RESUMO
We evaluated bronopol as a practical alternative anti-fungal agent to malachite green for use in hatcheries. Repeated exposure everyday, just after fertilization to the stage of eggs showing eye development to 50 mg/L and 100 mg/L bronopol for 30 min showed an efficacy comparable to 0 mg/L (control) for the inhibition of fungal infection in rainbow trout (Oncorhynchus mykiss) eggs. The 100 mg/L bronopol treatment groups showed a control of fungal infection up to the stage of eye development in eggs. Significant differences in terms of the number of eggs showing eye development were seen between 0 mg/L and treatment at 50 and 100 mg/L bronopol.
Assuntos
Antifúngicos/uso terapêutico , Doenças dos Peixes/microbiologia , Doenças dos Peixes/prevenção & controle , Fungos/crescimento & desenvolvimento , Micoses/veterinária , Oncorhynchus mykiss , Propilenoglicóis/uso terapêutico , Animais , Aquicultura/métodos , Feminino , Micoses/microbiologia , Micoses/prevenção & controleRESUMO
This study compared the antifungal activities of bronopol and 2-methyl-4-isothiazolin-3-one (MT) against members of the genus Saprolegnia in the family saprolegniaceae, which have the ability to infect fish. The minimum inhibitory concentration of bronopol was 100-200 mg/l but for MT it was 31 mg/l. Concerning fungicidal effects against the hyphae, treatment with bronopol at 100-200 mg/l for 30 min or treatment with MT at 25-50 mg/l for 30 min was effective in killing the vegetative stage of all fungal strains tested. Treatment with bronopol at 100 mg/l for 60 min or treatment with MT at 25-50 mg/l for 60 min was effective in killing the zoospores of all fungal strains tested. These results showed that MT was a more effective antifungal agent than bronopol against infectious members of the genus Saprolegnia.
