Genetic characterization of grapevine-infecting Botrytis cinerea isolates from Argentina

Antecedentes Botrytis cinerea es un ascomiceto con una gran diversidad genética y una compleja estructura poblacional cuya presencia ha sido descrita en diversos lugares y huéspedes distintos, pero nada se sabe acerca de su diversidad genética en Argentina. Objetivos El objetivo de este trabajo fue estimar la diversidad genética de una población local de aislamientos de B. cinerea obtenidos de vid en Argentina. Métodos En este trabajo, 35 cepas aisladas de vides fueron genotipadas mediante marcadores moleculares basados en PCR-RFLP y según la presencia de elementos transposables. Estos resultados fueron comparados con los de una gran población francesa del hongo, y utilizados para realizar un análisis de genética poblacional utilizando el software Genepop. Resultados Todos los aislamientos analizados fueron clasificados como grupo II (de acuerdo a la clasificación más recientemente propuesta) y mostraron un alto grado de diversidad genética, con 14 haplotipos distintos en el número de muestras involucradas. Se observó una notable diferencia en la frecuencia alélica entre ambas poblaciones. Conclusiones Estas diferencias entre las poblaciones comparadas condujeron a la detección de un alto nivel de diversidad y diferenciación poblacional entre los aislamientos locales y los franceses. Esto fue confirmado por un valor Fst de 0,3332, superior al previamente reportado para otras comparaciones de este tipo. Este trabajo es el primero en documentar la diversidad genética de aislamientos de B. cinerea y su estructura poblacional en Argentina(AU)

Background Botrytis cinerea is an ascomycete with a high genetic diversity and complex population structure, as reported from several hosts and sites. However, nothing is known about its genetic diversity in Argentina. Aims The aim of this work is to estimate the genetic diversity of a local population of B. cinerea isolates obtained from grapevine in Argentina. Methods In this work, 35 strains that had been isolated from grapevines were genotyped for the presence of transposable elements and PCR-based RFLP molecular markers. The obtained results were compared with those from a large French population of the fungus, and used to perform a population genetics analysis using the Genepop software. Results All the analysed isolates were classified as Group II (according to the most recent proposed classification) and showed a high degree of genetic diversity, with 14 different haplotypes. A significant difference in allele frequency was recorded between the local and French populations. Conclusions These comparisons between fungal populations, led to the detection of a high level of diversity and the differentiation between local and French groups of isolates. This was confirmed by an Fst value of 0.3332, which was higher than that reported for other pairwise comparisons of populations. This work constitutes the first report on the genetic diversity of B. cinerea isolates and their population structure in Argentina(AU)

Genetic characterization of grapevine-infecting Botrytis cinerea isolates from Argentina.

BACKGROUND: Botrytis cinerea is an ascomycete with a high genetic diversity and complex population structure, as reported from several hosts and sites. However, nothing is known about its genetic diversity in Argentina. AIMS: The aim of this work is to estimate the genetic diversity of a local population of B. cinerea isolates obtained from grapevine in Argentina. METHODS: In this work, 35 strains that had been isolated from grapevines were genotyped for the presence of transposable elements and PCR-based RFLP molecular markers. The obtained results were compared with those from a large French population of the fungus, and used to perform a population genetics analysis using the Genepop software. RESULTS: All the analysed isolates were classified as Group II (according to the most recent proposed classification) and showed a high degree of genetic diversity, with 14 different haplotypes. A significant difference in allele frequency was recorded between the local and French populations. CONCLUSIONS: These comparisons between fungal populations, led to the detection of a high level of diversity and the differentiation between local and French groups of isolates. This was confirmed by an Fst value of 0.3332, which was higher than that reported for other pairwise comparisons of populations. This work constitutes the first report on the genetic diversity of B. cinerea isolates and their population structure in Argentina.