Image Contrast and Spectral Transmission in Intraocular Lenses with Nd:YAG Pits.

PURPOSE: Neodymium yttrium aluminum garnet (Nd:YAG) laser capsulotomy is considered as safe and effective method in the treatment of posterior capsule opacification. Nevertheless, side effects are described. The incorrectly adjusted focus of the laser beam during the procedure can lead to so-called YAG-pits or YAG-shots. In this experimental study, we measured spectral transmission to evaluate the image contrast and analyze the impact of YAG-pits in intraocular lenses (IOL). METHODS: Acrylic, foldable, one-piece IOLs with 6.0 mm optic and different material properties were studied. These included: monofocal IOLs and enhanced monofocal IOLs with water content of 0.3%, 26.0%, and 4.0% and a refractive index of 1.49, 1.46, and 1.54, respectively. All measurements were done with new, unaltered IOLs and IOLs with YAG-pits. Damage was intentionally created, performing YAG-pits (n = 7) in the central zone (3.5 mm) using a photodisruption laser (2.0mJ). All laboratory measurements were repeated: These included surface topography characterization, United States Air Force (USAF) resolution test chart analysis, spectral transmittance measurements and through focus contrast measurement. RESULTS: Significant differences were found between the unaltered lenses and lenses with defects. The YAG-pits within the optic of the IOLs decreased the image contrast and spectral transmission and changed results of USAF test images at the focal position by 62%, 57% and 54%, respectively. In all IOLs a reduction of the relative intensity of total transmitted light was observed between 450 and 700 nm wavelength. CONCLUSION: This experimental study confirmed that the IOL image performance deteriorates with YAG-pits. The total intensity of transmitted light or transmittance (without scattering) was reduced in the wavelength between 450 and 700 nm. The contrast was significantly reduced and USAF test targets showed much worse results compared to unmodified counterparts. There was no systematic difference between monofocal and enhanced monofocal lenses. Further experiments should investigate the effect of YAG-pits on diffractive IOLs.

Evaluating impact of Nd: YAG laser associated defects on optical quality of hydrophilic and hydrophobic intraocular lenses using visualization of light propagation and USAF test targets.

BACKGROUND: Neodymium:yttrium aluminum garnet (Nd:YAG) laser capsulotomy is a well-accepted, safe, and effective measure in the treatment of posterior capsule opacification. However, iatrogenic intraocular lens damage is a relatively common side effect that happens due to inappropriate focusing during the procedure. This experimental study analyzes the impact of YAG-pits to obtain qualitative information. METHODS: Acrylic, monofocal hydrophilic and hydrophobic intraocular lenses (IOLs) with 6.0 mm optic and the with the same power (21D) were studied. First, all measurements were done with unmodified IOLs. Damage was intentionally created, performing YAG-pits (n = 5) in the central area of the lens optic (3.0 mm) using a photodisruption laser with the same energy level of 1.8 mJ. To simulate the cruciate pattern, the 5 defects were created in a cross shape within the 3.0 mm optical zone. Afterwards, all laboratory measurements were repeated: These included the United States Air Force (USAF) resolution test chart to study the imaging performance of the IOL, light field measurements to show the course of the rays behind the IOL and the modulation transfer function (MTF) measurements were analyzed. RESULTS: Evaluating USAF showed that unmodified lenses produced a sharper image. Damaged lenses led to a more blurred image and to the impression of a lower contrast with a kind of halo/glare effect. The light field measurement showed that YAG-pits led to a kind of dispersion and scattering effect, which was higher in hydrophobic IOLs. MTF showed a deterioration in damaged hydrophilic and hydrophobic IOLs, respectively. CONCLUSION: Our experimental study confirms that YAG-pits can reduce imaging quality of intraocular lenses. These defects behave as a new Huygens source, distribute a spherical wave that additionally illuminate the background of the USAF target. It can be assumed that material properties of the IOL (water content, refractive index) play an important role and affect results. The impact level is strongly dependent on the number, size and position of YAG-pits within the optic. LIMITATION: Only monofocal IOLs have been investigated so far, further tests with various IOL optics have to follow. In addition, simulating the circular pattern of YAG capsulotomy is necessary.

Investigating the feasibility of a hand-held photoacoustic imaging probe for margin assessment during breast conserving surgery.

Approximately 19 % of breast cancer patients undergoing breast conserving surgery (BCS) must return for a secondary surgery due to incomplete tumour removal. Our previous work demonstrated that the lower lipid content, characteristic of tumour tissue, was observed as regions of hypo-intense photoacoustic (PA) contrast. The goal of this work was to evaluate feasibility of a low-frequency, hand-held PA imaging probe for surgical margin assessment based on lipid content differences. Here, we describe (i) the design of a prototype hand-held PA imaging probe, (ii) the effect of limited-bandwidth on image contrast, (iii) accuracy towards hypo-intense contrast detection, (iv) the limited-view characteristics of the single sensor design, and (iv) early imaging results of an ex-vivo breast cancer specimen. The probe incorporates a single polyvinylidene fluoride acoustic sensor, a 1-to-4 optical fibre bundle and a polycarbonate axicon lens for light delivery. Imaging results on phantoms designed to mimic positive margins demonstrated the ability to detect gaps in optical absorption as small as 1 mm in width. Compared to images from a near full-view PAI system, the hand-held PAI probe had higher signal to noise ratio but suffered from negativity image artifacts. Lumpectomy specimen imaging showed that strong signals can be obtained from the fatty tissue. Taken together, the results show this imaging approach with a hand-held probe has potential for detection of residual breast cancer tissue during BCS; however, more work is needed to reduce the size of the probe to fit within the surgical cavity.

PATLAB: A graphical computational software package for photoacoustic computed tomography research.

Photoacoustic tomography (PAT) provides high resolution optical images of tissue at depths of up to several centimetres. This modality has been of interest to researchers for at least 30 years and is still the subject of intensive research. However, PAT researchers lack access to a comprehensive open-source graphical simulation and reconstruction software package. In this article, we introduce PATLAB, an open-source MATLAB-based graphical software package that can perform both PAT simulation and image reconstruction. PATLAB is simple to use yet is capable of complex PAT data processing tasks and offers advanced users a framework to build and test new methods.