[Biosynthesis of oleandomycin by cultures of Streptomyces antibioticus obtained after regeneration and fusion of protoplasts]. / Biosintez oleandomitsina kul'turami Streptomyces antibioticus, poluchennymi posle regeneratsii i sliianiia protoplastov.

The ability of Streptomyces antibioticus strains to synthesize oleandomycin is studied under the effect of regeneration and fusion of protoplasts. The production of strains-regenerants with an increased (by 30-50%) synthesis of oleandomycin is possible. Regenerants of mutants resistant to the proper antibiotic retain a high level of the oleandomycin synthesis more stably. Variations in the antibiotic-production ability are considered in regenerant populations of various generations.

[Effect of conditions of agitation and aeration on the biosynthesis of oleandomycin]. / Issledovanie vliianiia uslovii peremeshivaniia i aératsii na biosintez oleandomitsina.

The data on the effect of the aeration and agitation conditions on biosynthesis of oleandomycin in 0.75, 3 and 50 m3 apparatus are presented. The relationship between the biosynthetic parameters, oxygen dissolution rate and specific power consumption for agitation was studied. It was shown that the values of the specific power consumption for agitation were not acceptable for scaling up the process of oleandomycin biosynthesis.

[The effect of aeration and agitation on tetracycline biosynthesis]. / Vliianie uslovii aératsii i peremeshivaniia na biosintez tetratsiklina.

The results of the study on relation between tetracycline biosynthesis and the specific power input for agitation in pilot plant apparatus was studied. No correlation was observed between the levels of tetracycline biosynthesis and changes in the specific power input within a range of 0.6 to 2.3 kW/m3 at the expense of changes in the mixer diameter and the agitation rate, when the aeration rate was constant. It was shown that the aeration conditions were most significant for tetracycline biosynthesis. The study provided determination of the optimal aeration conditions for biosynthesis of tetracycline.

[Aeration conditions in the process of tetracycline biosynthesis. The role of organic acids]. / Izuchenie uslovii aératsii v protsesse biosinteza tetratsiklina. Rol' organicheskikh kislot.

The effect of the aeration conditions on the content of volatile acids in the fermentation broth was studied. It was shown that deterioration of the aeration conditions during the process of biosynthesis in both flasks and 750 1 fermentors resulted in decreased levels of the antibiotic accumulation and was accompanied by a simultaneous increase in the concentration of the volatile acids in the culture fluid. Under unfavourable aeration conditions the volatile acids present in the fermentation broth in higher concentrations than under the optimal conditions had no effect. It was shown that the volatile acid concentration may be used as a parameter for the control of the aeration conditions and as an index of normal biosynthetic process.