Counselling and selection of homosexual couples in fertility treatment.

Artificial insemination with anonymous donor sperm (AID) is a widespread practice for heterosexual couples in which the male partner appears to be infertile. The Centre for Reproductive Medicine of the Brussels University teaching hospital has been one of the first to extend this form of therapy to lesbian couples. In the course of the past 7 years, 27 lesbian couples have come to the centre with a request for AID. The question of whether children can be brought up within such a frame of reference cannot be dissociated from the question of attitudes towards homosexuality as such. Such attitudes still solicit a whole range of controversial reactions. This paper will deal with the most important findings from investigations thus far carried out on the issue of homosexual parenthood. It also sets out the selection criteria used in the evaluation of applicants. A profile of the homosexual couples selected will be presented, with particular attention to their social status, their psychosexual development and their motivation towards parenthood.

Effect of high pressure, detergents and phospholipase on the break in the Arrhenius plot of Azotobacter nitrogenase.

It is shown that lipids are responsible for the breaks in the Arrhenius plots of Azotobacter nitrogenase. The physical evidence is that temperature at which the break occurs increases with increasing pressure by 20 K/1000 atm. This is in agreement with the pressure dependence of the transition temperature of several synthetic phospholipids. We also find the same pressure dependence for the broad transitions observed in Escherichia coli phosphatidylethanolamine and in the membrane lipids from Azotobacter itself. Detergents and phospholipase remove the break. Reconstruction can be performed only with specific phospholipids.