higher than on the substrate coated with hydroxyapatite particles (HAp/PE). Increasing cell numbers were observed after culturing for 24 h, indicating that the ZP/PE and ZGP/PE showed low cytotoxicity to the hASCs. Furthermore, the ZP/PE showed the highest area of hASC adhesion among all the samples. These results highlight the possibility that layered zirconium phosphate and its organically modified substances can be applied to biomaterials for tissue repair.

9.
Int J Mol Sci ; 20(24)2019 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-31842263

RESUMO

Elastin-like polypeptides (ELPs) are promising candidates for fabricating tissue-engineering scaffolds that mimic the extracellular environment of elastic tissues. We have developed a "double-hydrophobic" block ELP, GPG, inspired by non-uniform distribution of two different hydrophobic domains in natural elastin. GPG has a block sequence of (VGGVG)5-(VPGXG)25-(VGGVG)5 that self-assembles to form nanofibers in water. Functional derivatives of GPG with appended amino acid motifs can also form nanofibers, a display of the block sequence's robust self-assembling properties. However, how the block length affects fiber formation has never been clarified. This study focuses on the synthesis and characterization of a novel ELP, GPPG, in which the central sequence (VPGVG)25 is repeated twice by a short linker sequence. The self-assembly behavior and the resultant nanostructures of GPG and GPPG were when compared through circular dichroism spectroscopy, atomic force microscopy, and transmission electron microscopy. Dynamic rheology measurements revealed that the nanofiber dispersions of both GPG and GPPG at an extremely low concentration (0.034 wt%) exhibited solid-like behavior with storage modulus G' > loss modulus G" over wide range of angular frequencies, which was most probably due to the high aspect ratio of the nanofibers that leads to the flocculation of nanofibers in the dispersion.


Assuntos
Elastina/química , Nanofibras/química , Peptídeos/química , Sequência de Aminoácidos , Interações Hidrofóbicas e Hidrofílicas , Microscopia de Força Atômica , Peso Molecular , Nanofibras/ultraestrutura , Reologia , Análise Espectral
10.
Langmuir ; 34(26): 7751-7758, 2018 07 03.
Artigo em Inglês | MEDLINE | ID: mdl-29878793

RESUMO

Block copolymer-mediated self-assembly of colloidal nanoparticles has attracted great attention for the fabrication of a wide variety of nanoparticle arrays. We have previously shown that silica nanospheres (SNSs) 15 nm in diameter assemble into ring-like nanostructures in the presence of amphiphilic block copolymers poly[(2-ethoxyethyl vinyl ether)- block-(2-methoxyethyl vinyl ether)] (EOVE-MOVE) in an aqueous phase. Here, the effects of particle size of SNSs on this polymer-mediated self-assembly are studied systematically using scanning electron microscopy to observe SNSs of seven different sizes between 13 to 42 nm. SNSs of 13, 16, 19, and 21 nm in diameter assemble into nanorings in the presence of EOVE-MOVE. In contrast, larger SNSs of 26, 34, and 42 nm aggregate heavily, form chain-like networks, and remain dispersed, respectively, instead of forming ring-like nanostructures. The assembly trend for 26-42 nm-SNSs agrees with that expected from the increased colloidal stability for larger particles. Time-course observation for the assembled morphology of 16 nm-SNSs reveals that the nanorings, once formed, assemble further into network-like structures, as if the nanorings behave as building units for higher-order assembly. This indicates that the ring-like assembly is a fast process that can proceed onto random colloidal aggregation. Detailed analysis of nanoring structures revealed that the average number of SNSs comprising one ring decreased from 5.0 to 3.1 with increasing the SNS size from 13 to 21 nm. A change in the number of ring members was also observed when the length of EOVE-MOVE varied while the size of SNSs was fixed. Dynamic light scattering measurements and atomic force microscopy confirmed the SNSs/polymer composite structures. We hypothesize that a stable composite morphology may exist that is influenced by both the size of SNSs and the polymer molecular structures.

11.
Langmuir ; 33(38): 10077-10083, 2017 09 26.
Artigo em Inglês | MEDLINE | ID: mdl-28857564

RESUMO

Surface morphology is a key factor that might significantly influence the properties of biomaterials. In this study, periodic surface-ring structures have been constructed for calcium phosphate thin films via biomineralization-inspired crystallization process. The patterned octacalcium phosphate crystals have been obtained on poly(2-hydroxyethyl methacrylate) (PHEMA) matrix in the presence of poly(acrylic acid) (PAA). The patterned surface morphologies of the crystal thin films could be tuned by the amount of PAA additives. In addition, the rapid and topotactic transformation to hydroxyapatite (HAP) thin films with surface-ring structures has also been achieved. This study may provide new strategy toward the design of functional calcium phosphate-based thin-film hybrids.

