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1.
Planta ; 239(1): 243-54, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24162362

RESUMO

To advance our understanding of the formation of tension wood, we investigated the macromolecular arrangement in cell walls by Fourier transform infrared microspectroscopy (FTIR) during maturation of tension wood in poplar (Populus tremula x P. alba, clone INRA 717-1B4). The relation between changes in composition and the deposition of the G-layer in tension wood was analysed. Polarised FTIR measurements indicated that in tension wood, already before G-layer formation, a more ordered structure of carbohydrates at an angle more parallel to the fibre axis exists. This was clearly different from the behaviour of opposite wood. With the formation of the S2 layer in opposite wood and the G-layer in tension wood, the orientation signals from the amorphous carbohydrates like hemicelluloses and pectins were different between opposite wood and tension wood. For tension wood, the orientation for these bands remains the same all along the cell wall maturation process, probably reflecting a continued deposition of xyloglucan or xylan, with an orientation different to that in the S2 wall throughout the whole process. In tension wood, the lignin was more highly oriented in the S2 layer than in opposite wood.


Assuntos
Parede Celular/química , Populus/citologia , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Madeira/química , Madeira/crescimento & desenvolvimento , Glucanos/análise , Lignina/análise , Pectinas/análise , Polissacarídeos/análise , Populus/química , Xilanos/análise
2.
Planta ; 233(6): 1277-86, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21340698

RESUMO

Polarisation Fourier transform infra-red (FTIR) microspectroscopy was used to characterize the organisation and orientation of wood polymers in normal wood and tension wood from hybrid aspen (Populus tremula × Populus tremuloides). It is shown that both xylan and lignin in normal wood are highly oriented in the fibre wall. Their orientation is parallel with the cellulose microfibrils and hence in the direction of the fibre axis. In tension wood a similar orientation of lignin was found. However, in tension wood absorption peaks normally assigned to xylan exhibited a 90° change in the orientation dependence of the vibrations as compared with normal wood. The molecular origin of these vibrations are not known, but they are abundant enough to mask the orientation dependence of the xylan signal from the S2 layer in tension wood and could possibly come from other pentose sugars present in, or associated with, the gelatinous layer of tension wood fibres.


Assuntos
Parede Celular/ultraestrutura , Polímeros/metabolismo , Populus/ultraestrutura , Parede Celular/química , Parede Celular/metabolismo , Celulose/análise , Celulose/metabolismo , Imuno-Histoquímica , Lignina/análise , Lignina/metabolismo , Microfibrilas/química , Microfibrilas/metabolismo , Microscopia Eletrônica , Microscopia de Polarização/métodos , Polímeros/análise , Populus/química , Populus/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Estresse Mecânico , Madeira/anatomia & histologia
3.
Biomacromolecules ; 11(9): 2359-65, 2010 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-20831275

RESUMO

Several key enzymes in lignin biosynthesis of Populus have been down-regulated by transgenic approaches to investigate their role in wood lignification and to explore their potential for lignin modification. Cinnamate 4-hydroxylase is an enzyme in the early phenylpropanoid pathway that has not yet been functionally analyzed in Populus . This study shows that down-regulation of cinnamate 4-hydroxylase reduced Klason lignin content by 30% with no significant change in syringyl to guaiacyl ratio. The lignin reduction resulted in ultrastructural differences of the wood and a 10% decrease in wood density. Mechanical properties investigated by tensile tests and dynamic mechanical analysis showed a decrease in stiffness, which could be explained by the lower density. The study demonstrates that a large modification in lignin content only has minor influences on tensile properties of wood in its axial direction and highlights the usefulness of wood modified beyond its natural variation by transgene technology in exploring the impact of wood biopolymer composition and ultrastructure on its material properties.


Assuntos
Lignina/metabolismo , Plantas Geneticamente Modificadas/enzimologia , Plantas Geneticamente Modificadas/genética , Populus/enzimologia , Transcinamato 4-Mono-Oxigenase/genética , Madeira/química , Regulação para Baixo , Cromatografia Gasosa-Espectrometria de Massas , Microscopia de Força Atômica , Polissacarídeos/metabolismo , Populus/genética , Populus/ultraestrutura , RNA Mensageiro/genética , RNA de Plantas/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura , Resistência à Tração , Transcinamato 4-Mono-Oxigenase/análise , Transcinamato 4-Mono-Oxigenase/antagonistas & inibidores , Água/química , Madeira/genética , Madeira/ultraestrutura
4.
Carbohydr Res ; 339(4): 813-8, 2004 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-14980824

RESUMO

The nature of water sorption to different materials has always been a complex matter to address, partly due to the different possibilities of hydrogen-bond formation. For cellulosic materials this is extremely important for its product performance. In order to gain a deeper understanding of the moisture adsorption mechanisms of cellulose and hemicelluloses, the molecular interaction between moisture and paper and between moisture and some wood polymers was studied using FTIR spectroscopy under stable humid conditions. It was found that all the moisture-sorbing sites adsorbed moisture to the same relative degree, and that the rate of adsorption was the same for all these sites. It was also noticed that the moisture is adsorbed in the form of clusters. A direct relationship was found between the moisture weight gain and the increase in the absorbance peaks for humidities up to 50% relative humidity after which the moisture gain increased faster, a fact that still remains to be explained.


Assuntos
Celulose/química , Papel , Polissacarídeos/química , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Água/análise , Adsorção , Ar , Umidade , Madeira
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