Increased nuclear beta-catenin expression in oral potentially malignant lesions: A marker of epithelial dysplasia

BACKGROUND: Deregulation of beta-catenin is associated with malignant transformation; however, its relationship with potentially malignant and malignant oral processes is not fully understood. The aim of this study was to determine and compare the nuclear beta-catenin expression in oral dysplasia and oral squamous cell carcinoma (OSCC). MATERIAL AND METHODS: Cross sectional study. Immunodetection of beta-catenin was performed on 72 samples, with the following distribution: 21 mild dysplasia, 12 moderate dysplasia, severe dysplasia 3, 36 OSCC including 19 well differentiated, 15 moderately differentiated and 2 poorly differentiated. Through microscopic observation the number of positive cells per 1000 epithelial cells was counted. For the statistical analysis, the Kruskal Wallis test was used. RESULTS: Nuclear expression of beta-catenin was observed in all samples with severe and moderate dysplasia, with a median of 267.5, in comparison to mild dysplasia whose median was 103.75. Only 10 samples (27.7%) with OSCC showed nuclear expression, with statistically significant differences between groups (p < 0.05). CONCLUSIONS: Our results are consistent with most of the reports which show increased presence of beta-catenin in severe and moderate dysplasia compared to mild dysplasia; however the expression of nuclear beta-catenin decreased after starting the invasive neoplastic process. This suggests a role for this protein in the progression of dysplasia and early malignant transformation to OSCC. Immunodetection of beta-catenin could be a possible immune marker in the detection of oral displasia

Increased nuclear ß-catenin expression in oral potentially malignant lesions: A marker of epithelial dysplasia.

BACKGROUND: Deregulation of ß-catenin is associated with malignant transformation; however, its relationship with potentially malignant and malignant oral processes is not fully understood. The aim of this study was to determine and compare the nuclear ß-catenin expression in oral dysplasia and oral squamous cell carcinoma (OSCC). MATERIAL AND METHODS: Cross sectional study. Immunodetection of ß-catenin was performed on 72 samples, with the following distribution: 21 mild dysplasia, 12 moderate dysplasia, severe dysplasia 3, 36 OSCC including 19 well differentiated, 15 moderately differentiated and 2 poorly differentiated. Through microscopic observation the number of positive cells per 1000 epithelial cells was counted. For the statistical analysis, the Kruskal Wallis test was used. RESULTS: Nuclear expression of ß-catenin was observed in all samples with severe and moderate dysplasia, with a median of 267.5, in comparison to mild dysplasia whose median was 103.75. Only 10 samples (27.7%) with OSCC showed nuclear expression, with statistically significant differences between groups (p < 0.05). CONCLUSIONS: Our results are consistent with most of the reports which show increased presence of ß-catenin in severe and moderate dysplasia compared to mild dysplasia; however the expression of nuclear ß-catenin decreased after starting the invasive neoplastic process. This suggests a role for this protein in the progression of dysplasia and early malignant transformation to OSCC. Immunodetection of ß-catenin could be a possible immune marker in the detection of oral dysplasia.

Carcinoma de células escamosas intraóseo primario derivado de un tumor odontogénico queratoquístico: reporte de un caso y revisión de la literatura / Primary intraosseous squamous cell carcinoma derived from a keratocystic odontogenic tumour: Report of a case and a review of the literature

El carcinoma de células escamosas intraóseo primario (CCEIP) derivado de un tumor odontogénico queratoquístico (TOQ) es un tumor odontogénico maligno que se presenta con poca frecuencia y exclusivamente en los huesos maxilares. Afecta a personas de mediana edad, principalmente hombres y usualmente se localiza en la zona posterior mandibular. Clínicamente puede presentar las características clásicas de un tumor odontogénico benigno, aunque también puede asociarse a sintomatología dolorosa y alteraciones de la sensibilidad. Presentamos el caso de una mujer de 86 an˜ os de edad en la que se diagnosticó CCEIP derivado de un TOQ. Se describen las características clínicas, radiológicas e histológicas, discutiendo la importanciade tomar biopsia de distintas zonas de una lesión quística caracterizada por afectar amplias zonas de los huesos maxilares (AU)

Primary intraosseous squamous cell carcinoma (PIOSCC) derived from a keratocystic odontogenic tumour (KCOT) is a rare malignant bone tumour that exclusively involves the maxillary bones. It affects middle-age patients, mainly men, and is usually located in the posterior mandibular area. Clinically, it may exhibit classic characteristics of benign odontogenic tumors, though it may also be associated with pain and sensitive alterations We present the case of an 86-year-old woman who was diagnosed with a PIOSCC derived from a KCOT. The imaging, clinical, and histological characteristics are described, and the importance of taking a biopsy from different parts of a cystic lesion that is characterized by extensive anatomical area involved are discussed (AU)

Distribución del componente secretor en glándulas salivales de alcohólicos / Secretory component distribution in salivary glands of alcoholic subjects

En los pacientes alcohólicos se han reportado, además de una serie de alteraciones sistémicas, una pobre salud bucal, la que según algunos autores no se debería exclusivamente a una menor higiene bucal. Para probar la hipótesis de que la menor salud bucal de los pacientes alcohólicos se debe a alteraciones de la calidad de su saliva, se escogió el componente secretor como marcador de cambios en la secreción de las glándulas salivales de estos pacientes. Se estudiaron ocho muestras de glándulas salivales menores de labio de pacientes alcohólicos cirróticos, ocho muestras de alcohólicos no cirróticos y cuatro muestras controles. A estas biopsias se les aplicó una técnica inmunohistoquímica incubándolas con anticuerpo policlonal anti componente secretor. No se encontraron diferencias significativas en la ubicación del componente secretor al comparar los pacientes alcohólicos con los controles. Se corroboró la ubicación del componente secretor fundamentalmente en las semilunas de Gianuzzi y en conductos estriados, observándose igualmente en los bordes laterales de las células mucosas de los acinos