RESUMO
Os autores analisaram um lote de oito bezerros da raca Holandesa preta e branca, pertencentes a um mesmo plantel e criados em sistema de confinamento, que apresentavam um quadro de pneumonia. De quatro deles, foram isolados e caracterizados microrganismos do genero Mycoplasma atraves do cultivo "in vitro" e estudo da ultra estrutura eletronica, sendo uma das especies identificada como Mycoplasma bovis pelo "Mycoplasma Reference Laboratory-Colindale, England"
Assuntos
Doenças dos Bovinos , Mycoplasma , PneumoniaRESUMO
Using M. lepraemurium suspensions obtained from leproma of rats inoculated by subcutaneous route or from nodules of the peritoneal cavity, the AA. have tested the capacity of these bacilli in reduce the methylene blue and formation of formazan from the tetrazolium. The time or intensity of reduction of methylene blue or formation of formazan are proportional to the concentration of bacilli in the suspensions and is increased with the storage in the refrigerator at 1øC. In comparison with tests perfomed upon other acid fast bacilli the ones with M. lepraemurium are developed very slowly.
Assuntos
Animais , Ratos , Azul de Metileno , Azul de Metileno/síntese química , Mycobacterium lepraemurium/fisiologia , Mycobacterium lepraemurium/genética , Mycobacterium lepraemurium/isolamento & purificaçãoRESUMO
The AA. have made bactericidal studies of drug solution (promin, sulphetrone and sulfanilamide) at 40 miligrams per 100 ml. upon M. lepraemurium suspensions and using the method described in previous report, they tested the capacity of these bacillary suspensions in reduce the methylene blue. By these method they failed to show any bactericidal activity of these drugs on the M. lepraemurium.
