RESUMO
The neuroprotective action by water-soluble form of quercetin was examined in gerbils after transient forebrain ischemia. The animals were exposed to 7 min of bilateral common carotid artery occlusion. Hippocampal CA 1 area was examined 7 days after ischemia-reperfusion. The average density of CA1 pyramidal neurons and GFAP-positive glial cells were counted in sham operated group, in ischemic group and in the groups treated with water-soluble form of quercetin. It was shown that quercetin revealed protective effect by decreasing of delayed neuronal death and reducing reactive astrogliosis after ischemia-reperfusion.
Assuntos
Isquemia Encefálica/tratamento farmacológico , Neurônios/efeitos dos fármacos , Fármacos Neuroprotetores/uso terapêutico , Quercetina/uso terapêutico , Animais , Astrócitos/efeitos dos fármacos , Astrócitos/metabolismo , Astrócitos/patologia , Isquemia Encefálica/metabolismo , Isquemia Encefálica/patologia , Isquemia Encefálica/prevenção & controle , Modelos Animais de Doenças , Gerbillinae , Proteína Glial Fibrilar Ácida/metabolismo , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Hipocampo/patologia , Imuno-Histoquímica , Masculino , Neurônios/metabolismo , Neurônios/patologia , Fármacos Neuroprotetores/administração & dosagem , Células Piramidais/efeitos dos fármacos , Células Piramidais/metabolismo , Células Piramidais/patologia , Quercetina/administração & dosagemRESUMO
The dynamic of structural and ultrastructural changes of CA1 area of the hippocampus was examined in rats after 10 or 15 min of global ischemia (4 vessels occlusion, 4VO) followed by reperfusion. In the early period of reperfusion (15 min, 2 h), the structural changes in synaptic terminals were observed, without any significant signs of neuronal damage. These changes consisted in (i) the increase of the relative number of perforated and multiple synapses, and (ii) the synaptic vesicles rearrangement. Clear neuronal damage appeared morphologically at 24 h, and then developed for 3-6 days and resulted in the delayed damage and death of the hippocampal neurons.
Assuntos
Comportamento Animal/fisiologia , Isquemia Encefálica/patologia , Hipocampo/ultraestrutura , Neurônios/ultraestrutura , Animais , Isquemia Encefálica/fisiopatologia , Modelos Animais de Doenças , Masculino , Microscopia Confocal , Microscopia Eletrônica , Ratos , Ratos WistarRESUMO
In the paper various methods for isolation and cultivation of endothelial cells are compared on the basis of the own data. The comparative analysis of the methods for isolation of endothelial cells from the vessel explant and by enzymatic treatment of aorta or coronary vessels is carried out. The methodical approaches to obtaining pure culture of endotheliocytes are being analysed.
