Perfil metabólico de filhotes de peixe-boi da Amazônia (Trichechus inunguis) em cativeiro, alimentados com diferentes sucedâneos do leite materno / [Metabolic profile of captive Amazonian manatee calves (Trichechus inunguis), fed different substitutes for maternal milk]

Dez filhotes de peixe-boi da Amazônia criados em cativeiro foram amamentados com diferentes dietas lácteas em duas etapas. Na primeira, com sucedâneo 1 (S1), utilizado rotineiramente no Inpa e, na segunda, com sucedâneo 2 (S2), elaborado com base na composição centesimal do leite materno da espécie. Cada etapa durou 62 dias, realizando-se coleta de sangue ao final de cada uma para avaliação dos valores de proteínas totais (PT), albumina (ALB), globulinas (GLO), glicose (GLI), lipídeos totais (LT), colesterol (COL), triglicerídeos (TRI), cálcio (Ca), fósforo (P), magnésio (Mg), sódio (Na), potássio (K), hematócrito (Ht) e hemoglobina (Hb). Houve redução dos níveis séricos de PT, ALB, GLO, Ca, P, K, GLI, LT, COL e TRI, após a alimentação dos animais com S2 quando comparados aos valores obtidos com S1. Os níveis de Na foram maiores quando administrado o S2 (143,47±0,99mmol/L). Valores de Ht e Mg não diferiram entre os tratamentos. O perfil metabólico, em geral, esteve dentro dos níveis normais para a espécie.(AU)

Ten Amazonian manatee calves who live in captivity were fed with different substitutes for maternal milk in two phases. During the first phase, the calves were fed substitute 1 (S1), which is what was routinely used by INPA, and during the second phase they were fed substitute 2 (S2), which was formulated based on the nutritional composition of T. inunguis maternal milk. Each phase lasted 62 days, when blood samples were collected for analysis of values of total protein (PT), albumin (ALB), globulin (GLO), glucose (GLI), total lipids (LT), cholesterol (COL), triglycerides (TRI), calcium (Ca), phosphorus (P), magnesium (Mg), sodium (Na), potassium (K), hematocrit (Ht) and hemoglobin (Hb). There was a decrease in PT, ALB, GLO, Ca, P and K after feeding the animals with S2 when compared to values obtained with S1. Sodium levels were higher when S2 was administered (148.16±1.15mmol/L) than when S1 was given (143.47±0.99 mmol/l). Values of Ht and Mg did not differ between treatments. The metabolic profile was within normal levels for this species.(AU)

Evaluation of whole-body tumor burden with 68Ga-PSMA PET/CT in the biochemical recurrence of prostate cancer.

BACKGROUND: 68 Ga-PSMA-PET has an increasing importance in the evaluation of prostate cancer patients due to its high sensitivity and specificity in identifying neoplastic lesions in the clinical setting of elevated prostate-specific antigen (PSA). The objective of this study was to calculate the whole-body tumor burden using volumetric quantification of lesions detected in 68Ga-PSMA-PET of prostate cancer patients with biochemical recurrence and correlate these findings with clinical and image parameters. METHODS: Each patient had their 68Ga-PSMA-PET analyzed for the presence of neoplastic lesions. Their PSA levels and clinical information were recorded. In positive cases, the tumor burden (TL-PSMA) was calculated with a semi-automatic software and manually, and the results are analyzed and tested. RESULTS: We analyzed 100 prostate cancer patients, mean age of 69.9 ± 9.7 years and a median PSA of 1.73 ng/dL. 68Ga-PSMA-PET identified neoplastic lesions in 72% of them. The median TL-PSMA was 55.95 ml (1.1-28,080 ml). TL-PSMA and PSA were strongly correlated (rho = 0.71, p < 0.0001, 95% CI 0.60-0.80). TL-PSMA and PSA levels groups had a significant correlation and TL-PSMA and Gleason score were independent variables associated with PSA levels (p < 0.05). CONCLUSION: TL-PSMA strongly and independently correlates with PSA levels in prostate cancer patients and could be used as a biomarker to separate them into groups with high or low tumor burden, instead of considering only the number of lesions.