RESUMO
Improving the imaging speed of multiphoton microscopy is an active research field. Among recent strategies, light-sheet illumination holds distinctive advantages for achieving fast imaging in vivo. However, photoperturbation in multiphoton light-sheet microscopy remains poorly investigated. We show here that the heart beat rate of zebrafish embryos is a sensitive probe of linear and nonlinear photoperturbations. By analyzing its behavior with respect to laser power, pulse frequency and wavelength, we derive guidelines to find the best balance between signal and photoperturbation. We then demonstrate one order-of-magnitude signal enhancement over previous implementations by optimizing the laser pulse frequency. These results open new opportunities for fast live tissue imaging.
RESUMO
We report the implementation of a high-rate source of single- and two-photon states. By combining the advantages of short pulses and cavities, heralding rates as high as 200 kHz have been obtained for the single photons, as well as 250 Hz for the two-photon states. In this setup, homodyne measurements are conditioned by the heralding of the quantum states thanks to the introduction of a low-loss optical delay line in the heralded states path. This enables the detection of most of the heralded events, and fidelities reaching 68.5% (91% with correction for detection efficiency) and 50.4% (85% with correction) were obtained for the single- and two-photon states, respectively. Such high rates and fidelities in the generation of elementary Fock states may open the path for the production of complex quantum states.
