Oviduct morphology and estrogen receptors ERα and ERß expression in captive Chinchilla lanigera (Hystricomorpha: Chinchillidae).

Chinchilla lanigera is a hystricomorph rodent from South America whose reproductive biology presents particular characteristics that distinguishes it from other Rodentia species, such as low reproductive rate, seasonal breeding pattern, and long estrous cycle. Nevertheless, reproductive features in female chinchillas are still poorly investigated, with a scarce knowledge concerning the estrous cycle and the histology of reproductive organs. In this study, we investigate the morphology, histomorphometry, secretory activity, and immunolocalization of estrogen receptors ERα and ERß in oviducts of nulliparous chinchillas, euthanized at fall season in Brazil. Follicular phase of estrous cycle of all studied animals was characterized by ovary and uterine morphology inspection, as well as vaginal cytology. Similar to other mammals, the oviduct wall of infundibulum, ampulla and isthmus was composed of mucosa, muscle, and serosa layers. Morphometric data of oviduct layers were used for identifying each oviduct segment. In the follicular phase, the oviduct was characterized by intense secretory activity, mainly in the ampulla, and expression of ERα and ERß throughout the oviduct epithelium. Both ERα and ERß were also detected in the connective tissue and smooth muscle cells. Our findings point out to the important role of estrogen in this female organ. Similar wide distribution of both ER proteins has been described for human Fallopian tube. Taken together, our data add to the understanding of the reproductive biology of female chinchillas, and may assist in the intensive breeding of this species and any eventual endeavor for conservation of chinchillas in the wild.

Técnica modificada de diafanizaçäo e coloraçäo diferencial de cartilagem e osso em pequenos vertebrados / A modified technique of diaphanisation and differential staining of cartilage and bone in small vertebrates

Sao apresentadas modificaçoes de técnicas usuais de coloraçao diferencial de osso e cartilagem de pequenos vertebrados, com Alizarina Red S e Alcian Blue, respectivamente, seguidos de diafanizaçao em soluçao de NaOH-glicerina. O resultado final da técnica modificada foi a intensa coloraçao de cartilagem em azul e de osso em vermelho. O tecido adiposo apresentou-se branco-leitoso e os demais tecidos moles transparentes.