12.
J Biomed Mater Res A ; 105(9): 2475-2484, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28486777

RESUMO

We have recently developed a novel double-hydrophobic elastin-like triblock polypeptide called GPG, designed after the uneven distribution of two different hydrophobic domains found in elastin, an extracellular matrix protein providing elasticity and resilience to tissues. Upon temperature trigger, GPG undergoes a sequential self-assembling process to form flexible beaded nanofibers with high homogeneity and excellent dispersibility in water. Given that GPG might be a potential elastin-mimetic material, we sought to explore the biological activities of this block polypeptide. Besides GPG, several functionalized derivatives were also constructed by fusing functional motifs such as KAAK or KAAKGRGDS at the C-terminal of GPG. Although the added motifs affected the kinetics of fiber formation and ß-sheet contents, all three GPGs assembled into beaded nanofibers at the physiological temperature. The resulting GPG nanofibers preserved their beaded structures in cell culture medium; therefore, they were coated on polystyrene substrates to study their cytocompatibility toward mouse embryonic fibroblasts, NIH-3T3. Among the three polypeptides, GPG having the cell-binding motif GRGDS derived from fibronectin showed excellent cell adhesion and cell proliferation properties compared to other conventional materials, suggesting its promising applications as extracellular matrices for mammalian cells. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2475-2484, 2017.


Assuntos
Elastina/química , Elastina/farmacologia , Interações Hidrofóbicas e Hidrofílicas , Peptídeos/química , Peptídeos/farmacologia , Motivos de Aminoácidos , Sequência de Aminoácidos , Animais , Adesão Celular/efeitos dos fármacos , Contagem de Células , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Dicroísmo Circular , Humanos , Proteínas Imobilizadas/farmacologia , Camundongos , Microscopia de Força Atômica , Células NIH 3T3 , Nanofibras/química , Temperatura , Água/química
13.
J Nanosci Nanotechnol ; 16(3): 3077-83, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27455764

RESUMO

α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2) receives great attention for bone repairing due to its biodegradability and capability of transformation to human bone's main inorganic components, hydroxyapatite (HAp). α-TCP porous scaffold is easily procurable by sintering of the low-temperature polymorph of TCP, ß-TCR Still, porous body of α-TCP is too brittle to being handled and shaped, limiting its clinical application as implant materials. To improve mechanical properties of α-TCP porous scaffold, the present study focused on coating of a type of polysaccharides on α-TCP scaffolds. Gellan gum was chosen as the polysaccharide for coating because of its biodegradability as well as the potential acting as substrate for HAp deposition during hydration of α-TCP after exposure to body fluid. After coating of gellan gum on α-TCP scaffolds with porosity of 75 vol%, the compressive strength increased from 0.45 MPa to around 2.00 MPa. Among the coated scaffold, the maximum compressive strength, 3.97 MPa, was obtained on the scaffold with porosity of 63 vol%. Improvement of mechanical properties of α-TCP/gellan gum composites was achieved to show easy handling performance for a bone substitute for tissue repairing. The dissolving rate of the coated scaffolds was also controlled by adjusting the concentration of GG solutions.


Assuntos
Osso e Ossos , Fosfatos de Cálcio/química , Polissacarídeos Bacterianos/química , Engenharia Tecidual , Alicerces Teciduais , Microscopia Eletrônica de Varredura , Porosidade , Difração de Raios X
14.
Front Oral Biol ; 17: 33-8, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26201274

RESUMO

Bioactive ceramics are known to exhibit specific biological affinities and are able to show direct integration with surrounding bone when implanted in bony defects. However, their inadequate mechanical properties, such as low fracture toughness and high Young's modulus in comparison to natural bone, limit their clinical application. Bone is a kind of organic-inorganic composite where apatite nanocrystals are precipitated onto collagen fibre networks. Thus, one way to address these problems is to mimic the natural composition of bone by using bioactive ceramics via material designs based on organic-inorganic composites. In this chapter, the current research on the development of the various organic-inorganic composites designed for biomaterial applications has been reviewed. Various compounds such as calcium phosphate, calcium sulphate and calcium carbonate can be used for the inorganic phases to design composites with the desired mechanical and biological properties of bone. Not only classical mechanical mixing but also coating of the inorganic phase in aqueous conditions is available for the fabrication of such composites. Organic modifications using various polymers enable the control of the crystalline structure of the calcium carbonate in the composites. These approaches on the fabrication of organic-inorganic composites provide important options for biomedical materials with novel functions.


