Evaluation of the mandibular notch angle in three-dimensional virtual models as a parameter for determining age and sexual dimorphism / Avaliação do ângulo da incisura mandibular em modelos virtuais tridimensionais como parâmetro para estimativa de idade e dimorfismo sexual

Objective: The objective of this study was to analyze the opening of the mandibular notch angle (MNA) as a possible tool for predicting sex and estimating age so as to contribute to forensic anthropological studies. Material and Methods: For this, 60 cone beam computed tomographs (CBCT) belonging to the UNESP ICT Radiology archive, were selected: 5 female and 5 male for each decade of life, beginning on twenties until there seventies, totalizing 30 female and 30 male. Three-dimensional models were generated for each exam and measurements were performed with three-dimensional angular measuring tools using OnDemand 3D software (Cybermed Inc., Tustin, CA, USA) on the MNA. The MNA was measured considering the posteriormost portion of the coronoid process, the lowest point of the mandibular notch, and the anteriormost point of the condylar process of the mandible. The MannWhitney test was performed with the intention of establishing the possible comparative relationships between the values of angular measurements and sex. Results: No statistically significant differences were found between mandibular incision measurements in both sexes, as well as when comparing the angular measurements of the mandibular incisions and the different age groups studied, when a Kruskall-Wallis test was performed. Conclusion: It was concluded that the structure being evaluated did not appear to be an instrument capable of contributing to forensic anthropology evaluations. (Au)

Objetivo: O objetivo deste estudo foi analisar o ângulo de abertura da incisura mandibular (AIM) como uma possível ferramenta para predição sexual e estimativa de idade, de modo a contribuir com estudos antropológicos forenses. Materiais e Métodos: Para tanto, foram selecionadas 60 tomografias computadorizadas de feixe cônico (TCFC) pertencentes ao arquivo de Radiologia das ICT/UNESP: 30 do sexo feminino e 30 do sexo masculino, contendo 5 exames para cada sexo na 2ª, 3ª, 4ª, 5ª, 6ª e 7ª décadas de vida. Foram gerados modelos tridimensionais para cada exame e as medições foram realizadas com ferramentas de medição angular tridimensionais usando o software OnDemand 3D (Cybermed Inc., Tustin, CA, EUA) nos AIMs. O AIM foi medido considerando a porção mais posterior do processo coronoide, o ponto mais baixo da incisura mandibular, e o ponto mais anterior do processo condilar da mandíbula. O teste de Mann-Whitney foi realizado com o intuito de estabelecer as possíveis relações comparativas entre os valores das medidas angulares e sexo, que não foram encontradas diferenças estatisticamente significantes entre as medidas de incisão mandibular em ambos os sexos, assim como quando comparamos as medidas angulares da incisões mandibulares e as diferentes faixas etárias estudadas, foi realizado o teste de Kruskall-Wallis. Resultados: Em relação à reprodutibilidade das medidas, verificou-se que houve reprodutibilidade entre as medidas repetidas. Conclusão: Concluiu-se que a estrutura avaliada não parece ser um instrumento capaz de contribuir para as avaliações antropológicas forenses. (AU)

Delimitating cryptic species in the Gracilaria domingensis complex (Gracilariaceae, Rhodophyta) using molecular and morphological data.

Species in the genus Gracilaria that display conspicuously flattened vegetative morphologies are a taxonomically challenging group of marine benthic red algae. This is a result of their species richness, morphological similarity, and broad phenotypic plasticity. Within this group, the Gracilaria domingensis complex is one of the most common, conspicuous, and morphologically variable species along the tropical western Atlantic Ocean. Previous research has identified that members of this complex belong to two distantly related clades. However, despite this increased phylogentic resolution, species delimitations within each of these clades remain unclear. Our study assessed the species diversity within this difficult complex using morphological and molecular data from three genetic markers (cox1, UPA, and rbcL). We additionally applied six single-marker species delimitation methods (SDM: ABGD, GMYCs, GMYCm, SPN, bPTP, and PTP) to rbcL, which were largely in agreement regarding species delimitation. These results, combined with our analysis of morphology, indicate that the G. domingensis complex includes seven distinct species, each of which are not all most closely related: G. cervicornis; a ressurected G. ferox; G. apiculata subsp. apiculata; a new species, Gracilaria baiana sp. nov.; G. intermedia subsp. intermedia; G. venezuelensis; and G. domingensis sensu stricto, which includes the later heterotypic synonym, G. yoneshigueana. Our study demonstrates the value of multipronged strategies, including the use of both molecular and morphological approaches, to decipher cryptic species of red algae.

Phylogeny of Gracilariaceae (Rhodophyta): evidence from plastid and mitochondrial nucleotide sequences.

