Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Tuberculosis (Edinb) ; 107: 73-79, 2017 12.
Artigo em Inglês | MEDLINE | ID: mdl-29050775

RESUMO

Tuberculosis (TB) remains a major worldwide health problem and has caused millions of deaths in the past few years. Current diagnostic methods, such as sputum smear microscopy and sputum culture, are time-consuming and cannot prevent the rapid spreading of TB during the diagnostic period. In this connection, detecting biomarkers specific to TB at molecular level in plasma of patients will provide a rapid means for diagnosis. In this study, we first evaluated the differential expression of the long non-coding RNAs (lncRNAs) in the plasma from patients with TB (TB positive), community acquired pneumonia (CAP) and healthy individuals (CG) using lncRNA microarray scanning. It was found that there were 2116 specific lncRNAs differentially expressed in the TB positive samples (1102 up-regulated and 1014 down-regulated), which accounted for 6.96% of total lncRNAs. Twelve differentially expressed lncRNAs discovered in microarray were subsequently validated by using real-time quantitative PCR (RT-qPCR). Two lncRNAs (ENST00000354432 and ENST00000427151) were further validated with more Tuberculosis samples. These results suggested the expression level of lncRNAs and the two validated lncRNAs in plasma could be the potential molecular biomarkers for the rapid diagnosis of Tuberculosis.


Assuntos
Perfilação da Expressão Gênica/métodos , Mycobacterium tuberculosis/patogenicidade , Análise de Sequência com Séries de Oligonucleotídeos , RNA Longo não Codificante/genética , Tuberculose Pulmonar/diagnóstico , Estudos de Casos e Controles , Diagnóstico Diferencial , Marcadores Genéticos , Interações Hospedeiro-Patógeno , Humanos , Mycobacterium tuberculosis/isolamento & purificação , Valor Preditivo dos Testes , RNA Longo não Codificante/sangue , Reação em Cadeia da Polimerase em Tempo Real , Reprodutibilidade dos Testes , Escarro/microbiologia , Transcriptoma , Tuberculose Pulmonar/sangue , Tuberculose Pulmonar/microbiologia , Fluxo de Trabalho
2.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 30(6): 1326-9, 2013 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-24645620

RESUMO

The aim of this study was to build a gene chip system with surface plasmon resonance (SPR) technique, for which Gamma-peptide nucleic acid (Gamma-PNA) functioned as a probe, in order to improve sensitivity and its specificity. With the use of self-assembled monolayer (SAM) technology, surface chemistry of two-dimensional structure was used. Gamma-PNA was designed according to the bioinformatics, and was plated on the SPR chip modified by SAM. Subsequently, relevant parameters of the experiment were ensured and optimized. The results showed that the performances of Gamma-PNA probe was little affected by the ion concentration of buffer, and it had a strong light signal in a stable state. As the ion concentration was 0, there were still good hybrid reactions; pH value had less influence upon Gamma-PNA probe, and acid environment of buffer could be better. Gamma-PNA probe combined with sensor technologies achieved made the probe with dispensable labels and real-time detection. It also improved the efficiency of the hybridization and the stability, providing the foundation for clinical application.


Assuntos
Análise de Sequência com Séries de Oligonucleotídeos/métodos , Ácidos Nucleicos Peptídicos/genética , Ressonância de Plasmônio de Superfície , Hibridização de Ácido Nucleico , Sondas de Ácido Nucleico , Sensibilidade e Especificidade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...