Stigmas and Petals of Crocus sativus L. (Taliouine, Morocco): Comparative Evaluation of Their Phenolic Compounds, Antioxidant, and Antibacterial Activities.

The dried stigmas of Crocus sativus L. produce saffron, a precious spice used for its culinary and medicinal properties since ancient times, while its petals are considered the main by-product of saffron production. The present study aimed to comparatively evaluate the phenolic content, antioxidant capacity, and antibacterial activity of methanolic extracts of stigmas and petals of Crocus sativus L. from Taliouine. The polyphenol content was measured using the Folin-Ciocalteu method, the antioxidant activity was determined using the DPPH free radical scavenging method, and the well-diffusion method was used to assess antibacterial activity against seven pathogenic bacterial strains (Bacillus subtilis, Escherichia coli, Listeria monocytogenes, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella enterica, and Staphylococcus aureus). Furthermore, the minimum inhibitory concentration (MIC) of the extracts was determined using the microdilution broth test. Our findings revealed that stigmas and petals contained phenolic compounds at the rate of 56.11 ± 4.70 and 64.73 ± 3.42 mg GAE/g, as well as DPPH radical scavenging capacity with IC50 of 1700 µg/ml and 430 µg/ml, respectively. Petal extract showed more effective antibacterial activity, with inhibition diameters ranging from 10.66 ± 0.57 to 22.00 ± 1.00 mm and MIC values ranging from 2.81 to 5.62 mg/ml, compared to the stigma extract, which displayed inhibition diameters from 10.00 ± 0.00 to 18.67 ± 0.76 mm and MIC from 2.81 to 11.25 mg/ml, against five of the seven bacterial strains tested, including S. aureus, E. coli, P. vulgaris, P. aeruginosa, and S. enterica. Statistical analyses were performed to determine the significance of these results. Thus, stigmas and petals of Crocus sativus L. might serve as a suitable source of natural antioxidant and antimicrobial agents for application in the food and pharmaceutical industries.

Molecular docking of quercetin: a promising approach for the development of new anti-inflammatory and analgesic drugs.

The aim of this study is to investigate the antinociceptive, anti-inflammatory and antipyretic effects of quercetin. Additionally, molecular docking studies were conducted to evaluate potential interactions between quercetin and various molecular targets. Animal models were used to conduct a comprehensive pharmacological investigation of quercetin. Evaluation of analgesic activity revealed a reduction in the number of abdominal cramps during the twisting test and inhibition of pain during the second phase of the formaldehyde test. Additionally, evaluation of its anti-inflammatory activity showed a reduction in ear oedema. However, it is important to note that quercetin administration has not been shown to significantly reduce yeast-induced hyperthermia. The docking study revealed the high inhibitory potential of quercetin against the COX-2 receptor.

Influence of Drying Temperature on the Different Thermodynamic Parameters during the Indirect Convective Solar Drying of Crocus sativus L. Of Morocco Thin-Layer Solar Drying of Moroccan Saffron.

This work deals with the study of the drying kinetics of Crocus sativus L. using convective solar drying. The main objective was to identify the influence of airflow drying temperatures for ambient air temperature ranged between 15.6 and 18.9°C, and a relative humidity between 24.4 and 46.5%. The equilibrium moisture content varies from 0.09 to 0.06 (% d.b), respectively, for drying air temperatures 35-50°C. The airflow velocity was about 0.2 m s-1, which implied establishing a phenomenological diffusion model of the water within the matrix. Empirical models were also determined as well as a polynomial equation (order 3) of the characteristic drying curve. The Midilli-Kucuk model was found to be the best to describe the experimental drying curves of Crocus sativus L. The effective moisture diffusivity ranged between 0.87 and 1.46 10-11 m2 s-1 for airflow temperature 35 and 50°C, while the average activation energy was calculated as 28.76 kJ mol-1. The increase in temperature decreases the total energy consumption which varies, respectively, from 3.211 to 2.681 kWh.