Assuntos
Brucella abortus/isolamento & purificação , Brucelose/veterinária , Bursite/veterinária , Carpo Animal/microbiologia , Doenças dos Cavalos/diagnóstico , Animais , Anticorpos Antibacterianos/sangue , Brucella abortus/imunologia , Brucelose/diagnóstico , Brucelose/microbiologia , Brucelose/transmissão , Bursite/diagnóstico , Bursite/microbiologia , Carpo Animal/patologia , Doenças dos Cavalos/microbiologia , Doenças dos Cavalos/transmissão , Cavalos , Masculino , NigériaAssuntos
Surtos de Doenças/veterinária , Mannheimia haemolytica/isolamento & purificação , Pasteurelose Pneumônica/epidemiologia , Pasteurelose Pneumônica/microbiologia , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/microbiologia , Animais , Mannheimia haemolytica/classificação , Nigéria/epidemiologia , Sorotipagem , OvinosRESUMO
This is the first report on the isolation of Klebsiella planticola from neonates. Over a period of 1 year, oropharyngeal and rectal swab specimens from 131 newborns on a neonatal ward were monitored for Klebsiella spp. Thirteen strains of K. planticola could be isolated, and these represented 9% of all Klebsiella spp. found. Capsule type K27 predominated, followed by capsule type K68. Our findings suggest a significant rate of colonization by K. planticola among neonates.
Assuntos
Unidades de Terapia Intensiva Neonatal , Infecções por Klebsiella/microbiologia , Klebsiella/classificação , Klebsiella/isolamento & purificação , Antibacterianos/farmacologia , Técnicas de Tipagem Bacteriana , Portador Sadio/microbiologia , Humanos , Lactente , Recém-Nascido , Klebsiella/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Orofaringe/microbiologia , Reto/microbiologiaRESUMO
The detection of Legionella sp. in various types of environmental water samples was evaluated using a revised version of the EnviroAmp Legionella kit (Perkin-Elmer), which is based on the polymerase chain reaction (PCR). The new kit employs a modified protocol for the pretreatment of water samples, which is designed to further reduce the inhibitory action of some samples to the PCR. The results of the new kit were compared with those of culture. Seventy-four water samples were examined. Of these samples, 51 were taken from hospital water systems, 16 were household water samples, 5 were from surface water, and 2 were rain water. These samples were examined both by PCR and by culture on MWY Legionella selective agar. Of the 74 samples, 46 (62%) were positive by both test systems. Twenty-five samples (34%) were positive by PCR, but negative by culture. While 3 samples (4%) were negative by both test methods, no sample was negative by PCR and positive by culture. Statistical analysis revealed high positive predictive values of PCR, when L. pneumophila was detected or when the genus Legionella was detected at high intensity. Negative predictive values were high, when the PCR was negative or only weakly positive for the genus Legionella.
