GSM 900 MHz Microwave Radiation-Induced Alterations of Insulin Level and Histopathological Changes of Liver and Pancreas in Rat.

BACKGROUND: The rapidly increasing use of mobile phones has led to public concerns about possible health effects of these popular communication devices. This study is an attempt to investigate the effects of radiofrequency (RF) radiation produced by GSM mobile phones on the insulin release in rats. METHODS: Forty two female adult Sprague Dawley rats were randomly divided into 4 groups. Group1 were exposed to RF radiation 6 hours per day for 7 days. Group 2 received sham exposure (6 hours per day for 7 days). Groups 3 and 4 received RF radiation 3 hours per day for 7 days and sham exposure (3 hours per day), respectively. The specific absorption rate (SAR) of RF was 2.0 W/kg. RESULTS: Our results showed that RF radiations emitted from mobile phone could not alter insulin release in rats. However, mild to severe inflammatory changes in the portal spaces of the liver of rats as well as damage in the cells of islet of Langerhans were observed. These changes were linked with the duration of the exposures. CONCLUSION: RF exposure can induce inflammatory changes in the liver as well causing damage in the cells of islet of Langerhans.

Renal inflammatory response to urinary tract infection in rat neonates.

Urinary tract infection (UTI) is one of the most common bacterial infections. Maternal UTI is a risk factor for neonatal UTI. The aim of the present study was to determine the severity of renal inflammation in neonate rats born from mothers with induced UTI. Twelve pregnant rats (Sprague-Dawley) were included in study. The rats were divided into two groups (six rats in each group). In the first group, pyelonephritis was induced in the third trimester of pregnancy and the second group was used as a control group. After delivery, the neonates were divided into three groups based on days after birth (the 1 st, 3 rd and 7 th days after birth). In each group, two neonates of each mother were killed and a midline abdominal incision was made and both kidneys were aseptically removed. On the 7 th day, rat mothers were killed and their kidneys were removed. The preparations were evaluated with a bright field microscope for inflammatory response. Renal pathology showed inflammation in all UTI-induced mothers, but only two cases of neonates (2.1%) showed inflammation in the renal parenchyma. There was no relation between the positive renal culture and the pathological changes. We conclude that neonates with UTI born to UTI-induced mothers showed a lesser inflammatory response.

Early renal post-ischaemic tissue damage and dysfunction with contribution of A1-adenosine receptor activation in rat.

AIM: This study investigated the effect of a selective A(1)-adenosine receptor (A(1)-AR) antagonist, 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), on the renal dysfunction and histological damage induced by ischaemia/reperfusion at an early stage. METHODS: Pentobarbital anaesthetised rats were prepared for measuring renal functional variables. Ischaemia was induced by bilateral renal artery clamping for 30 min followed by a 4 h reperfusion period. In DPCPX-treated rats, it was infused (i.v.) at 10 microg/kg per min before and after renal ischaemia. Both kidneys were examined using light and electron microscopy. RESULTS: The renal ischaemic challenge resulted in major histological and ultrastructural damages, which were associated with decreased creatinine clearance, absolute potassium-excretion and effective free-water reabsorption, but increased fractional sodium-excretion and urine flow during reperfusion period. In DPCPX-treated rats, the histological and ultrastructural damage to the kidneys was improved along with the decrease in creatinine clearance and increase in fractional sodium-excretion being smaller, but the increase in urine flow being larger than those of the non-treated rats, while absolute potassium-excretion and effective free-water reabsorption were equal to those of the sham-operated rats. CONCLUSION: These findings suggest that endogenous activation of A(1)-AR contributes to the early development of renal ischaemia/reperfusion injury.

Intraoperative fine needle aspiration diagnosis of cardiac rhabdomyoma. A case report.

BACKGROUND: Cardiac rhabdomyoma is a very common benign tumor in infants and children. It can be confused clinically with a malignant tumor. CASE: A 6-year-old girl was admitted with a chief complaint of breathlessness. Chest roentgenography revealed cardiomegaly. Echocardiography showed a right atrial mass. Intraoperative fine needle aspiration showed groups of large, polygonal cells with granular, vacuolated cytoplasm. Rarely were intranuclear inclusions observed. CONCLUSION: The cytologic findings were characteristic of rhabdomyoma.

Extramedullary plasmacytoma. Fine needle aspiration findings.

OBJECTIVE: To determine the role of fine needle aspiration cytology in the diagnosis of extramedullary plasmacytoma. STUDY DESIGN: The study group consisted of 13 patients with palpable masses at various sites. The tumors were aspirated for cytologic study. RESULTS: The smears revealed groups of mature and immature plasma cells at various stages of maturation. Mature plasma cells showed an eccentric nucleus and abundant, deep, basophilic cytoplasm with a paranuclear halo. Plasmablasts (immature plasma cells) showed a prominent, eccentric nucleus with single, large nucleolus and abundant, deep, basophilic cytoplasm with no paranuclear halo. Binucleate and multinucleate forms were also seen quite often. The tumors were excised, and the histologic sections confirmed the cytologic diagnosis. All the patients received radiotherapy. One patient (18 years old) developed recurrence and died due to extensive infiltration into the maxilla and mandible. Two patients (57 and 62 years) developed multiple myeloma one to two years after the excision of tumors, and both died two to three months later. The remaining 10 patients were alive and well at this writing. CONCLUSION: The smears from all 13 patients were diagnosed as extramedullary plasmacytomas by fine needle aspiration cytology.

Tuberculous orchitis diagnosed by fine needle aspiration cytology.

OBJECTIVE: To study the role of the fine needle aspiration cytology (FNAC) in the diagnosis of testicular lesions. STUDY DESIGN: Seven adult males (55-68 years) presented to the clinic with unilateral enlargement of the testis for the previous few months. Clinically, a malignant tumor was suspected. Fine needle aspiration was performed for rapid diagnosis. RESULTS: The smears revealed many groups of epithelioid histiocytes, giant cells, a few plasma cells and occasional spindle-shaped fibroblasts. Acid-fast stain revealed a few acid-fast bacilli. The cases were diagnosed as tuberculosis and treated with orchiectomy and antituberculous drugs. The patients did well. Histologic sections confirmed the cytologic diagnosis. CONCLUSION: FNAC is useful in the diagnosis of tuberculous orchitis.