Is digital psychiatry really for all? A cross-sectional analysis from two randomized clinical trials.

INTRODUCTION: Digital psychiatry holds promise for expanding accessibility to mental health treatment, but concerns exist regarding its inclusivity and the potential for exacerbation of digital exclusion among vulnerable populations. This study aims to evaluate the inclusivity of digital psychiatry research and interventions, and to explore their potential to worsen digital exclusion. METHODS: We conducted a cross-sectional analysis of sociodemographic data from two clinical trials that utilize psychiatric online treatment modalities in Brazil. Participants were recruited nationwide through digital media platforms. RESULTS: The sample comprised 224 individuals, predominantly female (95.1%) and Caucasian (71.87%) participants, with an average of 15.12 years of schooling. It was observed that White individuals were overrepresented compared to national averages (42.8%). Additionally, participants had a higher average number of years of schooling compared to the national average (10.1 years). Our analysis revealed a clear profile among psychiatric patients with access to and interest in digital interventions, predominantly younger, White, educated, and female individuals. DISCUSSION: As digital therapeutic solutions advance, ensuring their inclusivity and accessibility for vulnerable individuals is crucial. Initiatives to promote digital inclusion and reassess participant recruitment strategies are needed to effectively address digital exclusion. By adopting multifaceted approaches, digital mental health care can be made more effective and accessible to all.

Cytocompatibility and osteogenic differentiation of stem cells from human exfoliated deciduous teeth with cotton cellulose nanofibers for tissue engineering and regenerative medicine.

Cellulose nanofibers (CNFs) are natural polymers with physical-chemical properties that make them very attractive for modulating stem cell differentiation, a crucial step in tissue engineering and regenerative medicine. Although cellulose is cytocompatible, when materials are in nanoscale, they become more reactive, needing to evaluate its potential toxic effect to ensure safe application. This study aimed to investigate the cytocompatibility of cotton CNF and its differentiation capacity induction on stem cells from human exfoliated deciduous teeth. First, the cotton CNF was characterized. Then, the cytocompatibility and the osteogenic differentiation induced by cotton CNF were examined. The results revealed that cotton CNFs have about 6-18 nm diameters, and the zeta potential was -10 mV. Despite gene expression alteration, the cotton CNF shows good cytocompatibility. The cotton CNF induced an increase in phosphatase alkaline activity and extracellular matrix mineralization. The results indicate that cotton CNF has good cytocompatibility and can promote cell differentiation without using chemical inducers, showing great potential as a new differentiation inductor for tissue engineering and regenerative medicine applications.

Small peptides inhibit gut trypsin-like proteases and impair Anticarsia gemmatalis (Lepidoptera: Noctuidae) survival and development.

BACKGROUND: Anticarsia gemmatalis larvae are key defoliating pests of soybean plants. Inorganic insecticides, harmful to the environment and human health, are the main molecules used in the control of this pest. To apply more sustainable management methods, organic molecules with high specificities, such as proteinaceous protease inhibitors, have been sought. Thus, molecular docking studies, kinetics assays, and biological tests were performed to evaluate the inhibitory activity of two peptides (GORE1 and GORE2) rationally designed to inhibit trypsin-like enzymes, which are the main proteases of A. gemmatalis midgut. RESULTS: The molecular docking simulations revealed critical hydrogen bonding patterns of the peptides with key active site residues of trypsin-like proteases of A. gemmatalis and other Lepidopteran insects. The negative values of binding energy indicate that hydrogen bonds potentiate the tight binding of the peptides with trypsin-like proteases, predicting an effective inhibition. The inhibition's rate constants (Ki) were 0.49 and 0.10 mM for GORE1 and GORE2, resulting in effective inhibition of the activity trypsin on the L-BApNA substrate in the in vitro tests, indicating that the peptide GORE2 has higher inhibitory capacity on the A. gemmatalis trypsins. In addition, the two peptides were determined to be reversible competitive inhibitors. The in vivo test demonstrated that the peptides harm the survival and development of A. gemmatalis larvae. CONCLUSION: These results suggest that these peptides are potential candidates in the management of A. gemmatalis larvae and provide baseline information for the design of new trypsin-like inhibitors based on peptidomimetic tools. © 2020 Society of Chemical Industry.

A WW Domain-Containing Protein Forms Immune Nuclear Bodies against Begomoviruses.

The bipartite begomoviruses (Geminiviridae family), which are DNA viruses that replicate in the nucleus of infected cells, encode the nuclear shuttle protein (NSP) to facilitate the translocation of viral DNA from the nucleus to the cytoplasm via nuclear pores. This intracellular trafficking of NSP-DNA complexes is accessorized by the NSP-interacting guanosine triphosphatase (NIG) at the cytosolic side. Here, we report the nuclear redistribution of NIG by AtWWP1, a WW domain-containing protein that forms immune nuclear bodies (NBs) against begomoviruses. We demonstrated that AtWWP1 relocates NIG from the cytoplasm to the nucleus where it is confined to AtWWP1-NBs, suggesting that the NIG-AtWWP1 interaction may interfere with the NIG pro-viral function associated with its cytosolic localization. Consistent with this assumption, loss of AtWWP1 function cuased plants more susceptible to begomovirus infection, whereas overexpression of AtWWP1 enhanced plant resistance to begomovirus. Furthermore, we found that a mutant version of AtWWP1 defective for NB formation was no longer capable of interacting with and relocating NIG to the nucleus and lost its immune function against begomovirus. The antiviral function of AtWWP1-NBs, however, could be antagonized by viral infection that induced either the disruption or a decrease in the number of AtWWP1-NBs. Collectively, these results led us to propose that AtWWP1 organizes nuclear structures into nuclear foci, which provide intrinsic immunity against begomovirus infection.

