Efectos del extracto de alcoholes purificados de la cera de abejas (Apis mellifera) en dos modelos de analgesia / Effect of purified alcohols extract from the bee wax (Apis mellifera) in two analgesia models

El D-002 es una mezcla de 6 alcoholes alifàticos de alto peso molecular purificada de la cera de abejas (Apis mellifera). Este estudio tuvo como objetivo investigar los efectos del tratamiento por vía oral con D-002 sobre las contorsiones abdominales inducidas por àcido acético y en el modelo del plato caliente en ratones. Los animales se distribuyeron aleatoriamente en 5 grupos (10-20 animales/grupo): uno control que recibió el vehículo goma acacia/H2O, tres tratados con D-002 (25, 125 y 250 mg/kg) y uno con aspirina (modelo de contorsiones abdominales) o morfina (plato caliente). El D-002 (25-250 mg/kg) inhibió significativamente las contorsiones inducidas por àido acético en un 44,5; 44,8 y 47,1 por ciento respectivamente; sin embargo, no modificó la latencia de la respuesta en el modelo del plato caliente. Estos resultados muestran que el tratamiento por vía oral con D-002 (25-250 mg/kg) es capaz de inhibir de forma moderada las contorsiones abdominales por àcido acético sin afectar la respuesta al plato caliente. Esto sugiere que el D-002 ejerce una acción analgésica periférica pero no a nivel central.

The D-002 is a mixture of 6 high molecular weight aliphatic acids purified from bee wax (Apis mellifera). The aim of present study was to research the effects of an oral treatment using D-002 on the acetic acid- induced abdominal writhings and in the hot plate model in mice. Animals were randomized distributed to 5 groups (10-20 animals/group): a control one received the Gum Arabic vehicle/H(2)0, three received D-002 (25, 125 and 250 mg/kg), and another received aspirin (abdominal contortions model) or morphine (hot plate). The D-002 inhibited the above mentioned writhings in a 44,5; 44,8 and 47,1 respectively; however, not modified the response latency in the hot plate model. These results demonstrate that the D-002 (25-250 mg/kg) oral treatment may to inhibit in a moderate way the above mentioned writhings without to affect the response to hot plate. It suggests that the D-002 exerts a peripheral analgesic action but not a central level.

Efectos del extracto de alcoholes purificados de la cera de abejas (Apis mellifera) en dos modelos de analgesia / Efectos del extracto de alcoholes purificados de la cera de abejas (Apis mellifera) en dos modelos de analgesia / Effect of purified alcohols extract from the bee wax (Apis mellifera) in two analgesia models / Effect of purified alcohols extract from the bee wax (Apis mellifera) in two analgesia models

El D-002 es una mezcla de 6 alcoholes alifàticos de alto peso molecular purificada de la cera de abejas (Apis mellifera). Este estudio tuvo como objetivo investigar los efectos del tratamiento por vía oral con D-002 sobre las contorsiones abdominales inducidas por àcido acético y en el modelo del plato caliente en ratones. Los animales se distribuyeron aleatoriamente en 5 grupos (10-20 animales/grupo): uno control que recibió el vehículo goma acacia/H2O, tres tratados con D-002 (25, 125 y 250 mg/kg) y uno con aspirina (modelo de contorsiones abdominales) o morfina (plato caliente). El D-002 (25-250 mg/kg) inhibió significativamente las contorsiones inducidas por àido acético en un 44,5; 44,8 y 47,1 por ciento respectivamente; sin embargo, no modificó la latencia de la respuesta en el modelo del plato caliente. Estos resultados muestran que el tratamiento por vía oral con D-002 (25-250 mg/kg) es capaz de inhibir de forma moderada las contorsiones abdominales por àcido acético sin afectar la respuesta al plato caliente. Esto sugiere que el D-002 ejerce una acción analgésica periférica pero no a nivel central(AU)

El D-002 es una mezcla de 6 alcoholes alifàticos de alto peso molecular purificada de la cera de abejas (Apis mellifera). Este estudio tuvo como objetivo investigar los efectos del tratamiento por vía oral con D-002 sobre las contorsiones abdominales inducidas por àcido acético y en el modelo del plato caliente en ratones. Los animales se distribuyeron aleatoriamente en 5 grupos (10-20 animales/grupo): uno control que recibió el vehículo goma acacia/H2O, tres tratados con D-002 (25, 125 y 250 mg/kg) y uno con aspirina (modelo de contorsiones abdominales) o morfina (plato caliente). El D-002 (25-250 mg/kg) inhibió significativamente las contorsiones inducidas por àido acético en un 44,5; 44,8 y 47,1 por ciento respectivamente; sin embargo, no modificó la latencia de la respuesta en el modelo del plato caliente. Estos resultados muestran que el tratamiento por vía oral con D-002 (25-250 mg/kg) es capaz de inhibir de forma moderada las contorsiones abdominales por àcido acético sin afectar la respuesta al plato caliente. Esto sugiere que el D-002 ejerce una acción analgésica periférica pero no a nivel central(AU)

