1.
Nucleic Acids Res Suppl
; (1): 125-6, 2001.
Artigo
em Inglês
| MEDLINE
| ID: mdl-12836296
RESUMO
Catalysis of Group I intron ribozymes is carried out by its core region consisting of two helical domains P4-P6 and P3-P7. Recently, our laboratory showed that a mutant Group I ribozyme lacking both the P4-P6 domain and the base-triples can perform the trans-esterification reactions. The result demonstrates that the elements required for splicing are concentrated in the P3-P7 domain. Based on this result, we carried out in vitro selection experiment starting from newly constructed libraries in the ribozyme lacking the P4-P6 domain and the base-triples. This selection experiment showed unexpected divergency on the limited sequence space for the active ribozymes.