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1.
Genetics ; 183(2): 483-96, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19667134

RESUMO

Homologous chromosomes are paired in somatic cells of Drosophila melanogaster. This pairing can lead to transvection, which is a process by which the proximity of homologous genes can lead to a change in gene expression. At the yellow gene, transvection is the basis for several examples of intragenic complementation involving the enhancers of one allele acting in trans on the promoter of a paired second allele. Using complementation as our assay, we explored the chromosomal requirements for pairing and transvection at yellow. Following a protocol established by Ed Lewis, we generated and characterized chromosomal rearrangements to define a region in cis to yellow that must remain intact for complementation to occur. Our data indicate that homolog pairing at yellow is efficient, as complementation was disrupted only in the presence of chromosomal rearrangements that break

Assuntos
Cromossomos/genética , Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Animais , Aberrações Cromossômicas , Feminino , Duplicação Gênica , Regulação da Expressão Gênica , Teste de Complementação Genética , Masculino , Modelos Genéticos , Mutação , Translocação Genética
2.
Proc Natl Acad Sci U S A ; 99(6): 3723-8, 2002 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-11904429

RESUMO

Interactions between paired homologous genes can lead to changes in gene expression. Such trans-regulatory effects exemplify transvection and are displayed by many genes in Drosophila, in which homologous chromosomes are paired somatically. Transvection involving the yellow cuticle pigmentation gene can occur by at least two mechanisms, one involving the trans-action of enhancers on a paired promoter and a second involving pairing-mediated bypass of a chromatin insulator. A system was developed to evaluate whether the action of the yellow enhancers in trans could be reconstituted outside of the natural near telomeric location of the yellow gene. To this end, transgenic flies were generated that carried a yellow gene modified by the inclusion of strategically placed recognition sites for the Cre and FLP recombinases. Independent action of the recombinases produced a pair of derivative alleles, one enhancerless and the other promoterless, at each transgene location. Transvection between the derivatives was assessed by the degree of interallelic complementation. Complementation was observed at all eight sites tested. These studies demonstrate that yellow transvection can occur at multiple genomic locations and indicate that the Drosophila genome generally is permissive to enhancer action in trans.


Assuntos
Drosophila melanogaster/genética , Elementos Facilitadores Genéticos/genética , Regulação da Expressão Gênica , Genes de Insetos/genética , Proteínas de Insetos/genética , Alelos , Animais , Animais Geneticamente Modificados , Sítios de Ligação Microbiológicos/genética , Proteínas de Drosophila/genética , Feminino , Teste de Complementação Genética , Engenharia Genética , Genoma , Células Germinativas/metabolismo , Integrases/genética , Integrases/metabolismo , Masculino , Fenótipo , Regiões Promotoras Genéticas/genética , Recombinação Genética/genética , Transgenes/genética , Proteínas Virais/genética , Proteínas Virais/metabolismo
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