Monitoring iohexol and its transformation products as evidence of reclaimed water irrigation input to contiguous waterbodies.

Irrigation with reclaimed water alleviates water supply shortages, but excess application often results in impairment of contiguous waterbodies. This project investigated the potential use of iohexol, an iodinated contrast media used in medical imaging, together with its bio- and phototransformation products as unique reconnaissance markers of reclaimed water irrigation intrusion at three golf courses within the state of Florida. Inter-facility iohexol concentrations measured in reclaimed waters ranged over ~2 orders of magnitude while observed intra-facility seasonal differences were ≤1 order of magnitude. A ~50 % reduction in iohexol was observed post-disinfection for reclaimed water facilities utilizing UV light while none was observed with use of chlorine. Iohexol biotransformation products were observed to decline or shift to lower molecular weight compounds when exposed to UV light but not during disinfection using chlorine. Iohexol biotransformation products were observed in most of the samples but were more prevalent in samples collected during the dry season. Much fewer iohexol phototransformation products were observed in chlorinated reclaimed water, and they were only observed in UV light irradiated reclaimed water when the pre-disinfectant iohexol concentration was ≥5000 ng/L or from solar exposure of reclaimed water spiked with 10 µM of iohexol. For the Hillsborough golf course overlaying an aquifer, the groundwater did not contain iohexol or phototransformation products but did contain biotransformation products. It is not known if these biotransformation products are from active or historical intrusion. The additional presence of sucralose in the aquifer suggests that intrusion has occurred within the past 3 years. This study demonstrates three crucial points in attempting to utilize iohexol to denote reclaimed water intrusion from irrigation overapplication: (1) interpretable results are obtained when iohexol concentrations in the reclaimed water employed for irrigation are ≥1000 ng/L, with higher concentrations in the range of ≥5000 ng/L better able to meet analytical sensitivity requirements after further dilution or degradation in the environment; (2) it is beneficial to assess iohexol transformation products in tandem with iohexol monitoring to account for environmental transformations of iohexol during storage and transport to the receiving water of concern; and (3) inclusion of monitoring for sucralose, an artificial sweetener ubiquitous in wastewater sources that is comparatively stable in the environment, can aid in interpretating whether reclaimed water intrusion based on identification of iohexol and transformation products in the receiving water is attributable to historic or ongoing irrigation overapplications.

Impact of environmental conditions on the suitability of microconstituents as markers for determining nutrient loading from reclaimed water.

Nitrogen and phosphorous loading into waterways from designated beneficial uses of reclaimed water is a growing concern in many parts of the United States. Numerous studies have documented that organic microconstituents present in the reclaimed water can be utilized as indicators of its influence on surface water bodies. However, little to no information is available on the environmental attenuation of these microconstituents relative to the nutrients, which is a critical component in determining the effectiveness or limitations of those markers as a tool for elucidating their origins. In this study, the stability of selected markers (sucralose, carbamazepine, gadolinium anomaly, iohexol, and atenolol) was evaluated through bench-scale studies designed to simulate environmental conditions associated with biodegradation, adsorption, and photolysis. The primary pathway for nitrogen reduction was biodegradation (greater than 99%) while the highest phosphorous removal was due to adsorption (30-80%). Soils with low organic content were selected for this study. Sucralose was the most recalcitrant microconstituent in the environment with less than 15% removal by adsorption, biodegradation, or photolysis. Iohexol was too susceptible to photolysis (90% removal), and atenolol was susceptible to biodegradation (60-80% removal). Gd anomaly was fairly stable (less than 30% removal) in the environment. Carbamazepine was another efficacious marker for wastewater, but was susceptible (50% removal) to photolysis. Of the selected microconstituents, only atenolol showed any similarity with the attenuation observed for nitrate and none of the microconstituents showed any similarity with the attenuation observed for phosphorus.

Differentiating sources of anthropogenic loading to impaired water bodies utilizing ratios of sucralose and other microconstituents.

Previous studies have suggested the use of sucralose, a synthetic non-nutritive sweetener, as an indicator of domestic wastewater loading to surface waters. This paper presents a novel flow schematic approach for quantifying volumetric load contributions from different water sources by utilizing sucralose as a master diagnostic variable in combination with other trace compounds. This conceptual approach was validated through demonstration of sucralose presence at positive field sites susceptible to either water reuse or septic infiltration and its absence at negative field sites. Differences in the ratios of carbamazepine to sucralose and gadolinium anomaly to sucralose were demonstrated for eight septic and water reuse effluents. Utilization of these ratios as a means of distinguishing septic and water reuse loading to water bodies merits additional study. In the absence of sustained loading, the use of carbamazepine might be hindered by photolysis and gadolinium anomaly might be hindered when volumetric loading is less than 20%.