RESUMO
BACKGROUND/AIMS: To investigate postulated differences related to FGF-2 in liver morphology and expression of apoptosis-related factors after partial hepatectomy (PH). METHODS: Homogenous FGF-2-deficient mice (C57BL/6J) with their FGF-2-(+/+) littermates (control) were used to examine the structure of regenerating livers after PH with light and electron microscopy. The regenerative response and BrDu incorporation were monitored. The expression of BclX-l, Bax, Fas, TNF-alpha, and Caspase-3 were measured by reverse transcription PCR (RT-PCR) and Northern blot analysis. RESULTS: In the FGF-2-(-/-) group, hepatocytes and endothelial cells contain mitochondria with atypical cristae and fragmented endoplasmic reticulum structures compared to control. Sinusoids show irregular basal laminae. These changes are in accordance with a differential expression of apoptosis-related factors: FasL was expressed throughout the entire observation span (days 0 to 10 post-PH). Following PH, tumor necrosis factor alpha (TNFalpha)-mRNA levels were higher in FGF-2-(+/+) animals, while Fas as well as Bax and BclXl were overexpressed in FGF-2-(-/-) mice. Caspase-3-mRNA was similarly expressed in both groups, but Caspase-3 activity was elevated for 4 days in FGF-2-(-/-) mice. CONCLUSION: Despite morphologic differences, differences in the time schedule of DNA synthesis and differences in apoptotic response, the dynamics of liver regeneration in FGF-2-(-/-) mice were not impaired.
Assuntos
Apoptose/fisiologia , Fator 2 de Crescimento de Fibroblastos/genética , Hepatócitos/ultraestrutura , Regeneração Hepática/fisiologia , Animais , Apoptose/genética , Caspase 3 , Caspases/análise , Caspases/genética , Caspases/metabolismo , Tamanho Celular , Retículo Endoplasmático/ultraestrutura , Proteína Ligante Fas , Fator 2 de Crescimento de Fibroblastos/fisiologia , Expressão Gênica , Hepatectomia , Hepatócitos/química , Hepatócitos/fisiologia , Fígado/ultraestrutura , Regeneração Hepática/genética , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Camundongos , Camundongos Mutantes , Mitocôndrias/ultraestrutura , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , RNA Mensageiro/análise , RNA Mensageiro/metabolismo , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo , Proteína X Associada a bcl-2 , Proteína bcl-X , Receptor fas/genética , Receptor fas/metabolismoRESUMO
OBJECTIVE: The study goal was to report on the long-term results and effect of argon plasma coagulation (APC) surgery and topical estriol in patients with Osler's disease who had recurrent epistaxis. Study design In a prospective clinical study, 52 patients underwent APC and estriol application and were followed for 18 months regarding their bleeding frequency and intensity. Patient blood samples were obtained to determine the serum estriol levels. Scanning electron microscopy of the nasal mucosa enabled a better understanding concerning the effect of estriol on the nasal mucosa. RESULTS: Eighteen months after treatment, 96% of the patients stated a significantly reduced bleeding frequency and intensity. Under estriol influence, former berry-like telangiectasia of the nasal mucosa was flatter. The serum estriol levels did not increase significantly in any of the patients. No side effects from the use of topical estriol were observed. CONCLUSION: The combined treatment approach with APC and topical estriol significantly reduces epistaxis in Osler's disease. SIGNIFICANCE: APC and topical estriol have proved to be a promising alternative in the treatment of Osler's disease.
