Omics approaches to understand the MADS-box gene family in common bean (Phaseolus vulgaris L.) against drought stress.

MADS-box genes are known to play important roles in diverse aspects of growth/devolopment and stress response in several plant species. However, no study has yet examined about MADS-box genes in P. vulgaris. In this study, a total of 79 PvMADS genes were identified and classified as type I and type II according to the phylogenetic analysis. While both type I and type II PvMADS classes were found to contain the MADS domain, the K domain was found to be present only in type II PvMADS proteins, in agreement with the literature. All chromosomes of the common bean were discovered to contain PvMADS genes and 17 paralogous gene pairs were identified. Only two of them were tandemly duplicated gene pairs (PvMADS-19/PvMADS-23 and PvMADS-20/PvMADS-24), and the remaining 15 paralogous gene pairs were segmentally duplicated genes. These duplications were found to play an important role in the expansion of type II PvMADS genes. Moreover, the RNAseq and RT-qPCR analyses showed the importance of PvMADS genes in response to drought stress in P. vulgaris.

The genome-wide characterization of WOX gene family in Phaseolus vulgaris L. during salt stress.

The WUSCHEL-Related Homeobox (WOX) family is a type of homeobox transcription factor superfamily and its members perform many functions ranging from plant embryonic growth to organ formation in plants. Although the WOX proteins have been identified and characterized in many plant species, genome-wide identification and characterization of WOX proteins in the Phaseolus vulgaris genome has been performed for the first time in this study. Accordingly, 18 WOX proteins were identified using bioinformatics tools and biochemical/physicochemical properties of these proteins were investigated. Phvul-WOX genes were found to be categorized into three major phylogenetic groups according to the phylogenetic analysis and a total of five segmental duplication events were detected after duplication analysis. Moreover, the Phvul-WOX genes were found to be expressed in different plant tissues at different levels and some stress-related miRNAs have been found to target the Phvul-WOX genes based on miRNA analysis. Additionaly, MDA content, total protein level and catalase enzyme activity analyses were conducted in two P. vulgaris cultivars namely Yakutiye cv. and Zulbiye cv. subjected to 150 mM salt stress. Next, these cultivars were used for screening the expression levels of Phvul-WOX-1, Phvul-WOX-9, Phvul-WOX-11, Phvul-WOX-15 and Phvul-WOX-16 genes in response to salt stress. The insights gained from this study may be of assistance to the researchers who work in this area. Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-022-01208-1.

Identification and Characterization of SRS Genes in Phaseolus vulgaris Genome and Their Responses Under Salt Stress.

SHI-Related Sequence (SRS) transcription factors comprise a protein family with important roles in growth and development. However, the genome-wide study of the SRS protein family has not yet been carried out in the common bean. For this reason, the SRS family has been characterized in depth at both gene and protein levels and several bioinformatics methods have been used. As a result, 10 SRS genes have been identified and their proteins have been phylogenetically categorized into three major groups within the common bean. By investigating duplications that play a major role in the development of gene families, 19 duplication events have been identified in the SRS family (18 segmental and 1 tandem). In addition, using available RNAseq data, comparative expression analysis of Pvul-SRS genes was performed and expression changes in Pvul-SRS-1, 2, 4, 6, 7, and 10 genes were observed under both salt and drought stress. Five Pvul-SRS genes were selected based on RNAseq data (Pvul-SRS-1, 2, 4, 6, and 10) and screened with RT-qPCR in two common bean cultivars (Yakutiye 'salt-resistant' and Zulbiye 'salt-susceptible' cv.). These genes also showed different levels of expression between two common bean cultivars under salt stress conditions and this may explain the responses of Pvul-SRS genes against abiotic stress. In summary, this work is the first study in which in silico identification and characterization of Pvul-SRS genes have been examined at gene expression level. The results could therefore provide the basis for future studies of functional characterization of Pvul-SRS genes.