RESUMO
In experiments on two model systems L929/VSV and RF/ HSV-1 in vitro we have studied the antiviral activity of new structural analogues of tilorone--4,4'-bis[2-(diethyl-amino)ethoxy]diphenyl dihydrochloride and 2-methoxy-carbonyl-4-4'-bis[2-(diethylamino)ethoxy]diphenyl dihydrochloride. In experiments the tested substances were administered in preventive and therapeutic schemes. The preventive scheme confirms the existence of a correlation between IFN production under the influence of tested substances and their antiviral activity. It is shown that diphenyls are able to inhibit the development of viral cytopathic effect induced by DNA- and RNA-containing viruses. Diphenyl derivatives are less toxic than tilorone and could be considered as promising substances for further research to develop new antiviral drugs.
Assuntos
Antivirais/farmacologia , Compostos de Bifenilo/farmacologia , Fibroblastos/efeitos dos fármacos , Herpesvirus Humano 1/efeitos dos fármacos , Vesiculovirus/efeitos dos fármacos , Animais , Linhagem Celular , Efeito Citopatogênico Viral/efeitos dos fármacos , Fibroblastos/virologia , Herpes Simples/tratamento farmacológico , Herpes Simples/virologia , Herpesvirus Humano 1/fisiologia , Humanos , Concentração Inibidora 50 , Dose Máxima Tolerável , Camundongos , Modelos Biológicos , Ratos , Tilorona/farmacologia , Estomatite Vesicular/tratamento farmacológico , Estomatite Vesicular/virologia , Vesiculovirus/fisiologiaRESUMO
Several ovarian disorders in women are associated with autoimmune factors. In this study it was investigated a level of cytokines--TNF-alpha and IFNs in ovarian homogenate and blood serum at ovarian autoimmune damage of mammals. Experimental immune ovarian failure was induced in CBA mice by either immunization with allogenic ovarian extracts or administration of xenogenic anti-ovarian antibodies. Both models were accompanied with abnormalities in production of immunoregulatory cytokines. These data confirm the importance of definition of proinflammatory cytokine level for disclosing immune mechanisms which lay in a pathogenesis autoimmune ovarian diseases of female reproductive organs.
Assuntos
Doenças Autoimunes , Citocinas/sangue , Doenças Ovarianas/imunologia , Animais , Doenças Autoimunes/sangue , Modelos Animais de Doenças , Feminino , Interferons/sangue , Camundongos , Camundongos Endogâmicos CBA , Doenças Ovarianas/sangue , Ovário/imunologia , Fator de Necrose Tumoral alfa/sangueRESUMO
Human cord blood cells were shown to produce interferon-alpha- and -gamma in vitro, both spontaneously and following adequate induction. Transplantation of human blood cord cells to mice led to endogenous interferonogenesis activation, serum interferon levels as well as interferon-alpha and interferon-gamma synthesis by activated peripheral blood leucocytes becoming higher. The maximal interferon accumulation in murine blood sera following transplantation did not coincide with interferonogenesis dynamics observed due to the effect of standard early and late interferon inducers - poly I:C and ridostin, respectively. Interferonogenesis activation was accompanied by increased functional activty of phagocytic system cells.