Gracilariaceae are mostly pantropical red algae and include ~230 species in seven genera. Infrafamilial classification of the group has long been based on reproductive characters, but previous phylogenies have shown that traditionally circumscribed groups are not monophyletic. We performed phylogenetic analyses using two plastid (universal plastid amplicon and rbcL) and one mitochondrial (cox1) loci from a greatly expanded number of taxa to better assess generic relationships and understand patterns of character distributions. Our analyses produce the most well-supported phylogeny of the family to date, and indicate that key characteristics of spermatangia and cystocarp type do not delineate genera as commonly suggested. Our results further indicate that Hydropuntia is not monophyletic. Given their morphological overlap with closely related members of Gracilaria, we propose that Hydropuntia be synonymized with the former. Our results additionally expand the known ranges of several Gracilariaceae species to include Brazil. Lastly, we demonstrate that the recently described Gracilaria yoneshigueana should be synonymized as G. domingensis based on morphological and molecular characters. These results demonstrate the utility of DNA barcoding for understanding poorly known and fragmentary materials of cryptic red algae.

Evaluation of impacts of climate change and local stressors on the biotechnological potential of marine macroalgae: a brief theoretical discussion of likely scenarios

Climate change can be associated with variations in the frequency and intensity of extreme temperatures and precipitation events on the local and regional scales. Along coastal areas, flooding associated with increased occupation has seriously impacted products and services generated by marine life, in particular the biotechnological potential that macroalgae hold. Therefore, this paper analyzes the available information on the taxonomy, ecology and physiology of macroalgae and discusses the impacts of climate change and local stress on the biotechnological potential of Brazilian macroalgae. Based on data compiled from a series of floristic and ecological works, we note the disappearance in some Brazilian regions of major groups of biotechnological interest. In some cases, the introduction of exotic species has been documented, as well as expansion of the distribution range of economically important species. We also verify an increase in the similarities between the Brazilian phycogeographic provinces, although they still remain different. It is possible that these changes have resulted from the warming of South Atlantic water, as observed for its surface in southeastern Brazilian, mainly during the winter. However, unplanned urbanization of coastal areas can also produce similar biodiversity losses, which requires efforts to generate long-term temporal data on the composition, community structure and physiology of macroalgae.

The impact of coastal urbanization on the structure of phytobenthic communities in southern Brazil.

The anthropogenic pressures on coastal areas represent important factors affecting local, regional, and even global patterns of distribution and abundance of benthic organisms. This report undertakes a comparative analysis of the community structure of rocky shore intertidal phytobenthos in both pristine like environments (PLE) and urbanized environments (UBE) in southern Brazil, characterizing variations on different spatial scales. Multivariate analysis of variance indicated that the PLE is characterized by a larger number of taxa and an increased occurrence of Rhodophyta species in relation to UBE. In contrast, UBE were dominated by opportunistic algae, such as Cladophora and Ulva spp. Significance tests further indicated higher species richness and Shannon-Wiener diversity on the PLE in relation to UBE. Here we provide data showing the magnitude of seaweed biodiversity loss and discuss direct and indirect consequences of unplanned urbanization on these communities.

GRACILARIOPSIS MCLACHLANII SP. NOV. AND GRACILARIOPSIS PERSICA SP. NOV. OF THE GRACILARIACEAE (GRACILARIALES, RHODOPHYCEAE) FROM THE INDIAN OCEAN(1).

Two new species of Gracilariopsis from the Indian Ocean are proposed-Gracilariopsis (Gp.) mclachlanii Buriyo, Bellorin et M. C. Oliveira sp. nov. from Tanzania and Gracilariopsis persica Bellorin, Sohrabipour et E. C. Oliveira sp. nov. from Iran-based on morphology and DNA sequence data (rbcL gene and SSU rDNA). Both species fit the typical features of Gracilariopsis: axes cylindrical throughout, freely and loosely ramified up to four orders, with an abrupt transition in cell size from medulla to cortex, cystocarps lacking tubular nutritive cells and superficial spermatangia. Nucleotide sequence comparisons of rbcL and SSU rDNA placed both species into the Gracilariopsis clade as distinct species from all the accepted species for this genus, forming a deeply divergent lineage together with some species from the Pacific. The new species are very difficult to distinguish on morphological grounds from other species of Gracilariopsis, stressing the importance of homologous molecular marker comparisons for the species recognition in this character-poor genus.

Decadal changes in a polluted bay as seen from its seaweed flora: the case of Santos Bay in Brazil.

The first comprehensive seaweed flora of a Brazilian region was published by Joly in 1957, describing and illustrating 105 taxa. Twenty years later Oliveira and Berchez resurveyed the area and found only 69 taxa, with a strong reduction in the number of brown algae. In another survey, performed in 1991 at the 3 studied stations with the largest species richness, the same reduction trend was found and no brown algae were observed. This reduction in biodiversity was attributed to increased pollution. Here we present the results of a new survey made in 1998-1999. This shows the return of some species recorded in the 1950s, including 7 species of brown algae. This increase in species number is thought to be a consequence of the efforts made by the local government to reduce pollution and the building of a submarine sewage terminal.

Identificaçäo e caracterizaçäo de manitol em Sargassum cymosum agardh, uma alga parda do litoral brasileiro / Identification and caracterization of mannitol in Sargassum cymosum agardh, a algae of brasilian coast

A ocorrência do manitol foi constatada nos extratos acetônico e etanólico de Sargassum cymosum (Phaeophyta) e sua identificaçäo realizada através de dados espectrais no infravermelho e comparaçäo direta com amostra padräo