Assuntos
Materiais Biocompatíveis/química , Substitutos Ósseos/química , Cerâmica/química , Materiais Biomiméticos/química , Carbonato de Cálcio/química , Fosfatos de Cálcio/química , Sulfato de Cálcio/química , Humanos
15.
Dalton Trans ; 44(17): 7943-50, 2015 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-25826693

RESUMO

Octacalcium phosphate (OCP) is composed of apatitic and hydrated layers, and can incorporate dicarboxylate ions in its hydrated layers by substitution of HPO4(2-). The (100) interplanar spacing of OCP is increased by incorporation of dicarboxylate ions. Herein, we report continuous expansion of the interplanar spacing of OCP by incorporation of dicarboxylate ions with a side chain. We synthesized OCP with incorporated succinic acid (Suc) and mercaptosuccinic acid (Msuc) by a wet chemical process. The (100) interplanar spacing of OCP synthesized with Suc increased non-continuously as the amount of Suc used in the synthesis increased. In contrast, the (100) interplanar spacing of OCP synthesized with Msuc increased continuously with the amount of Msuc used during synthesis, and thus in the OCP. The values of the (100) interplanar spacing of OCP synthesized with Msuc first increased and then became constant as the amount of Msuc increased. OCP with incorporated Msuc formed a continuous solid solution. The expansion of the (100) interplanar spacing of OCP with incorporated Suc and Msuc depended on the chemical structure of the incorporated dicarboxylate ions. The only structural difference between Suc and Msuc is a mercapto (R-SH) side chain. Therefore, continuous solid solution formation was most likely induced by the R-SH side chain of Msuc. These results suggest that incorporation of dicarboxylic acids with a side chain is one approach to obtain OCP with arbitrary interlayer distance.

16.
J Mater Sci Mater Med ; 26(2): 115, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25665841

RESUMO

The aim of this study was to propose and validate a new unified method for testing dissolution rates of bioactive glasses and their variants, and the formation of calcium phosphate layer formation on their surface, which is an indicator of bioactivity. At present, comparison in the literature is difficult as many groups use different testing protocols. An ISO standard covers the use of simulated body fluid on standard shape materials but it does not take into account that bioactive glasses can have very different specific surface areas, as for glass powders. Validation of the proposed modified test was through round robin testing and comparison to the ISO standard where appropriate. The proposed test uses fixed mass per solution volume ratio and agitated solution. The round robin study showed differences in hydroxyapatite nucleation on glasses of different composition and between glasses of the same composition but different particle size. The results were reproducible between research facilities. Researchers should use this method when testing new glasses, or their variants, to enable comparison between the literature in the future.


Assuntos
Apatitas/química , Materiais Biomiméticos/química , Materiais Biomiméticos/normas , Líquidos Corporais/química , Cerâmica/química , Vidro/química , Teste de Materiais/normas , Apatitas/normas , Cerâmica/análise , Cerâmica/normas , Vidro/análise , Vidro/normas , Internacionalidade , Teste de Materiais/métodos , Tamanho da Partícula , Padrões de Referência , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
17.
Mater Sci Eng C Mater Biol Appl ; 48: 279-86, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25579924

RESUMO

Hydroxyapatite formation on the surfaces of implanted materials plays an important role in osteoconduction of bone substitutes in bone tissues. Titania hydrogels are known to instigate hydroxyapatite formation in a solution mimicking human blood plasma. To date, the relationship between the surface characteristics of titania and hydroxyapatite formation on its surface remains unclear. In this study, titania powders with varying surface characteristics were prepared by addition of manganese or iron to examine hydroxyapatite formation in a type of simulated body fluid (Kokubo solution). Hydroxyapatite formation was monitored by observation of deposited particles with scale-like morphology on the prepared titania powders. The effect of the titania surface characteristics, i.e., crystal structure, zeta potential, hydroxy group content, and specific surface area, on hydroxyapatite formation was examined. Hydroxyapatite formation was observed on the surface of titania powders that were primarily anatase, and featured a negative zeta potential and low specific surface areas irrespective of the hydroxy group content. High specific surface areas inhibited the formation of hydroxyapatite because calcium and phosphate ions were mostly consumed by adsorption on the titania surface. Thus, these surface characteristics of titania determine its osteoconductivity following exposure to body fluid.


