Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Sci Rep ; 8(1): 12770, 2018 08 24.
Artigo em Inglês | MEDLINE | ID: mdl-30143746

RESUMO

Bar-HRM is a hybrid method which combines DNA barcoding and High Resolution Melting analysis. It has proven to be a fast, cost-effective and reliable molecular approach for species identification and authentication. Here, three aspects of the use of Bar-HRM are focused on. First, Bar-HRM is used to discriminate between closely related Ophiocordyceps species. Second, identification of an unknown powder that is claimed to be Ophiocordyceps species using Bar-HRM. Third, authenticating the O. sinensis products sold on the market by the Bar-HRM. Results from HRM analyses with ITS primers shows that the two Ophiocordyceps species (Ophiocordyceps sinensis and Ophiocordyceps militaris) were easily differentiated. Also, an unknown sample was able to be identified in less time compared with using DNA barcoding alone. In addition, the substitution or adulteration of O. sinensis products sold on market was detected via Bar-HRM. The substitution or adulteration of inferior Ophiocordyceps species, particularly O. militaris in high price O. sinensis products has been a concern throughout Asia. Based on our results, the Bar-HRM was again proved to be a promising tool for species identification and authentication.


Assuntos
Código de Barras de DNA Taxonômico/métodos , Hypocreales/classificação , Hypocreales/genética , Desnaturação de Ácido Nucleico , Especificidade da Espécie , Temperatura de Transição
2.
Nucleosides Nucleotides Nucleic Acids ; 36(12): 726-735, 2017 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-29215948

RESUMO

DNA barcoding coupled high resolution melting (Bar-HRM) is an emerging method for species discrimination based on DNA dissociation kinetics. The aim of this work was to evaluate the suitability of different primer sets, derived from selected DNA regions, for Bar-HRM analysis of species in Kaempferia (Zingiberaceae). Four primer pairs were evaluated (rbcL, rpoC, trnL and ITS1). It was observed that the ITS1 barcode was the most useful DNA barcoding region overall for species discrimination out of all of the regions and primers assessed. Thus, the primer pair derived from the ITS1 region was the single most effective region for the identification of the tested species, whereas the rbcL primer pair gave the lowest resolution. Our Bar-HRM developed here would not only be useful for identification of Kaempferia plant specimens lacking essential parts for morphological identification but will be useful for authenticating products in powdered form of a high value medicinal species Kaempferia parviflora, in particular.


Assuntos
Código de Barras de DNA Taxonômico/métodos , DNA de Plantas/química , DNA de Plantas/genética , Zingiberaceae/classificação , Zingiberaceae/genética , Primers do DNA/genética , Mineração de Dados , Desnaturação de Ácido Nucleico , Plantas Medicinais/classificação , Plantas Medicinais/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...