RESUMO
In a 21-year-old woman, suffering from a moderate painful erythematous infiltrated lesion in the neck, cowpox was diagnosed. Contact with an infected cat was suggested. Healing was spontaneous.
Assuntos
Técnicos em Manejo de Animais , Vírus da Varíola Bovina/isolamento & purificação , Varíola Bovina/diagnóstico , Doenças Profissionais/diagnóstico , Zoonoses , Adulto , Animais , Gatos , Feminino , Humanos , Exposição Ocupacional , Reação em Cadeia da PolimeraseRESUMO
We have identified a MAR/SAR recognition signature (MRS) which is common to a large group of matrix and scaffold attachment regions. The MRS is composed of two degenerate sequences (AATAAYAA and AWWRTAANNWWGNNNC) within close proximity. Analysis of >300 kb of genomic sequence from a variety of eukaryotic organisms shows that the MRS faithfully predicts 80% of MARs and SARs. In each case where we find a MRS, the corresponding DNA region binds specifically to the nuclear scaffold. Although all MRSs are associated with a SAR, not all known SARs and MARs contain a MRS, suggesting that at least two classes exist, one containing a MRS, the other not. Evidence is presented that the two sequence elements of the bipartite MRS occupy a position on the nucleosome near the dyad axis, together creating a putative protein binding site. The identification of a MAR- and SAR-associated DNA element is an important step forward towards understanding the molecular mechanisms of these elements. It will allow: (i) analysis of the genomic location of SARs, e.g. in relationship to genes, based on sequence information alone, rather than on the basis of an elaborate biochemical assay; (ii) identification and analysis of proteins that specifically bind to the MRS.
Assuntos
Proteínas de Ligação a DNA/metabolismo , DNA/metabolismo , Matriz Nuclear/metabolismo , Proteínas Nucleares/metabolismo , Sequências Reguladoras de Ácido Nucleico/genética , Animais , Antígenos Nucleares , Arabidopsis/genética , Sequência de Bases , Sítios de Ligação , Caenorhabditis elegans/genética , Núcleo Celular/genética , Núcleo Celular/metabolismo , Sequência Conservada/genética , DNA/genética , Células Eucarióticas/citologia , Células Eucarióticas/metabolismo , Genoma , Globinas/genética , Humanos , Região de Controle de Locus Gênico/genética , Nucleossomos/genética , Nucleossomos/metabolismoRESUMO
The Arabidopsis thaliana genome is currently being sequenced, eventually leading towards the unravelling of all potential genes. We wanted to gain more insight into the way this genome might be organized at the ultrastructural level. To this extent we identified matrix attachment regions demarking potential chromatin domains, in a 16 kb region around the plastocyanin gene. The region was cloned and sequenced revealing six genes in addition to the plastocyanin gene. Using an heterologous in vitro nuclear matrix binding assay, to search for evolutionary conserved matrix attachment regions (MARs), we identified three such MARs. These three MARs divide the region into two small chromatin domains of 5 kb, each containing two genes. Comparison of the sequence of the three MARs revealed a degenerated 21 bp sequence that is shared between these MARs and that is not found elsewhere in the region. A similar sequence element is also present in four other MARs of Arabidopsis.Therefore, this sequence may constitute a landmark for the position of MARs in the genome of this plant. In a genomic sequence database of Arabidopsis the 21 bp element is found approximately once every 10 kb. The compactness of the Arabidopsis genome could account for the high incidence of MARs and MRSs we observed.
Assuntos
Arabidopsis/genética , Cromatina/genética , Genes de Plantas , Plastocianina/genética , Sequência de Aminoácidos , Sequência de Bases , Mapeamento Cromossômico , Clonagem Molecular , Sequência Consenso , DNA de Plantas/genética , Evolução Molecular , Genoma de Planta , Matriz Nuclear/genética , Sequências Repetitivas de Ácido NucleicoRESUMO
The 5'-terminal and 3'-terminal parts of the single open reading frame (ORF) in the L RNA of tomato spotted wilt virus (TSWV) were expressed using a prokaryotic expression system. Using antibodies raised against the translational products obtained a 330-kDa protein could be specifically detected in preparations of purified virions and in nucleocapsid preparations from TSWV-infected leaf tissue. The results obtained indicate that the L protein of TSWV, though much larger than that of the animal-infecting bunyaviruses, is present in virus particles in an unprocessed, intact form.
Assuntos
RNA Viral/metabolismo , Tospovirus/metabolismo , Proteínas Virais/biossíntese , Sequência de Bases , Clonagem Molecular , Primers do DNA , Escherichia coli , Dados de Sequência Molecular , Fases de Leitura Aberta , RNA Viral/análise , Tospovirus/genética , Proteínas Virais/análiseAssuntos
Prurido Anal/terapia , Terapia Combinada , Feminino , Humanos , Masculino , Prurido Anal/diagnóstico , Prurido Anal/etiologiaAssuntos
Doenças da Unha/etiologia , Unhas/patologia , Adolescente , Atrofia , Humanos , Masculino , SíndromeRESUMO
Impetigo herpetiformis was diagnosed in a primigravida woman on clinical, laboratory, and histopathological grounds. This disease should be considered as a rare and serious variant of generalized pustular psoriasis. After initiating prednisone therapy, antepartum registration of fetal heart rate patterns appears necessary to evaluate fetal well-being. Subsequent pregnancies of the patient were not recommended.