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1.
Vet Clin Pathol ; 52(4): 554-568, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37254038

RESUMO

BACKGROUND: Enumeration of nucleated red blood cells (NRBCs) in peripheral blood of dogs and cats is performed by manual counting during blood film evaluation. Automated methods have increased precision and accuracy; however, most analyzers cannot distinguish leukocytes and NRBCs. The Sysmex XN-V Series may distinguish NRBCs and leukocytes; however, analytical errors occur. OBJECTIVES: We aimed to investigate cases with discrepant automated and manual NRBC counts, and to evaluate reasons for analytical errors. METHODS: Data from samples with increased NRBCs were collected retrospectively and compared with manual counts performed on blood films using Spearman's correlation, Passing-Bablok agreement analysis, and Bland-Altman comparisons. Precision of the automated method and interobserver agreement of manual counts were evaluated. Cases with discrepant results were investigated. RESULTS: Agreement between the methods was good at ≤1NRBC ×109 /L in dogs and cats, and inadequate at ≥1NRBC ×109 /L. The automated method demonstrated a negative proportional difference to the manual method. Precision was good for the automated method (overall CV 7.1%) and interobserver agreement for the manual method was poor overall (mean CV 27.3%, range 0%-106.1%). Inaccuracies in NRBC enumeration by the automated method occurred with high hematocrits, the mergence of the cell fragments and leukocyte clouds, and the presence of earlier erythroid precursors. CONCLUSIONS: NRBC enumeration by the WNR channel on the Sysmex XN-1000 V is precise and has good agreement with manual counts in canine and feline blood samples at ≤1NRBC ×109 /L. Manual film review is indicated for samples with ≥1NRBC ×109 /L, earlier erythroid precursors, samples from greyhounds and dehydrated patients, and if gating errors are noted.


Assuntos
Doenças do Gato , Doenças do Cão , Humanos , Animais , Gatos , Cães , Estudos Retrospectivos , Reprodutibilidade dos Testes , Eritroblastos
2.
Vet Clin Pathol ; 50(2): 227-235, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33913178

RESUMO

This is the first reported case of hypoglobulinemia in a dog with disseminated plasma cell neoplasia. A 6-year-old male intact Rottweiler was referred to the U-Vet Animal Hospital (Werribee, Vic, Australia) for weight loss, hyporexia, lethargy, vomiting, and soft stools. Examination of a buffy coat preparation and splenic and liver aspirates revealed a monomorphic population of plasmacytoid cells, and the same cells comprised approximately 90% of bone marrow samples submitted for cytologic and histologic evaluation. Biochemistry revealed a hypoglobulinemia, and the presence of an M-protein was not supported by serum and urine protein electrophoresis or serum immunofixation. Immunohistochemistry demonstrated strong nuclear labeling for MUM-1.


Assuntos
Doenças do Cão , Neoplasias de Plasmócitos , Plasmocitoma , Animais , Austrália , Doenças do Cão/diagnóstico , Cães , Masculino , Neoplasias de Plasmócitos/veterinária , Plasmócitos , Plasmocitoma/diagnóstico , Plasmocitoma/veterinária
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