Correction: Penagos-Tabares et al. Mixtures of Mycotoxins, Phytoestrogens, and Other Secondary Metabolites in Whole-Plant Corn Silages and Total Mixed Rations of Dairy Farms in Central and Northern Mexico. Toxins 2023, 15, 153.

In the original publication [...].

Mixtures of Mycotoxins, Phytoestrogens, and Other Secondary Metabolites in Whole-Plant Corn Silages and Total Mixed Rations of Dairy Farms in Central and Northern Mexico.

Mycotoxins and endocrine disruptors such as phytoestrogens can affect cattle health, reproduction, and productivity. Most studies of mycotoxins in dairy feeds in Mexico and worldwide have been focused on a few (regulated) mycotoxins. In contrast, less known fungal toxins, phytoestrogens, and other metabolites have been neglected and underestimated. This study analyzed a broad spectrum (>800) of mycotoxins, phytoestrogens, and fungal, plant, and unspecific secondary metabolites in whole-plant corn silages (WPCSs) and total mixed rations (TMRs) collected from 19 Mexican dairy farms. A validated multi-metabolite liquid chromatography/electrospray ionization-tandem mass spectrometric (LC/ESI-MS/MS) method was used. Our results revealed 125 of >800 tested (potentially toxic) secondary metabolites. WPCSs/TMRs in Mexico presented ubiquitous contamination with mycotoxins, phytoestrogens, and other metabolites. The average number of mycotoxins per TMR was 24, ranging from 9 to 31. Fusarium-derived secondary metabolites showed the highest frequencies, concentrations, and diversity among the detected fungal compounds. The most frequently detected mycotoxins in TMRs were zearalenone (ZEN) (100%), fumonisin B1 (FB1) (84%), and deoxynivalenol (84%). Aflatoxin B1 (AFB1) and ochratoxin A (OTA), previously reported in Mexico, were not detected. All TMR samples tested positive for phytoestrogens. Among the investigated dietary ingredients, corn stover, sorghum silage, and concentrate proportions were the most correlated with levels of total mycotoxins, fumonisins (Fs), and ergot alkaloids, respectively.

MERS-CoV and SARS-CoV infections in animals: a systematic review and meta-analysis of prevalence studies.

INTRODUCTION: Coronaviruses are zoonotic viruses that include human epidemic pathogens such as the Middle East Respiratory Syndrome virus (MERS-CoV), and the Severe Acute Respiratory Syndrome virus (SARS-CoV), among others (e.g., COVID-19, the recently emerging coronavirus disease). The role of animals as potential reservoirs for such pathogens remains an unanswered question. No systematic reviews have been published on this topic to date. METHODS: We performed a systematic literature review with meta-analysis, using three databases to assess MERS-CoV and SARS-CoV infection in animals and its diagnosis by serological and molecular tests. We performed a random-effects model meta-analysis to calculate the pooled prevalence and 95% confidence interval (95%CI). RESULTS: 6,493articles were retrieved (1960-2019). After screening by abstract/title, 50 articles were selected for full-text assessment. Of them, 42 were finally included for qualitative and quantitative analyses. From a total of 34 studies (n=20,896 animals), the pool prevalence by RT-PCR for MERS-CoV was 7.2% (95%CI 5.6-8.7%), with 97.3% occurring in camels, in which pool prevalence was 10.3% (95%CI 8.3-12.3). Qatar was the country with the highest MERS-CoV RT-PCR pool prevalence: 32.6% (95%CI 4.8-60.4%). From 5 studies and 2,618 animals, for SARS-CoV, the RT-PCR pool prevalence was 2.3% (95%CI 1.3-3.3). Of those, 38.35% were reported on bats, in which the pool prevalence was 14.1% (95%CI0.0-44.6%). DISCUSSION: A considerable proportion of infected animals tested positive, particularly by nucleic acid amplification tests (NAAT). This essential condition highlights the relevance of individual animals as reservoirs of MERS-CoV and SARS-CoV. In this meta-analysis, camels and bats were found to be positive by RT-PCR in over 10% of the cases for both; thus, suggesting their relevance in the maintenance of wild zoonotic transmission.

