Room temperature detection of aspergillus flavus volatile organic compounds (VOCs) under simulated conditions using graphene oxide and tin oxide Nanorods (SnO2 NRs-GO).

This study investigated the application of a hybrid nanocomposite of tin oxide nanorods (SnO2 NRs) and graphene oxide (GO) for the chemoresistive detection of some volatile compounds (hexanal, benzaldehyde, octanal, 1-octanol, and ethyl acetate vapours) emitted by Aspergillus flavus under simulated conditions. The synthesised materials were characterised using various analytical techniques, including high-resolution transmission electron microscopy (HR-TEM), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) analysis, and Fourier transform infrared spectroscopy (FTIR). Three sensors were fabricated: individual nanomaterials (i.e., SnO2 and GO) and composites (SnO2-GO). The results showed that SnO2 NRs had limited sensitivity as a sensor, while GO-based sensors responded to various analyte vapours. However, the incorporation of SnO2 NRs into GO layers resulted in synergistic effects and improved sensor performance. The sensors' sensitivity, selectivity, recovery, and response times were quantitatively determined from the sensors' response curves. The nanocomposite sensor demonstrated superior sensitivity and selectivity for analyte vapours with acceptable response and recovery times. In addition, the sensor was insensitive to humidity and showed robust performance up to 62% RH, although sensor drift occurred at 70% RH. This study highlights the promising potential of using SnO2 NRs-GO composite-based sensor for sensitive and selective detection of analyte vapours, which has significant implications for food safety and environmental monitoring applications.

Morphed aflaxotin concentration produced by Aspergillus flavus strain VKMN22 on maize grains inoculated on agar culture.

This study identified and monitored the levels of aflatoxins (B1 and B2) produced by Aspergillus flavus isolate VKMN22 (OP355447) in maize samples sourced from a local shop in Johannesburg, South Africa. Maize samples underwent controlled incubation after initial rinsing, and isolates were identified through morphological and molecular methods. In another experiment, autoclaved maize grains were intentionally re-inoculated with the identified fungal isolate using spore suspension (106 spore/mL), after which 1 g of the contaminated maize sample was inoculated on PDA media and cultured for seven days. The aflatoxin concentrations in the A. flavus contaminated maize inoculated on culture media was monitored over seven weeks and then measured using liquid chromatography-mass spectroscopy (LC-MS). Results confirmed the successful isolation of A. flavus strain VKMN22 with accession number OP355447, which consistently produced higher levels of AFB1 compared to AFB2. AF concentrations increased from week one to five, then declined in week six and seven. AFB1 levels ranged from 594.3 to 9295.33 µg/kg (week 1-5) and then reduced from 5719.67 to 2005 µg/kg in week six and seven), while AFB2 levels ranged from 4.92 to 901.67 µg/kg (weeks 1-5) and then degraded to 184 µg/kg in week six then 55.33 µg/kg (weeks 6-7). Levene's tests confirmed significantly higher mean concentrations of AFB1 compared to AFB2 (p ≤ 0.005). The study emphasizes the importance of consistent biomonitoring for a dynamic understanding of AF contamination, informing accurate prevention and control strategies in agricultural commodities thereby safeguarding food safety.

Concomitant in Situ FTIR and Impedance Measurements To Address the 2-Methylcyclopentanone Vapor-Sensing Mechanism in MnO2-Polymer Nanocomposites.

Polymer nanocomposite-based sensors were prepared using cellulose acetate (CA), carbon nanoparticles (CNPs), and manganese dioxide (MnO2) nanorods to detect and to understand the sensing mechanism of 2-methylcyclopentanone vapor. A sensor with a mass ratio of 1:1.5:3 of MnO2/CNPs/CA as well as MnO2/CA and MnO2/CNP composite and MnO2 sensors were prepared. The sensor with the three sensing materials combined exhibited an enhancement of response for 2-methylcyclopentanone vapor, ascribed to a synergistic effect between MnO2/CNPs/CA. An in situ Fourier-transform infrared (FTIR)-combined online LCR meter setup was used to understand the sensing mechanism of the sensor. The sensing mechanism involved a deep oxidation decomposition of the analyte to CO2. This was confirmed from the in situ FTIR-combined online LCR meter results, where a new distinct CO2 bending mode IR band was recorded. To optimize the performance of the sensor, the composites were prepared by varying the amount of metal oxide added into the composites; sensor A (composition of mass ratio 1:1.5:3), sensor B (composition of mass ratio 2:1.5:3), and sensor C (composition of mass ratio 2.5:1.5:3); their compositions are MnO2/CNPs/CA. The performance of sensor B was higher than that of the other two sensors. The sensors also show relatively good response-recovery time. All fabricated sensors were found to have the sensing ability regenerated after the analyte was removed from the system without losing its sensing and recovery abilities. The structural and morphological features of the samples were characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and Raman spectroscopy.