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1.
Arch Otolaryngol Head Neck Surg ; 125(8): 907-11, 1999 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10448739

RESUMO

Merkel cell carcinoma of the lower lip is a rare clinical entity. It is generally thought to be more aggressive than the more common skin cancers of the lips and oral cavity. The literature reveals that delayed diagnoses and poor survival are common among patients with these malignancies. Recommended treatment is surgical excision with wide margins and treatment of cervical metastases with either surgery or radiotherapy or both. Unlike other malignant neoplasms, the occurrence of Merkel cell carcinoma in a relatively older population makes comorbid conditions a key factor in treatment planning.


Assuntos
Carcinoma de Célula de Merkel/patologia , Lábio , Neoplasias Cutâneas/patologia , Idoso , Biópsia por Agulha , Carcinoma de Célula de Merkel/radioterapia , Carcinoma de Célula de Merkel/cirurgia , Feminino , Humanos , Metástase Linfática , Neoplasias Cutâneas/radioterapia , Neoplasias Cutâneas/cirurgia
2.
J Biol Chem ; 262(7): 3409-14, 1987 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-2950097

RESUMO

Dynein 1 was extracted from sperm flagella of the sea urchin Tripneustes gratilla with 0.6 M NaCl and dialyzed against 0.5 mM EDTA, 14 mM 2-mercaptoethanol, 5 mM imidazole/HCl buffer, pH 7.0, for 24-48 h. In some cases, fractions containing the alpha heavy chain and the beta/intermediate chain 1 complex (beta/IC1) were separated by density gradient centrifugation in the same solution. Treatment of the samples at a trypsin:protein ratio of 1:10 w/w for 32 min at room temperature yields a crude digest from which Fragment A is purified by density gradient centrifugation. The purified Fragment A consists of two principal peptides (Mr = 195,000 and 130,000) that cosediment with the peak of ATPase activity at 12.5 S, which is slightly faster than the 11 S of the original beta/IC1 complex. When digests of the separated alpha chain and of the beta/IC1 complex are followed as a function of time, the early cleavages of the two heavy chains (Mr = 428,000) resemble each other in that both lead to similarly sized peptides of Mr 316,000 and 296,000, but only in the beta/IC1 fraction does the digestion proceed to form Fragment A. The remainder of the beta chain, termed Fragment B, occurs as an Mr 110,000 peptide sedimenting at 5.7 S with no associated ATPase activity. Fragment A has a specific ATPase activity of 4.3 mumol Pi X min-1 X mg-1, with a Km of 29 microM in 0.1 M NaCl medium, and an apparent Ki for inhibition by vanadate of 1.2 microM in the absence of salt, and 22 microM in 0.6 M NaCl. Photoaffinity labeling with [alpha-32P]8-azidoadenosine 5'-triphosphate indicates that the ATP binding site on the beta chain of dynein 1 is located on the Mr 195,000 peptide of Fragment A. The possibility that Fragments A and B of the beta/IC1 complex may correspond to the head and tail regions of the tadpole-shaped particle seen by electron microscopy is discussed.


Assuntos
Adenosina Trifosfatases/metabolismo , Dineínas/metabolismo , Flagelos/análise , Fragmentos de Peptídeos/metabolismo , Ouriços-do-Mar/análise , Espermatozoides/análise , Tripsina/metabolismo , Adenosina Trifosfatases/antagonistas & inibidores , Trifosfato de Adenosina/análogos & derivados , Trifosfato de Adenosina/metabolismo , Animais , Azidas/metabolismo , Sítios de Ligação , Centrifugação com Gradiente de Concentração , Eletroforese em Gel de Poliacrilamida , Masculino , Peso Molecular , Fragmentos de Peptídeos/isolamento & purificação , Vanadatos , Vanádio/farmacologia
3.
J Biol Chem ; 261(5): 2337-42, 1986 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-2935534

RESUMO

Irradiation of soluble dynein 1 from sea urchin sperm flagella at 254 nm in the presence of 50 microM ATP and 100 microM inorganic vanadate (Vi) cleaves the alpha and beta heavy chains into approximately equal quantities of two polypeptides of Mr 228,000 and 200,000, with a conversion efficiency of about 63%. A similar cleavage occurs in the presence of Vi and either ADP or 8-azidoadenosine 5'-triphosphate (8-N3ATP); in the latter case, 8-N3ATP becomes covalently bound principally to the Mr 228,000 polypeptide. No detectable amount of these fragments is formed if either the Vi or the nucleotide is omitted or in the presence of Vi and 50 microM AMP. These results emphasize the basic similarity of the two ATPases associated with the alpha and beta heavy chain subunits of dynein 1 and give a mean Mr of 428,000 for the intact heavy chains.


Assuntos
Adenosina Trifosfatases/efeitos da radiação , Trifosfato de Adenosina/análogos & derivados , Trifosfato de Adenosina/metabolismo , Azidas/metabolismo , Dineínas/efeitos da radiação , Vanádio/metabolismo , Animais , Dineínas/metabolismo , Eletroforese em Gel de Poliacrilamida , Flagelos/análise , Masculino , Peso Molecular , Peptídeos/análise , Ouriços-do-Mar , Espermatozoides/análise , Raios Ultravioleta , Vanadatos
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