RESUMO
Multilamellar and unilamellar phospholipid liposomes were prepared and investigated as regards their properties and the capacity of encapsulating biologically active materials, such as CrO4-(2-)ions, basic dyes, proteins, DNA, as well as Sendai virus particles. The efficiency of encapsulation ranged from 10% for CrO4-(2-)ions to about 80% for toluidine blue; it was found to depend not only on the type of encapsulated material, but also on the method used for liposome preparation and on liposome composition.
Assuntos
Lipossomos/farmacologia , Animais , Bovinos , Cromatos/farmacologia , Cromossomos Bacterianos , DNA Bacteriano/farmacologia , Interações Medicamentosas , Escherichia coli , Humanos , Lipossomos/isolamento & purificação , Métodos , Peso Molecular , Vírus da Parainfluenza 1 Humana , Proflavina/farmacologia , Albumina Sérica/farmacologia , Soroalbumina Bovina/farmacologia , Relação Estrutura-Atividade , Tiroxina/farmacologia , Cloreto de Tolônio/farmacologiaRESUMO
Anthracycline antibiotics--violamycin B1 and adriamycin--have an obvious effect on the efficiency of phage lambda L47.1 DNA transfection into E. coli Q358 cells. Treatment with anthracyclines of either phage DNA or bacterial cells results in a marked decrease in the number of transfectants per microgram DNA. On the other hand, adsorption of phage lambda gt WES to E. coli LE392 is considerably modified by exposure to anthracyclines of either the phage or the host cells.
Assuntos
Antibacterianos/farmacologia , Escherichia coli/efeitos dos fármacos , Etídio/farmacologia , Adsorção , Aminoglicosídeos/farmacologia , Antibióticos Antineoplásicos , DNA Viral/genética , Relação Dose-Resposta a Droga , Doxorrubicina/farmacologia , Escherichia coli/genética , Cinética , Naftacenos/farmacologia , Transfecção/efeitos dos fármacosRESUMO
Direct examination of urethral smears made evident the presence of chlamydial infection in 162 (38.1%) of 425 male patients with nongonococcal urethritis. Serum antibodies to the group antigen could be detected by complement fixation and microagglutination reactions only in about 50% of the cases with chlamydial infection. The proportion of chlamydial strains isolated in chick embryos was rather low (14.8%) and the isolation rate appeared to correlate with the abundance of urethral secretion.
Assuntos
Linfogranuloma Venéreo/epidemiologia , Uretrite/etiologia , Testes de Aglutinação , Anticorpos Antibacterianos/análise , Chlamydia trachomatis/imunologia , Chlamydia trachomatis/isolamento & purificação , Testes de Fixação de Complemento , Humanos , Linfogranuloma Venéreo/diagnóstico , Masculino , Uretra/metabolismo , Uretrite/diagnósticoRESUMO
Seroconversion to different viral, chlamydial, rickettsial and mycoplasma antigens was followed up in 134 children aged 0-6 years, hospitalized with different respiratory diseases. Parainfluenza viruses type 1, 2 and 3 and adenoviruses appeared to be involved in the etiology of most of the cases; respiratory syncytial virus was often found to play a role in pneumonia/bronchopneumonia and in "influenza-like illness", while chlamydiae and M. pneumoniae could be incriminated in cases of "influenza-like illness", as well as in the other categories of respiratory disease. Mixed infections with the agents studied could be detected.
