RESUMO
The technique of countercurrent immunoelectrophoresis (CI), using the N-acetyl glucosamine-binding lectin from Helix pomatia, provided a rapid, sensitive, inexpensive, specific and reliable method for assaying blood group A-like substances in both bacterial and viral vaccines. Blood group A-like substance was detected in the pneumococcal polysaccharide vaccine manufactured by Merck Sharp & Dohme up to 1981 and in a staphylococcus vaccine ( Staphage Lysate) manufactured by Delmont Laboratories. Other US licensed vaccines, including diphtheria and tetanus toxoids, pertussis, meningococcal polysaccharide and influenza vaccines, did not contain detectable amounts of this substance. Human anti-A globulins did not provide a satisfactory reagent for the CI assay because they contained precipitating activities to the vaccine components.
Assuntos
Sistema ABO de Grupos Sanguíneos , Vacinas Bacterianas/análise , Lectinas/análise , Vacinas Virais/análise , Vacinas contra Cólera/análise , Contraimunoeletroforese , Toxoide Diftérico/análise , Humanos , Vacinas contra Influenza/análise , Neisseria meningitidis/imunologia , Vacina contra Coqueluche/análise , Streptococcus pneumoniae/imunologia , Toxoide Tetânico/análiseRESUMO
Type 7F and type 14 pneumococcal capsular polysaccharides, neutral at pH 8.6, were studied by immunoelectrophoresis at pH 5. Three techniques were used: rocket, countercurrent, and reversed immunoelectrophoresis. Our results show that these two capsular polysaccharides types can be detected at pH 5 with high sensitivity.