Comportamientos sexuales en mayores de 18 años con diagnóstico VIH/SIDA en tres ciudades de Colombia 2011 / Comportamentos sexuais em mais de 18 anos com diagnóstico de HIV / AIDS em três cidades na Colômbia 2011 / Sexual behaviors in over 18 years old with a diagnosis of HIV/AIDS in three cities of Colombia 2011

Objetivo: Describir los comportamientos sexuales en personas VIH positivas en tres ciudades de Colombia antes y después de conocer el diagnóstico e identificar cambios en estos comportamientos. Metodología: Estudio descriptivo, de corte trasversal, en el que participaron 85 individuos de tres ciudades colombianas, entrevistados durante el año 2011. Se cumplió con los parámetros éticos para investigaciones con personas. Para el análisis estadístico se utilizó el software SPSS 18.0. Resultados: Se encontraron diferencias significativas al comparar el comportamiento sexual antes y después de conocer el diagnóstico VIH positivo. el 23% de los entrevistados manifestó no haber iniciado actividades sexuales después del diagnóstico, el 62.5% empezó a usar el condón en todas sus relaciones sexuales. Las relaciones sexuales con personas VIH positivo aumentaron a un 35.4%. Las infecciones de transmisión sexual disminuyeron a un 12.3%. El consumo de alcohol previo al inicio de relaciones sexuales disminuyó a un 31.8%. Conclusiones: Se encontraron cambios en los comportamientos de riesgo de las personas después de recibir el diagnóstico; sin embargo, dichas modificaciones no son suficientes por tratarse de la trasmisión de una infección mortal y de elevados costos socioeconómicos.

Objective: To describe sexual behaviors of HIV positive patients in three cities of Colombia, before and after learning diagnosis and to identify changes. Methodology: Quantitative, descriptive, cross-sectional study, with participation of 85 individuals from three cities of Colombia who were interviewed during 2011. Ethical standards for research involving individuals were met during the study. The SPSS software version 18.0 was used for statistical analysis. Results: Significant differences were found when comparing their sexual behavior before and after knowing HIV positive diagnosis, 23.0% of respondents said that they had not resumed sexual activity, 62.5% of the patients began to use the condom in all sexual relations. Sex with HIV-positive people increased a 35.4%. Sexually transmitted infections were reduced to a 12.3%. The consumption of alcohol prior to the onset of sexual intercourse decreased to 31.8%. Conclusions: Changes were found regarding risky behaviors of people after knowing the diagnosis; however, such modifications are not enough regarding the transmission of a deadly infection and with high social and economic costs.

Objetivo: Descrever o comportamento sexual em pessoas HIV-positivas em três cidades na Colômbia antes e depois de conhecer o diagnóstico e identificar mudanças no esses comportamentos. Metodologia: Estudo descritivo de transversal, em que 85 indivíduos participaram em três cidades colombianas, entrevistado em 2011. Eles se conheceram os padrões éticos para a investigação com as pessoas. Para análise estatística foi utilizado SPSS 18.0 software. Resultados: Diferenças significativas na comparação entre o comportamento sexual foram encontrados antes e depois de aprender sobre o diagnóstico de HIV positivo. 23% dos entrevistados disseram que a atividade sexual não ter iniciado após o diagnóstico, 62,5% começaram a usar preservativos em todas as relações sexuais. Sexo com pessoas HIV positivas aumentou para 35,4%. infecções sexualmente transmissíveis diminuiu para 12,3%. O consumo de álcool antes do início do sexo diminuiu para 31,8%. Conclusões: foram encontradas alterações nos comportamentos de risco de pessoas depois de receber o diagnóstico; No entanto, estas alterações não são suficientes, porque é a transmissão de uma infecção fatal e custos socioeconómicos elevados.