1.
Rev Hosp Clin Fac Med Sao Paulo
; 54(3): 73-4, 1999.
Artigo
em Inglês
| MEDLINE
| ID: mdl-10668275
2.
Acta Trop
; 57(4): 307-15, 1994 Sep.
Artigo
em Inglês
| MEDLINE
| ID: mdl-7810387
RESUMO
Trypanosoma cruzi trypomastigotes were shown to predominantly release high molecular weight components (above 50 kDa) when allowed to shed for 1 hour in protein-free media. Under these conditions, parasites were not damaged or lysed, as was indicated by: (a) their normal mobility; (b) their retaining of some of the labelled proteins; (c) the unchanged pattern of biotinylated surface proteins after shedding. Shed components were shown to display proteinase activities, detected at 97 and 50/60 kDa in gelatin gels. These proteolytic activities were completely inhibited by E-64, indicating that they were due to cysteine proteinases.