Assuntos
Bases de Dados Factuais , Sistemas Computadorizados de Registros Médicos , Garantia da Qualidade dos Cuidados de Saúde , Traumatismos da Medula Espinal , Sistemas de Apoio a Decisões Clínicas , Dinamarca , Humanos , Admissão do Paciente , Planejamento de Assistência ao Paciente , Equipe de Assistência ao Paciente , Alta do Paciente , Modalidades de Fisioterapia , Apoio Social , Traumatismos da Medula Espinal/diagnóstico , Traumatismos da Medula Espinal/fisiopatologia , Traumatismos da Medula Espinal/reabilitação , Traumatismos da Medula Espinal/terapiaRESUMO
An iron-sulfur metalloprotein containing the 5-12 and 35-50 residues of Desulfovibrio gigas rubredoxin has been synthesized by Fmoc solid phase peptide synthesis and subsequent peptide folding. A Gly links the two residue chains between Val-5 and Glu-50. Sybyl Tripos structure optimization indicates only minor structural changes of the folded synthetic protein compared to the similar residue positions in the native protein. The UV-VIS spectrum of the reduced synthetic protein is very similar to that of native D. gigas rubredoxin and the molecular mass determined by laser mass spectrometry has the expected value (+/- 2D). No metal is transferred to the gas phase by the laser beam merely by mixing the peptide and iron(II), substantiating that the folding procedure is a necessary pre-requisite for protein formation. The Val-->Leu41 chemical mutant has also been synthesized and behaves in a closely similar fashion.
Assuntos
Mutação , Rubredoxinas/biossíntese , Sequência de Aminoácidos , Leucina/genética , Dados de Sequência Molecular , Rubredoxinas/química , Rubredoxinas/metabolismo , Análise Espectral , Valina/genéticaRESUMO
Growing skull fractures with development of leptomeningeal cysts are rare complications of head injuries and have not been described in the perinatal period. The case history of a newborn with bilateral parietal fractures and the formation of a leptomeningeal cyst on one side detected at birth is presented. The importance of radiographic evaluation, including skull films, computered tomography, and magnetic resonance imaging, as well as the associated subarachnoid cyst and the age of presentation are discussed.