The D-002 is a mixture of 6 high molecular weight aliphatic acids purified from bee wax (Apis mellifera). The aim of present study was to research the effects of an oral treatment using D-002 on the acetic acid- induced abdominal writhings and in the hot plate model in mice. Animals were randomized distributed to 5 groups (10-20 animals/group): a control one received the Gum Arabic vehicle/H(2)0, three received D-002 (25, 125 and 250 mg/kg), and another received aspirin (abdominal contortions model) or morphine (hot plate). The D-002 inhibited the above mentioned writhings in a 44,5; 44,8 and 47,1 respectively; however, not modified the response latency in the hot plate model. These results demonstrate that the D-002 (25-250 mg/kg) oral treatment may to inhibit in a moderate way the above mentioned writhings without to affect the response to hot plate. It suggests that the D-002 exerts a peripheral analgesic action but not a central level(AU)

The D-002 is a mixture of 6 high molecular weight aliphatic acids purified from bee wax (Apis mellifera). The aim of present study was to research the effects of an oral treatment using D-002 on the acetic acid- induced abdominal writhings and in the hot plate model in mice. Animals were randomized distributed to 5 groups (10-20 animals/group): a control one received the Gum Arabic vehicle/H(2)0, three received D-002 (25, 125 and 250 mg/kg), and another received aspirin (abdominal contortions model) or morphine (hot plate). The D-002 inhibited the above mentioned writhings in a 44,5; 44,8 and 47,1 respectively; however, not modified the response latency in the hot plate model. These results demonstrate that the D-002 (25-250 mg/kg) oral treatment may to inhibit in a moderate way the above mentioned writhings without to affect the response to hot plate. It suggests that the D-002 exerts a peripheral analgesic action but not a central level(AU)

Acupuncture and rehabilitation of the painful shoulder: study protocol of an ongoing multicentre randomised controlled clinical trial [ISRCTN28687220].

BACKGROUND: Although the painful shoulder is one of the most common dysfunctions of the locomotor apparatus, and is frequently treated both at primary healthcare centres and by specialists, little evidence has been reported to support or refute the effectiveness of the treatments most commonly applied. According to the bibliography reviewed, physiotherapy, which is the most common action taken to alleviate this problem, has not yet been proven to be effective, because of the small size of sample groups and the lack of methodological rigor in the papers published on the subject. No reviews have been made to assess the effectiveness of acupuncture in treating this complaint, but in recent years controlled randomised studies have been made and these demonstrate an increasing use of acupuncture to treat pathologies of the soft tissues of the shoulder. In this study, we seek to evaluate the effectiveness of physiotherapy applied jointly with acupuncture, compared with physiotherapy applied with a TENS-placebo, in the treatment of painful shoulder caused by subacromial syndrome (rotator cuff tendinitis and subacromial bursitis). METHODS/DESIGN: Randomised controlled multicentre study with blind evaluation by an independent observer and blind, independent analysis. A study will be made of 465 patients referred to the rehabilitation services at participating healthcare centres, belonging to the regional public health systems of Andalusia and Murcia, these patients presenting symptoms of painful shoulder and a diagnosis of subacromial syndrome (rotator cuff tendinitis and subacromial bursitis). The patients will be randomised into two groups: 1) experimental (acupuncture + physiotherapy); 2) control (TENS-placebo + physiotherapy); the administration of rescue medication will also be allowed. The treatment period will have a duration of three weeks. The main result variable will be the change produced on Constant's Shoulder Function Assessment (SFA) Scale; as secondary variables, we will record the changes in diurnal pain intensity on a visual analogue scale (VAS), nocturnal pain intensity on the VAS, doses of non-steroid anti-inflammatory drugs (NSAIDs) taken during the study period, credibility scale for the treatment, degree of improvement perceived by the patient and degree of improvement perceived by the evaluator. A follow up examination will be made at 3, 6 and 12 months after the study period has ended. Two types of population will be considered for analysis: per protocol and per intention to treat. DISCUSSION: The discussion will take into account the limitations of the study, together with considerations such as the choice of a simple, safe method to treat this shoulder complaint, the choice of the control group, and the blinding of the patients, evaluators and those responsible for carrying out the final analysis.