Core carbon fixation pathways associated with cake layer development in an anoxic-oxic biofilm-membrane bioreactor treating textile wastewater.

Microbial carbon fixation pathways have not yet been adequately understood for their role in membrane case layer formation processes. Carbon fixation bacteria can play critical roles in either causing or enhancing cake layer formation in some autotrophic-prone anoxic conditions, such as sulfur-cycling conditions. Understanding the microbes capable of carbon fixation can potentially guide the design of membrane biofouling mitigation strategies in scientific ways. Thus, we used meta-omics methods to query carbon fixation pathways in the cake layers of a full-scale anoxic-oxic biofilm-MBR system treating textile wastewater in this study. Based on the wastewater constituents and other properties, such as anoxic conditions, sulfide-reducing and sulfur-oxidizing bacteria could co-exist in the membrane unit. In addition, low-light radiation conditions could also happen to the membrane unit. However, we could not quantify the light intensity or total energy input accurately because the whole experimental setup was a full-scale system. Potentially complete carbon fixation pathways in the cake layer included the Calvin-Benson-Bassham cycle, Wood-Ljungdahl pathway, and the 3-hydroxypropionate bicycle. We discovered that using aeration could effectively inhibit carbon fixation, which resulted in mitigating membrane cake layer development. However, the aeration resulted in the 3-hydroxypropionate bicycle pathway, presumably used by aerobic sulfur-oxidizing prokaryotes, to become a more abundant carbon fixation pathway in the cake layer under aerobic conditions.

Dissimilatory sulfate reduction in the cake layer of a full-scale anaerobic dynamic membrane bioreactor for hotel laundry wastewater treatment: Bacterial community and functional genes.

Dissimilatory sulfate reduction (DSR) in cake layer of full-scale anaerobic dynamic membrane bioreactor for treating hotel laundry wastewater was studied. Change (Δ) of sulfate concentration (ΔSO42-) was positively correlated to dynamic cake layer (DCL) development, while ΔS2- was negatively correlated. ΔSO32- and ΔSorganic sulfur remained around 1.5-2.5 and 1.2-2.3 mg-S/L, respectively. Thus, DSR was the predominant sulfate reduction process in DCL. 33 binned genomes from DCL microbiome samples possessed one or more DSR functional genes. But only four binned genomes possess all functional genes, and thus can achieve complete DSR. However, no significant variations of these DSR bacteria was obseared during DCL development. Metagenomic analysis predicted that sulfate reduction in DCL was mainly carried out by collaborations between bacteria with incomplete DSR pathways. Among which, sulfite â†’ sulfide by dissimilatory-sulfite-reductase expression bacteria was the key process. Overall results suggested that controlling dissimilatory-sulfite-reductase activities could prevent sulfide buildup in the effluent.

Assimilatory and dissimilatory sulfate reduction in the bacterial diversity of biofoulant from a full-scale biofilm-membrane bioreactor for textile wastewater treatment.

Assimilatory and dissimilatory sulfate reduction (ASR and DSR) are the core bacterial sulfate-reducing pathways involved in wastewater treatment. It has been reported that sulfate-reducing activities could happen within biofoulants of membrane bioreactors during wastewater treatment. Biofoulants are mainly microbial products contributing membrane fouling and subsequent rising energy consumption in driving membrane filtration. Biofoulants from a full-scale biofilm-membrane bioreactor (biofilm-MBR) treating textile wastewater were investigated in this study. During a 10-month operation, sulfate concentrations in the effluent of the biofilm-MBR gradually decreased alongside with the creeping up sulfite concentrations when biofoulants were also building up on membrane modules. Sulfide had no apparent increases in the effluent during this period. Metagenomic analysis revealed diverse microbial communities residing in the biofoulants. Further analysis on their genetic traits revealed abundant ASR's and DSR's functional genes. A plethora of sulfate-reduction bacteria (SRB), including the well-known Desulfovibrio, Desulfainum, Desulfobacca, Desulfobulbus, Desulfococcus, Desulfonema, Desulfosarcina, Desulfobacter, Desulfobacula, Desulfofaba, Desulfotigum, Desulfatibacillum, Desulfatitalea, Desulfobacterium, were detected in the biofoulants. They were believed to play some important carbon and sulfur-cycling roles in our study. Based on metagenomic analysis, we also deduced that ASR was a functionally more important sulfate-reducing route because of the high abundance of assimilatory sulfate reductases detected. Also, the "AMP (adenosine monophosphate)→sulfite" step was a key reaction shared by both ASR and DSR in the biofoulant. This step might be responsible for the sulfite accumulation in the biofilm-MBR effluent. Overall, ASR functional genes in the biofoulants were more abundant. But the bacteria possessing complete DSR pathways caused the sulfide production in the biofilm-MBR.

Core nitrogen cycle of biofoulant in full-scale anoxic & oxic biofilm-membrane bioreactors treating textile wastewater.

Core nitrogen cycle within biofoulant in full-scale anoxic & oxic biofilm-membrane bioreactor (bMBR) treating textile wastewater was investigated. Wastewater filtered through membrane with biofoulant had elevated NH4+-N and NO2--N concentrations corresponding to decreased NO3--N concentrations. Nevertheless, total nitrogen concentrations did not change significantly, indicating negligible nitrogen removal activities within biofoulant. Metagenomic analysis revealed a lack of genes, such as AmoCAB and Hao in biofoulant, indicating absence of nitrification or anammox populations. However, genes encoding complete pathway for dissimilatory nitrate reduction to ammonium (DNRA) were discovered in 15 species that also carry genes encoding both nitrate reductase and nitrite reductase. No specie contained all genes for complete denitrification pathway. High temperature, high C:N ratio, and anoxic conditions of textile wastewater could favorite microbes growth with DNRA pathway over those with canonical denitrification pathway. High dissolved oxygen concentrations could effectively inhibit DNRA to minimize ammonia concentration in the effluent.