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Arch Virol ; 165(5): 1211-1214, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32170392

RESUMO

Papaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction.


Assuntos
Carica/virologia , Reação em Cadeia da Polimerase Multiplex/métodos , Doenças das Plantas/virologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Totiviridae/isolamento & purificação , Técnicas de Diagnóstico Molecular/métodos , Totiviridae/classificação , Totiviridae/genética
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