Assuntos
Durapatita/química , Ferro/química , Manganês/química , Titânio/química , Materiais Biocompatíveis/química , Líquidos Corporais/química , Microscopia de Força Atômica , Pós , Soluções , Espectrometria por Raios X , Propriedades de Superfície
18.
Biomater Sci ; 2(9): 1172-1185, 2014 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-25485105

RESUMO

We demonstrate utilization of star-shaped polymers as high-density polymer brush coatings and their effectiveness to inhibit the adhesion of platelets and bacteria. Star polymers consisting of poly(2-hydroxyethyl methacrylate) (PHEMA) and/or poly(methyl methacrylate) (PMMA), were synthesized using living radical polymerization with a ruthenium catalyst. The polymer coatings were prepared by simple drop casting of the polymer solution onto poly(ethylene terephthalate) (PET) surfaces and then dried. Among the star polymers prepared in this study, the PHEMA star polymer (star-PHEMA) and the PHEMA/PMMA (mol. ratio of 71/29) heteroarm star polymer (star-H71M29) coatings showed the highest percentage of inhibition against platelet adhesion (78-88% relative to noncoated PET surface) and Escherichia coli (94-97%). These coatings also showed anti-adhesion activity against platelets after incubation in Dulbecco's phosphate buffered saline or surfactant solution for 7 days. In addition, the PMMA component of the star polymers increased the scratch resistance of the coating. These results indicate that the star-polymer architecture provides high polymer chain density on PET surfaces to prevent adhesion of platelets and bacteria, as well as coating stability and physical durability to prevent exposure of bare PET surfaces. The star polymers provide a simple and effective approach to preparing anti-adhesion polymer coatings on biomedical materials against the adhesion of platelets and bacteria.

19.
Biol Pharm Bull ; 36(11): 1670-5, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24189410

RESUMO

Several varieties of ceramics, such as Bioglass-type glasses, sintered hydroxyapatite and glass-ceramic A-W, exhibit specific biological affinity, i.e., direct bonding to surrounding bone, when implanted in bony defects. These bone-bonding ceramics are called bioactive ceramics and are utilized as important bone substitutes in the medical field. However, there is a limitation to their clinical applications because of their inappropriate mechanical properties. Natural bone takes a kind of organic-inorganic composite, where apatite nanocrystals are precipitated on collagen fibers. Therefore, problems with the bioactive ceramics can be solved by material design based on the composites. In this paper, current research topics on the development of bioactive organic-inorganic composites inspired by actual bone microstructure have been reviewed in correlation with preparation methods and various properties. Several kinds of inorganic components have been found to exhibit bioactivity in the body environment. Combination of the inorganic components with various organic polymers enables the development of bioactive organic-inorganic composites. In addition, novel biomedical applications of the composites to drug delivery systems, scaffolds for tissue regeneration and injectable biomaterials are available by combining drugs or biological molecules with appropriate control of its microstructure.


Assuntos
Substitutos Ósseos/química , Cerâmica/química , Nanocompostos/química , Humanos , Polímeros/química
20.
J Mater Sci Mater Med ; 24(12): 2677-82, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23918526

RESUMO

Glass ionomer cements (GICs) are composed of an acid degradable glass, polyacrylic acid and water. Sol-gel processing to prepare the glass phase has certain advantages, such as the ability to employ lower synthesis temperatures than melt quenching and glasses that are reported to have higher purity. A previous study reported the effects of glass synthesis route on GIC fabrication. However, in that study, the sol-gel derived glass exhibited a reduced concentration of cations. This study investigates increasing the cation content of a sol-gel derived glass, 12CaO.4SrO.36ZnO.48SiO2 (molar ratio) by heating before aging to reduce dissolution of cations. This glass was prepared by both sol-gel and melt-quenched routes. GICs were subsequently prepared using both glasses. The resultant cement based on the sol-gel derived glass had a shorter working time than the cement based on the melt-quenched one. Contrary to this, setting time was considerably longer for the cement based on the sol-gel derived glass than for the cement based on the melt-quenched one. The cements based on the sol-gel derived glass were stronger in both compression and biaxial flexure than the cements prepared from the melt-quenched glass. The differences in setting and mechanical properties were associated with both cation content in the glass phase and the different surface area of the resultant cements.


Assuntos
Compostos de Cálcio/química , Cimentos de Ionômeros de Vidro/química , Vidro/química , Óxidos/química , Silício/química , Estrôncio/química , Óxido de Zinco/química , Cátions , Força Compressiva , Cimentos Dentários/química , Tamanho da Partícula , Transição de Fase , Pós , Pressão , Estresse Mecânico , Temperatura
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