Associação de dispositivos para acesso a via aérea difícil

Há situações em que o controle da via aérea representa um desafio ao anestesiologista. Tal ocorrência é especialmente real nos casos de paciências com preditores de via aérea difícil, tumorações cervicais friáveis que determinam distorções anatômicas importantes, principalmente em ocasiões de urgência e emergência. Nestes casos a combinação de dispositivos facilitadores de intubação traqueal pode ser de grande valia. A associação do videolaringoscópioaocom broncofibroscópio flexível é um nova combinação plausível, descritas em estudos recente, para controle de via aérea difícil previamente estabelecidas, em adultos. A abordagem multimodal diminuiu ao máximo a ocorrências de complicações e incrementou segurança, favorecendo o controle da via aérea.

Horta orgânica sustentável em comunidade carente de Santana de Parnaíba / Sustainable organic garden in poor community in Santana de Parnaíba

A horta orgânica é uma produção baseada em tecnologias limpas e sustentáveis utilizando recursos sem nenhum agrotóxico. A cada dia as pessoas estão mais preocupadas com o seu bem estar físico, procuram se alimentar de produtos cada vez mais naturais. A horta foi montada nas instalações da instituição Rainha da Paz, sediada em Santana de Parnaíba, SP, foram usadas sementes e plantas orgânicas fornecidas por produtores que foram plantadas na terra preparada com material de compostagem. O objetivo foi a criação de uma horta orgânica e compostagem, possibilitando o aproveitamento de pequenos espaços e a reutilização de materiais que seriam descartados na natureza, capacitando os cuidadores, os adolescentes e os funcionários da instituição cientes das necessidades e desafios para construção de um planeta mais saudável, por meio de capacitação gratuita para a comunidade carente. A primeira colheita dos alimentos produzidos na horta ocorreu em junho quando começamos a utiliza-las no preparo dos alimentos que foram oferecidos as crianças, foram realizadas oficinas culinárias com as mães que passaram a incluir na dieta das crianças, além de iniciar o plantio doméstico. A horta orgânica e a oficina possibilitaram ampliar os conhecimentos e atuação das mães em relação ao plantio e a utilização das plantas com finalidades nutricionais na manutenção do bem-estar destas crianças.(AU)

The organic garden is based on clean and sustainable technologies without the use of any pesticides. Every day people are more and more concerned about their physical well-being, therefore they also try to feed themselves with healthier and natural products. The garden was set up in the premises of Rainha da Paz. We used organic seeds and plants supplied by producers and these were planted in prepared soil with compost material. The aim of this project was to create an organic garden and composting, allowing the use of small spaces and reuse of materials that would be discarded in nature. At the same time we will enable the teenagers and all the staff of the institution to be aware of the necessity and challenges in building a healthier planet, through free training of the disadvantaged community. The first harvest occurred in June, when we started to use the vegetables in the food preparation given to children. At the same time we offered cooking workshop to mothers, who started to include them in their diet and also initiated domestic planting. The organic garden and the workshop enabled mothers to broaden their knowledge and actions in relation to tillage and the use of plants for nutritional purposes to maintain the children's well-being.(AU)

Horta orgânica sustentável em comunidade carente de Santana de Parnaíba / Sustainable organic garden in poor community in Santana de Parnaíba

A horta orgânica é uma produção baseada em tecnologias limpas e sustentáveis utilizando recursos sem nenhum agrotóxico. A cada dia as pessoas estão mais preocupadas com o seu bem estar físico, procuram se alimentar de produtos cada vez mais naturais. A horta foi montada nas instalações da instituição Rainha da Paz, sediada em Santana de Parnaíba, SP, foram usadas sementes e plantas orgânicas fornecidas por produtores que foram plantadas na terra preparada com material de compostagem. O objetivo foi a criação de uma horta orgânica e compostagem, possibilitando o aproveitamento de pequenos espaços e a reutilização de materiais que seriam descartados na natureza, capacitando os cuidadores, os adolescentes e os funcionários da instituição cientes das necessidades e desafios para construção de um planeta mais saudável, por meio de capacitação gratuita para a comunidade carente. A primeira colheita dos alimentos produzidos na horta ocorreu em junho quando começamos a utiliza-las no preparo dos alimentos que foram oferecidos as crianças, foram realizadas oficinas culinárias com as mães que passaram a incluir na dieta das crianças, além de iniciar o plantio doméstico. A horta orgânica e a oficina possibilitaram ampliar os conhecimentos e atuação das mães em relação ao plantio e a utilização das plantas com finalidades nutricionais na manutenção do bem-estar destas crianças.

The organic garden is based on clean and sustainable technologies without the use of any pesticides. Every day people are more and more concerned about their physical well-being, therefore they also try to feed themselves with healthier and natural products. The garden was set up in the premises of Rainha da Paz. We used organic seeds and plants supplied by producers and these were planted in prepared soil with compost material. The aim of this project was to create an organic garden and composting, allowing the use of small spaces and reuse of materials that would be discarded in nature. At the same time we will enable the teenagers and all the staff of the institution to be aware of the necessity and challenges in building a healthier planet, through free training of the disadvantaged community. The first harvest occurred in June, when we started to use the vegetables in the food preparation given to children. At the same time we offered cooking workshop to mothers, who started to include them in their diet and also initiated domestic planting. The organic garden and the workshop enabled mothers to broaden their knowledge and actions in relation to tillage and the use of plants for nutritional purposes to maintain the children's well-being.