Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Nat Commun ; 7: 12091, 2016 07 07.
Artigo em Inglês | MEDLINE | ID: mdl-27384705

RESUMO

Epigenetic mechanisms such as DNA methylation are essential regulators of the function and information storage capacity of neurons. DNA methylation is highly dynamic in the developing and adult brain, and is actively regulated by neuronal activity and behavioural experiences. However, it is presently unclear how methylation status at individual genes is targeted for modification. Here, we report that extra-coding RNAs (ecRNAs) interact with DNA methyltransferases and regulate neuronal DNA methylation. Expression of ecRNA species is associated with gene promoter hypomethylation, is altered by neuronal activity, and is overrepresented at genes involved in neuronal function. Knockdown of the Fos ecRNA locus results in gene hypermethylation and mRNA silencing, and hippocampal expression of Fos ecRNA is required for long-term fear memory formation in rats. These results suggest that ecRNAs are fundamental regulators of DNA methylation patterns in neuronal systems, and reveal a promising avenue for therapeutic targeting in neuropsychiatric disease states.


Assuntos
Região CA1 Hipocampal/metabolismo , Metilação de DNA , Epigênese Genética , Neurônios/metabolismo , Proteínas Oncogênicas v-fos/genética , RNA Mensageiro/genética , Animais , Região CA1 Hipocampal/citologia , Córtex Cerebral/citologia , Córtex Cerebral/metabolismo , Ilhas de CpG , Medo/fisiologia , Humanos , Injeções Intraventriculares , Masculino , Neurônios/citologia , Oligonucleotídeos Antissenso/administração & dosagem , Oligonucleotídeos Antissenso/genética , Oligonucleotídeos Antissenso/metabolismo , Proteínas Oncogênicas v-fos/antagonistas & inibidores , Proteínas Oncogênicas v-fos/metabolismo , Cultura Primária de Células , Regiões Promotoras Genéticas , RNA Mensageiro/metabolismo , Ratos , Ratos Sprague-Dawley , Técnicas Estereotáxicas
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...